Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: Ki-67]

Specifications

Product Data
Clone Name	Ki-67
Applications	FC
Recommend Dilution	Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity	Bovine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Nuclei of the Hodgkin lymphoma cell line L428
Specificity	This antibody recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS) Label: PE State: Liquid purified Ig fraction Preservative: 15 mM sodium azide Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	marker of proliferation Ki-67
Database Link	Entrez Gene 4288 Human UniProt ID P46013
Background	Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Synonyms	Ki67 antigen, MKI67, Proliferation marker
Reference Data

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