CD79A (202-216) Mouse Monoclonal Antibody [Clone ID: HM57]

Specifications

Product Data
Clone Name	HM57
Applications	FC, IHC, WB
Recommend Dilution	Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human tonsil. Flow Cytometry: Use 10 µl of 1/10-1/25 diluted antibody to label 1x106 cells in 100 µl (Membrane permeabilization is required).
Reactivity	Bovine, Canine, Chicken, Equine, Guinea Pig, Human, Monkey, Mouse, Porcine, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to 202-216 amino acid sequence of Human mb-1.
Specificity	This antibody recognizes an epitope within the cytoplasmic domain of CD79a.
Isotype	IgG1
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD79a molecule
Database Link	Entrez Gene 973 Human UniProt ID P11912
Background	CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin.
Synonyms	IGA, MB1, B-Cell marker
Reference Data

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