N-Acetylglucosamine (GlcNAc) Mouse Monoclonal Antibody [Clone ID: 10D8]

Specifications

Product Data
Clone Name	10D8
Applications	ELISA, WB
Recommend Dilution	Western blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 - 1.0 µg/ml.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	N-Acetylglucosamine conjugated to KLH.
Specificity	This antibody reacts to N-Acetylglucosamine.
Isotype	IgM
Formulation	1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgM
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt store lyophilized (preferably in a desiccator) at -20°C for long-term. After reconstitution aliquote and freeze (in liquid nitrogen) at -80°C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Background	Many intracellular proteins contain monomeric O - linked N - acetylglucosamine residues conjugated to th hydroxyl group of serine or threonine residues. This modification can interfere with tserine and threonine phosphorylation.
Reference Data

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