CEACAM6 Mouse Monoclonal Antibody [Clone ID: 9A6]

Specifications

Product Data
Clone Name	9A6
Applications	ELISA, FC, IHC, WB
Recommend Dilution	ELISA:1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400. Western blot: 4 µg/ml. Flow Cytometry: 1.2 µg/106 cells. Immunohistology on Cryo-Sections: 1-2 µg/106 cells. For various aplications see list of References.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Tumor cell lines expressing CEACAM6 (1,2)
Specificity	9A6 recognizes exclusively CEACAM6 transiently expressed on the cell surface of transfected BOSC cells (Fig. 1). 9A6 can be used to distinguish CEACAM6 from all other CEACAM and probably all pregnancy-specific glycoproteins molecules, namely CEACAM1 (BGP/CD66a), CEACAM3 (CGM1/CD66d), CEACAM4 (CGM7), CEACAM6 (NCA/CD66c), CEACAM7 (CGM2), CEACAM8 (CGM6/CD66b) and PSG1 (CD66f) based on its reactivity pattern with stable HeLa transfectants expressing individual CEA family members (3 and Fig.1). 9A6 was included and characterized in the framework of the VIth Leucocyte Typing Workshop.
Formulation	PBS, pH 7.2 without preservatives State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	carcinoembryonic antigen related cell adhesion molecule 6
Database Link	Entrez Gene 4680 Human UniProt ID P40199
Background	CEA-related cell adhesion molecule 6 (CEACAM6, NCA) belongs to the carcinoembryonic antigen (CEA) gene family (13). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 90,000 which is strongly expressed on epithelial cells of the fetal and adult gastrointestinal tract, epithelia of glandular tissues, squamous epithelial cell of the tongue, esophagus and cervix as well as on granulocytes (Fig. 2; Ref. 4, 10). CEACAM6 expression is upregulated in many adenocarcinomas (4) and leukemias (5). Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by one immunoglobulin constant-like A and B domain.
Synonyms	Normal cross-reacting antigen, Non-specific crossreacting antigen, NCA
Reference Data

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