TRIML2 (NM_001303419) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SPRYD6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336266 representing NM_001303419. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCAAAAGGCTCAGCCCTCAGTTACAGCACAACATCACAGAAGATGCCTATTGTGAAACACACCTG GAACCAACACGGCTGTTCTGTGATGTTGACCAAATCACACTCTGCAGCAAATGCTTCCAGTCCCAGGAG CACAAACATCACATGGTGTGTGGGATACAAGAGGCTGCTGAGAATTACAGGAAGTTATTCCAGGAAATA TTGAACACATCGAGGGAGAAACTTGAAGCAGCTAAAAGCATATTGACTGATGAGCAAGAAAGAATGGCG ATGATTCAGGAAGAGGAACAAAATTTTAAAAAGATGATTGAGTCTGAGTATAGTATGAGACTCCGGTTG TTGAATGAAGAGTGTGAGCAGAATCTCCAGAGACAGCAGGAATGCATATCTGACTTGAACCTGAGAGAA ACCCTTCTGAATCAAGCGATCAAGCTTGCCACCGAGCTAGAGGAGATGTTCCAGGAAATGCTACAGAGA CTGGGCCGTGTGGGGAGAGAGAACATGGAGAAACTGAAGGAGAGTGAAGCCAGGGCTTCTGAACAGGTC CGCAGCCTCCTAAAGCTCATCGTGGAGCTTGAGAAAAAGTGTGGGGAAGGCACCTTGGCATTGCTCAAG TGCCTTGATCTTGAAATTCGGCCTCCAGAACTGCGAGAAATGAATTTCTGCTGTGCAGAAGCTACTCAG TCTATGAATGCAAAATACTCTTTAGAAAGGAGCAAGTCACTGCTGCTTGAGCATCTGGAGCCCGCTCAT ATCACAGACCTGAGTTTATGCCACATAAGAGGACTCAGCAGCATGTTCAGAGTACTCCAGAGACATTTA ACATTGGATCCTGAAACAGCTCATCCCTGCCTGGCACTATCTGAGGACCTGAGAACTATGAGATTGAGA CATGGGCAGCAGGATGGGGCTGGCAACCCAGAAAGATTGGATTTCAGTGCCATGGTGCTGGCTGCGGAG AGCTTCACCTCAGGGAGGCACTACTGGGAGGTGGACGTGGAAAAGGCAACCAGGTGGCAAGTGGGCATA TACCACGGCTCTGCAGACGCGAAGGGCAGCACGGCCAGAGCTTCCGGAGAGAAAGTCTTGCTCACGGGG TCGGTGATGGGGACCGAGTGGACTCTCTGGGTCTTCCCCCCTCTGAAAAGGCTCTTCCTGGAAAAGAAG TTGGACACAGTTGGCGTTTTCCTTGACTGCGAACACGGGCAGATATCATTCTACAATGTGACCGAGATG TCCCTCATTTACAATTTCTCCCATTGCGCCTTCCAAGGAGCTCTCAGGCCTGTGTTTTCCCTCTGTATC CCAAATGGAGACACAAGTCCAGACTCCCTCACCATCTTACAACATGGTCCTTCTTGTGATGCTACTGTT AGCCCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303419
Insert Size	1389 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303419.1
RefSeq Size	1716 bp
RefSeq ORF	1389 bp
Locus ID	205860
UniProt ID	Q8N7C3
Protein Families	Druggable Genome
MW	52.8 kDa
Gene Summary	This gene encodes a member of the tri-partite motif (TRIM) family of proteins. This protein may be regulated by the tumor suppressor p53 and may regulate p53 through the enhancement of p53 SUMOylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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