ERK5 (MAPK7) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12F2]

Specifications

Product Data
Clone Name	12F2
Applications	WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to Hemocyanin. Epitope: N-Terminus.
Specificity	This antibody recognizes MAP kinase 7 (erk5) at 92 kDa in Western-blot applications.
Formulation	1 ml PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	mitogen-activated protein kinase 7
Database Link	Entrez Gene 5598 Human UniProt ID Q13164
Background	Erk5 (also known as MAPK7 or big mitogen activated kinase 1, BMK1) belongs to the Ser/Thr protein kinase family. It contains a T E Y motif in the activation loop similar to Erk1/2. Extracellular signals, including receptor tyrosine kinases and G-protein-coupled receptors as well as osmotic and oxdative stress lead to Erk5 activation by MEK5. The Erk5 pathway plays an important role in cellular proliferation, differentiation and survival.
Synonyms	Mitogen-activated protein kinase 7, Extracellular signal-regulated kinase 5, ERK4, Big MAP kinase 1, MAPK7
Note	Protocol: Positive Control Cell Lysate: SKOV-3 Untreated Format: Lyophilized cell lysate from Serum starved SKOV-3 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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