Amyloid beta (free N-term, APP reactive) Mouse Monoclonal Antibody [Clone ID: 19H5]

Specifications

Product Data
Clone Name	19H5
Applications	ELISA, WB
Recommend Dilution	ELISA: 0.05 µg/ml. Western blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	N-terminal peptide conjugated to KLH.
Specificity	This antibody recognizes the free N-Terminus of the βA4 polypeptide. The antibody crossreacts with APP.
Isotype	IgG1
Formulation	1 ml 2 x PBS containing PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at -20°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -80°C for longer. Avoid repeated freezing and thawing.
Background	The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimers disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).
Reference Data

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