Cadherin-15 (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 12G4]

Specifications

Product Data
Clone Name	12G4
Applications	ELISA, IF, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml. Western Blot (Immunoblotting): 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Immunocytochemistry: 5 µg/ml.
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant extracellular domain of Murine M-Cadherin
Specificity	This antibody is directed against the extracellular domain of Murine M-Cadherin (MW: 130 kDa).
Isotype	IgG1
Formulation	1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	130 kDa
Gene Name	cadherin 15
Database Link	Entrez Gene 12555 Mouse UniProt ID P33146
Background	Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin. M-cadherin is expressed in myoblasts and might be involved in skeletal muscle cell differentiation.
Synonyms	Muscle cadherin, Cadherin-14, CDH15, CDH14, CDH3, Cadherin M, M-Cadherin
Reference Data

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