PCGF5 (AK092045) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C3orf6; DFNA44; YMER
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092045, the custom clone sequence may differ by one or more nucleotides GTTTTGTTTTGTTTTTGGAGTTGGAGTCTCGCCCTGTCACACAGGCTGGAGTGCAGTGGTGCAATCTTGG CTCACTGCAACCTCCGCCACCTGGGCTGGAGCAATTCCCCTGCCTCAGCCTCCCAAGTAGCTGGAATTAC AGGCATGTGTCACCACACCCGGCTAATTTTTGTATTTTTAGTAAAGAGCGGGTTTCACCATGTTGGCCAG GCTGGTCTCGAACCCCTGACCTCAAGTGATCCACCTGCCTTGGCCTCCCAAGGTGCTGGGATTACAGGCT TAAGCCACCATGTCCAGCCAGCCAAGTATTTGGTTTTAAACATTTGTATTCCTTTGTGCAAGGGATTGTA TTCCTCTGTACAAGACCTTTAAGCACCTCTTCAAGAAATTTAGTCTTTGTGTAGCTGCTAACCTTACTGC AATATTTATTTTCCCTTATTTTCCCATACTACTTAGTTGACAAATGGCAATCAAGCTCACTTGTTCTCCC TCTCTCTGTCTCCCCCCTATGTACACATATATATATCCCTTCAGCTTGACTTGCTATTATTTTTCATTGT ATAAATATGAAATTCTTGGCATTTCAATATGGCATTTGTTTAGGGAAAAAATATGTAGGTCACTAGAGAA AAATGTTAATTTTTTTCCCACTGTGAATTGCCTTCTGTTTGCCTTATCTTTTCAGATAGTCTTTAAAGTT GGGCAATATTCAGGCAGCTATTTAGAACAGTTTCTCAGAGTGGATTTGGGTCTCTCATTTGAGAAAGATT TGTAGTTTCAATGAAGTTTGTATATTATAGGAATTTAAGTAAAATCATCTGAGTTAAGTTTTGGGCAAAT GTTAATCAGAAAGATAATCTACATCCCTACTTTATGTAGTCATTTTGGAGAAATGGAGGAAAAGGAAAAT AACAGGACTTTTTATAGCTGTGATAAAATGGGTAAAGAAAAACCTCCATTTAAATAAGGAAGGGGAGACA AAATGGAAGGTCCTGGCCTTAAGTCAAATTCCAGATAGTGCTTTCAATATCCAAAGCTTAGGCCTTGTTT ACTTATAGTAATGCTATATTTTTCTTTATCTGCCCTGTGATTTCGCTTCGTGTCCTTCAAAGTTTTTTTA TTGTAATTTTAAAGGAAAAAAGCAATGTATTTTTATTTGATAACTAGGCAAAATCATTTGCCATTTTTTA TTTCATAAATATTTATTTTTCTATCTCATACAATGATCAGTTTTACTTTAAAAATTCTGCATTTCTCTTG CCTCTCATGTACTTCATCCAGTTATTTATTATTGTAAAATGTTAACTGGAGATACTGCATTCAAAAAAAA GTCGGACCACTTAACACTTACCAGGAATGTTAGTGTTTCAGCTGTTTTAGTAAGTGACCAAGTAAGCTAT CACCTTGTGATAGCTAATTCAACATCTTTATTGAAAATTTGTTACTTTTTTTAAAGGTTTTTAAAAGATT ACAATTTTAAAATATTTTATGTTTATTTTATTTTTGTGTAAAGCAAACACTTTTTAAATAATATCAGAGT AATTATTACCCTATGAATAAGGAGATAGAAATGAAATTCAGCCAGTTAAATCCTCATACTCTTTCTCATT CAAATAATATAATAATGTTTGAGACTATAGAAGCAACTCAACTGTATTTTAATGAACTGCCAGACACTTT TCACTGTGTTCTCTGCTTTTTACTTTGTCATTTTTATATAAATGTGAAAGCATTTTGTTGTTGTTGTTGT TGTTCCTGCTGCTAGAACACATGCAGTTTAATTTTTAGGCCCACAGTGTATTATGCTTTAATGTTGTGAT GTAAACTAATACTTCTGGCCTGGGAAAACCTTTCTGTTTCTGTATCTTTGTCCTTTGGAGGCCAGGCCTC ATTAACACCAGTATTGTTGAATAAGCACAATATATGACGGAGCCTTATCATAAGGCTGCTTGACATAGAC ACCCACTCTCCTCCACATTTTATAAATCATTGTTTGGAAGTAAAGTTAGATAAGAGTCAAATTAAATGTT TTGACTTGAAAAATATTTGACTTTTGCGTTCAAACCACTGTTAGACGTAAGTATTTTATTAGACATAAGT ATTTTATTTTGGGACTAGACAGTTGCTTTACATGTATTTAAGGGAACATTGTGATTTTCAGAAAGTGGCT TTACATACTATTGAATTGCTATTTCCCAAGTTACTTTACAACCACCAGCAGCATACCAATAAATTACAAA AGCAGAAAATGAAAAAAAAAAAAAAAAAAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092045 unedited GGGTTCACAATTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGTGTGTCAC CACACCCGGCTAATTTTTGTATTTTTAGTAAAGAGCGGGTTTCACCATGTTGGCCAGGCT GGTCTCGAACCCCTGACCTCAAGTGATCCACCTGCCTTGGCCTCCCAAGTGCTGGGATTA CAGGCTTAAGCCACCATGTCCAGCCAGCCAAGTATTTGGTTTTAAACATTTGTATTCCTT TGTGCAAGGGATTGTATTCCTCTGTACAAGACCTTTAAGCACCTCTTCAAGAAATTTAGT CTTTGTGTAGCTGCTAACCTTACTGCAATATTTATTTTCCCTTATTTTCCCATACTACTT AGTTGACAAATGGCAATCAAGCTCACTTGTTCTCCCTCTCTCTGTCTCCCCCCCATGTAC ACATATATATATCCCTTCAGCTTGACTTGCTATTATTTTTCATTGTATAAATATGAAATT CTTGGCATTTCAATATGGCATTTGTTTAGGGAAAAAATATGTAGGTCACTAGAGAAAAAT GTTAATTTTTTTCCCACTGTGAATTGCCTTCTGTTTGCCTTATCTTTTCAGATAGTCTTT AAAGTTGGGCAATATTCAGGCAGCTATTTAGAACAGTTTCTCAGAGTGGATTTGGGTCTC TCATTTGAGAAAGATTTGTAGTTTCAATGAAGTTTGTATATTATAGGAATTTAAGTAAAA TCATCTGAGTTAAGTTTTGGGCAAAATGTAATCAGAAAGATAATCTACATCCCTACTTTA TGTAGTCATTNTGGAGAAATGGANGAAAAGGNAAAATACANGACTTTTTATAGCTGTGAT AAAATGGGTAAAGAAAAACCTNCATTTAAAATAGAAGGGGAGACAAATGGAAGGTNCTGN NNCTAAGTCAATTCCAGATAGTGC
Restriction Sites	NotI-NotI
ACCN	AK092045
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK092045.1
RefSeq Size	2271 bp
RefSeq ORF	2271 bp
Locus ID	84333
Gene Summary	Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:26151332). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332). Plays a redundant role with PCGF3 as part of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (By similarity).[UniProtKB/Swiss-Prot Function]

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