beta Catenin (CTNNB1) (core) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9G10]

CAT#: AM00021PU-N

beta Catenin (CTNNB1) (core) (incl. pos. control) mouse monoclonal antibody, clone 9G10, Purified



Need it in bulk or conjugated?
Get a free quote

CNY 5,148.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 9G10
Applications ELISA, IF, IHC, IP, WB
Recommend Dilution ELISA: 0.05 μg/ml.
Immunoprecipitation: 1-10 μg per 106 vanadate treated A431 cells.
Immunocytochemistry: 0.1-1 μg/ml. 
Immunohistochemistry on Paraffin Sections.
Western Blot: 0.5 μg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation
buffer.
Included Positive Control: Cell Lysate from Untreated SW480 cells:
Formulation: Lyophilized Cell Lysate from Serum starved SW480 Cells.


Stability: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDSPAGE
sample buffer, mix and incubate at 90°C for 5 min.


Application: The Positive Control cell lysate is recommended for immunoblot applications. 20 μl of
positive control cell lysate corresponds to ca 20.000 cells.
Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins.


Note: The lyophilized cell lysates contain SDS and are not recommended for applications
with native proteins such as Immunoprecipitation.


Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.
Shelf life: one year from despatch.

Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Recombinant β-Catenin. 
Epitope: Core (armadillo repeats).
Specificity This antibody specifically interacts with the core region (armadillo repeats) of β-Catenin.
Formulation 1 ml 2 x PBS
State: Purified
State: Lyophilized purified Ig fraction from Serum-Free Cell Culture Supernatant
Stabilizer: PEG and Sucrose
Preservative: 0.09% Sodium Azide
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subesequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size 90 kDa
Gene Name catenin beta 1
Background The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity.
Synonyms CTNNB1, CTNNB, Beta-catenin
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...