Complement C4A (C4A) Mouse Monoclonal Antibody [Clone ID: 10-12]

Specifications

Product Data
Clone Name	10-12
Applications	ELISA, FC, IHC, WB
Recommend Dilution	Western Blot. Flow Cytometry. ELISA: This antibody binds both C4c and C4 and so is not suitable for all ELISA applications. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Recommended Postive Control: Kidney sections from post-streptococal glomerulonephritis pastients.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human Complement C4c.
Specificity	This antibody recognises complement component 4c (C4c).
Isotype	IgG1
Formulation	Borate buffered saline, pH 8.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement component 4A (Rodgers blood group)
Database Link	Entrez Gene 720 Human UniProt ID P0C0L4
Background	The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4bs alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kDa) and alpha 3 (25 kDa) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma.
Synonyms	Complement Component 4, CPAMD2, CPAMD3
Reference Data
Protein Pathways	Complement and coagulation cascades, Systemic lupus erythematosus

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