Keratan-Sulfate Mouse Monoclonal Antibody [Clone ID: 4B3/D10]

Specifications

Product Data
Clone Name	4B3/D10
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA (less than 1 µg/ml). Western blot (1 µg/ml). Immunohistochemistry (1 µg/ml).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human Aggrecan
Specificity	This Monoclonal 4B3/D10 antibody is specific for Keratan Sulfate Glycosaminoglycan chains. Preliminary results, based on western blot analysis of keratan sulfate from different sources, indicate that mAb 4B3/10 is specific for keratan sulfate from articular cartilage and shows only minimal if any cross-reac-tivity with keratan sulfate from intervertebral disc. This is in contrast to the epitope recognized by the known mAb 5D4, that recognizes a widely distributed keratan sulfate epitope. Therefore, in addition to appliations in Immunochemistry and Immunohistochemistry, the mAb 4B3/D10 is well suited for detecting keratan sulfate fragments released during human inflammatory or degenerative joint diseases into synovial fluid and serum.
Isotype	IgG1
Formulation	50 mM TRIS pH 7,4 State: Purified State: Lyophilized purified IgG fraction from Cell Culture Supernatant
Reconstitution Method	Restore in aqua bidest to 1 mg/ml.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	Aggrecan is the major proteoglycan of human articular car-tilage. The core protein is substituted by a number of keratan sulfate and chondroitin sulfate glycosaminoglycan chains. Whereas chondroitin sulfate is widely distributed throughout the body, keratan sulfate is primarily expressed in cartilage (joints, trachea, intervertebral discs) and cornea.
Reference Data

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