DHLAG (CD74) Mouse Monoclonal Antibody [Clone ID: Bu45]

Specifications

Product Data
Clone Name	Bu45
Applications	FC, FN, IF, IHC, IP
Recommend Dilution	Immunoprecipitation. Immunohistochemistry on Frozen Sections: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. Flow Cytometry: Use 10µl of 1/25-1/50 diluted antibody to label 1x106 cells in 100 µl.  Membrane permeabilisation may enhance CD74 staining.  Functional Assays: Removal of Sodium Azide by dialysis is recommended prior to use. Dialysis cassettes are suitable for this purpose.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	B lymphoblastoid cell line HFB1. Spleen cells from immunised mice were fused with cells of the Ag8.653 myeloma cell line.
Specificity	This antibody recognizes Human CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kDa isoforms of CD74.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD74 molecule
Database Link	Entrez Gene 972 Human UniProt ID P04233
Background	The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus.
Synonyms	p33, DHLAG
Reference Data

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