HLAG (HLA-G) (C-term) Mouse Monoclonal Antibody [Clone ID: 5A6G7]

Specifications

Product Data
Clone Name	5A6G7
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	Western blot (Reducing Conditions): 12% AA SDS-PAGE. Positive Control: JEG-3 cell lysate. Flow Cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining. Immunohistochemistry on Paraffin and Frozen Sections: 10-20 μg/ml. Positive tissue: placenta tissue. Immunocytochemistry. ELISA: The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody AM03005BT-N. Positive Control: HeLa/HLA-G5 transfectants cell lysate. HeLa/HLA-G5 cell supernatant. Negative Control: HeLa cell lysate.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin
Specificity	The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Isotype	IgG1
Formulation	PBS, pH 7.4 with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Protein A chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	major histocompatibility complex, class I, G
Database Link	Entrez Gene 3135 Human UniProt ID P17693
Background	HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
Synonyms	HLA-6.0, HLAG, MHC class I antigen G
Reference Data

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