MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10B1]

Specifications

Product Data
Clone Name	10B1
Applications	ELISA, WB
Recommend Dilution	Western Blot: 1μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive Control: Cell lysate from untreated SW480 cells. ELISA: 0.05 μg/ml.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH
Specificity	This antibody specifically recognizes the N-terminus of MEK1 at 45 kDa.
Isotype	IgG1
Formulation	PBS/0.09% Na-Azide/PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (aliquote in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	mitogen-activated protein kinase kinase 1
Database Link	Entrez Gene 5604 Human UniProt ID Q02750
Background	MEK (MAP Kinase Kinase) phosphorylates the MAP kinase on both threonine and tyrosine residues of the activation loop motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.
Synonyms	MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1
Reference Data

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