LOC396098 Mouse Monoclonal Antibody [Clone ID: L22]

Specifications

Product Data
Clone Name	L22
Applications	FC
Recommend Dilution	Flow Cytometry: 1/20 - 1/80; Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Immunogen	Chicken Bursa cells
Specificity	MCA2170F recognises the 'a' allele of the polymorphic cell surface antigen Bu-1.
Isotype	IgG1
Formulation	BPS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified Ig
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Name	B6.1
Database Link	Entrez Gene 396098 Chicken UniProt ID Q90746
Background	Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development. Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.
Synonyms	B-Cell Marker
Reference Data

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