HLAE (HLA-E) Mouse Monoclonal Antibody [Clone ID: MEM-E/08]

Specifications

Product Data
Clone Name	MEM-E/08
Applications	FC, IP
Recommend Dilution	Flow Cytometry: 4 µg/ml. Positive control: human cell line LCL 721.221 (HLA-E transfected). Immunoprecipitation.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide
Specificity	The antibody MEM-E/08 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. The published results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A-affinity chromatography (purity > 95% by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	major histocompatibility complex, class I, E
Database Link	Entrez Gene 3133 Human UniProt ID P13747
Background	HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
Synonyms	HLA-6.2, HLAE, MHC class I antigen E
Reference Data

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