gamma Tubulin (TUBG1) Mouse Monoclonal Antibody [Clone ID: TU-30]

Specifications

Product Data
Clone Name	TU-30
Applications	IF, WB
Recommend Dilution	Western blot. Immunocytochemistry (1-2 μg/ml) Staining Technique: a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Incubation Time: 45 min in room temperature. Positive Control: P-19 Mouse embryonal carcinoma cell line 3T3 mouse fibroblasts Note: The antibody TU-30 stains only fixed cells.
Reactivity	Bovine, Chicken, Human, Mouse, Plant, Porcine, Rat, Protozoa
Host	Mouse
Clonality	Monoclonal
Immunogen	C-terminal peptide (aa 434-449) of gamma-Tubulin counjugated to KLH
Specificity	The antibody TU-30 recognizes C-terminal peptide sequence of gamma-Tubulin.
Formulation	Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative State: Aff - Purified State: Liquid purified Ig fraction (> 95% (by SDS-PAGE)
Concentration	lot specific
Purification	Protein-A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	tubulin gamma 1
Database Link	Entrez Gene 7283 Human UniProt ID P23258
Background	The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
Synonyms	Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1
Reference Data

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