Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: PY20]

Specifications

Product Data
Clone Name	PY20
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Suitable for Immunohistochemistry (5-10 ug/ml) and Immunocytochemistry (5-10 ug/ml).
Reactivity	Broad
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
Specificity	This antibody specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
Isotype	IgG2b
Formulation	0.02 M Sodium Phosphate, pH 7.5 with 0.15 M Sodium Chloride, 3 mM Sodium Azide as preservative, 50% glycerol Label: TAMRA State: Liquid purified IgG fraction. Label: The antibody was labeled by reaction with tetramethyl-rhodamine succinimidyl ester in the presence of phenyl phosphate (to protect the antibody binding site). The unreacted succinimidyl ester was removed by molecular exclusion chromatography
Concentration	lot specific
Purification	Affinity purification on a column of immobilized phosphotyrosine.
Conjugation	TAMRA
Storage Condition	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation. Its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The rhodamine conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
Reference Data

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