Cacna1b (NM_001195199) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BIII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217766 representing NM_001195199 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGCG GCGGGGCCGGCGGGGCCGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA GTCCATTGCGCAACGCGCACGGACCATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATATGCTAAGCGCATCACCGAAT GGCCGCCCTTCGAATATATGATCCTGGCCACCATCATCGCCAACTGTATTGTCCTGGCCCTGGAGCAGCA CCTCCCTGATGGGGACAAGACTCCCATGTCTGAACGACTGGATGACACGGAACCTTACTTCATCGGCATC TTTTGCTTCGAGGCGGGCATCAAGATCATAGCTCTGGGCTTCGTGTTCCACAAAGGCTCCTACCTCCGGA ATGGCTGGAACGTCATGGACTTCGTGGTGGTCCTCACAGGGATTCTTGCCACAGCTGGAACTGACTTTGA TCTGCGCACCCTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGTTGGTGTCTGGAATTCCAAGCTTGCAG GTGGTGCTCAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATCGGGCTGCTGCTCTTCTTCGCCA TCCTCATGTTCGCTATCATCGGCCTCGAGTTCTATATGGGCAAATTCCATAAGGCCTGCTTCCCCAACAG CACAGATGCAGAGCCTGTGGGTGACTTTCCTTGTGGCAAGGAGGCCCCTGCTCGTCTGTGTGACAGTGAC ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA TCTTGACCGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC CGGCAACACGTGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG GTGCTCGGTGTGCTTTCAGGAGAGTTTGCCAAAGAGCGGGAGCGAGTCGAGAACCGCCGTGCCTTCCTGA AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAACTGAATGGGTACTTGGAGTGGATCTTCAAGGCGGAGGA AGTCATGTTGGCAGAGGAGGACAAGAACGCAGAAGAGAAGTCCCCTTTGGATGCAGTGTTGAAGAGAGCT GCTACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTG CTGCTGGGTCTCCCTTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACAGAGAGCTCATCGTACTTCCGGAG GAAGGAGAAGATGTTCCGGTTCCTTATCCGTCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTG TGCGTGGTGGCCCTGAACACGTTGTGTGTGGCCATGGTACACTATAATCAGCCTCAGCGGCTTACCACTG CACTGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTACGGTCT AGGGCCCAGAAGCTACTTCCGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTT GAAGTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTCGGAATCAGTGTGCTGCGGGCTCTCCGACTGC TGAGGATTTTCAAAGTCACCAAGTATTGGAACTCCCTGAGGAACCTGGTTGTTTCCCTCCTCAACTCCAT GAAGTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTTTTCATTGTGGTCTTCGCTCTGTTGGGGATGCAG CTGTTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCA TCCTCACTGTGTTTCAGATTCTGACAGGAGAGGACTGGAATGCAGTCATGTATCATGGGATTGAGTCACA AGGAGGAGTCAGCAAAGGCATGTTTTCATCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACC CTGTTGAACGTTTTCTTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAG AGGAGATGGAAGAGGCAGCCAATCAGAAGCTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCC CATGTCTGCTGCCAACATCTCCATTGCTGCTTTTGTAAAGCAAACTCGAGGTACTGTATCTCGCAGCTCA TCTGTCTCCAGCGTAAACTCACCGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAGCGGGCCA GTCAGCTAAGGCTCCAGAACCTGCGTGCCAGCTGTGAGGCACTGTACAGTGAGATGGACCCGGAGGAGCG CCTGCGTTATGCCAGCACGCGCCACGTGAGGCCAGACATGAAGACACACATGGACCGACCCCTAGTGGTG GAACCTGGTCGGGATGGCCTGCGGGGACCCGCCGGGAACAAGTCAAAGCCTGAGGGCACGGAGGCCACCG AAGGTGCGGATCCACCACGCCGACACCACCGGCATCGTGATAGGGACAAGACCTCAGCCTCAACCCCTGC TGGAGGCGAACAGGACAGGACAGACTGCCCAAAGGCCGAAAGCACCGAGACCGGGGCCCGGGAGGAACGT GCGCGCCCTCGTCGAAGTCACAGCAAGGAGGCTCCAGGGGCTGACACACAAGTGCGTTGTGAGCGCAGTA GACGTCACCACCGGCGCGGATCCCCGGAGGAGGCCACTGAACGGGAACCTCGGCGCCACCGTGCCCACCG GCACGCACAGGACTCAAGCAAGGAAGGCAAGGAGGGCACTGCACCGGTGCTTGTACCCAAGGGCGAGCGT CGCGCAAGACATCGAGGCCCGCGTACGGGCCCCCGTGAGACAGAGAACAGTGAGGAGCCCACACGCAGGC ACCGTGCAAAGCATAAGGTGCCACCAACACTTGAGCCCCCAGAGAGGGAGGTTGCAGAGAAGGAGAGCAA CGTGGTGGAAGGGGATAAGGAAACTCGAAATCACCAGCCCAAGGAACCTCGCTGTGACCTGGAGGCCATT GCGGTTACAGGCGTGGGCTCTCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAACAGCCAG AGGATGCAGACAACCAGCGTAATGTCACCCGGATGGGCAGTCAGCCCTCAGACCCCAGCACCACTGTGCA TGTCCCAGTGACACTGACAGGCCCTCCCGGGGAGGCCACTGTAGTTCCCAGTGCTAACACGGACCTGGAA GGCCAAGCGGAGGGCAAGAAGGAGGCAGAGGCTGACGATGTGCTGAGAAGAGGCCCCAGGCCCATCGTTC CCTACAGTTCCATGTTCTGCCTCAGCCCCACCAACCTACTCCGTCGCTTCTGCCATTACATTGTGACCAT GCGGTACTTTGAGATGGTGATTCTTGTGGTCATCGCCTTGAGCAGCATTGCCCTGGCTGCTGAGGATCCC GTGCGGACCGACTCATTCCGGAACAATGCTCTGAAGTACATGGACTACATCTTTACAGGAGTCTTCACCT TTGAGATGGTCATAAAGATGATAGACTTGGGCCTGCTGCTGCACCCTGGGGCCTACTTCCGGGACCTGTG GAACATTCTGGACTTCATTGTTGTCAGTGGAGCCCTGGTGGCATTTGCATTCTCAGGATCCAAAGGGAAA GACATCAATACCATCAAGTCTCTGAGAGTCCTGCGAGTCCTGCGGCCCCTCAAGACCATCAAGCGGCTGC CTAAACTCAAGGCTGTGTTTGACTGTGTGGTGAACTCTCTGAAGAATGTCTTGAACATCCTGATCGTCTA CATGCTCTTCATGTTTATATTTGCCGTCATCGCCGTCCAACTCTTCAAAGGGAAGTTCTTTTACTGCACT GATGAGTCCAAGGAGCTGGAGCGGGACTGCAGGGGTCAGTATTTGGATTATGAGAAGGAAGAGGTAGAAG CCCAGCCAAGGCAGTGGAAGAAATATGACTTCCACTATGACAATGTGCTCTGGGCCTTGCTGACTCTGTT TACGGTGTCCACAGGAGAGGGGTGGCCCATGGTGCTGAAACACTCTGTGGACGCCACCTATGAGGAGCAG GGGCCAAGCCCCGGGTTTCGGATGGAGCTTTCCATCTTCTATGTGGTCTACTTTGTGGTCTTCCCTTTTT TCTTTGTCAACATCTTTGTGGCCTTGATCATCATCACCTTCCAGGAGCAGGGGGACAAGGTGATGTCTGA GTGCAGTCTGGAAAAGAATGAGAGGGCTTGCATTGACTTTGCCATCAGCGCCAAACCCCTGACACGGTAC ATGCCTCAGAACAAGCAGTCGTTCCAGTATAAGACATGGACATTTGTGGTCTCTCCACCCTTTGAGTACT TCATTATGGCCATGATAGCCCTCAACACAGTGGTGCTGATGATGAAGTTCTACGATGCCCCTTATGAGTA CGAGCTGATGCTGAAGTGCTTGAACATCGTCTTCACATCCATGTTCTCTCTGGAGTGCATCCTGAAGATC ATCGCCTTCGGGGTGTTGAACTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACTGTTTTGGGAA GTATTACTGATATTTTAGTAACGGAGATTGCGGAAACGAACAACTTCATCAACTTGAGCTTCCTTCGCCT CTTCCGGGCAGCACGGCTGATCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCTTGTTATGGACCTTT GTCCAGTCCTTTAAGGCGCTGCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTACGCCATCA TCGGCATGCAGGTTTTTGGAAACATTGCCCTTGATGATGGCACCAGCATCAACCGACACAACAACTTCCG GACATTTCTGCAAGCCTTAATGCTGTTGTTCAGGAGTGCCACTGGGGAGGCCTGGCACGAAATCATGCTG TCTTGCCTGGGCAACCGGGCCTGCGACCCACATGCCAACGCCAGCGAATGCGGGAGCGACTTTGCCTATT TTTATTTTGTCTCCTTCATCTTCCTCTGTTCCTTTCTGATGCTGAACCTCTTTGTTGCTGTGATCATGGA CAATTTCGAATACCTCACGCGGGATTCTTCCATCCTAGGGCCGCACCACCTCGATGAATTCATTCGCGTC TGGGCTGAATACGACCCAGCTGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTGAAACACA TGTCCCCACCTCTGGGTTTGGGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTCGAATGAA CATGCCCATATCCAATGAGGACATGACGGTACACTTTACATCCACACTGATGGCCCTCATCCGGACGGCA CTGGAGATCAAGCTTGCCCCAGCGGGGACAAAACAGCACCAATGTGATGCTGAGCTGAGGAAGGAGATCT CTTCTGTGTGGGCTAATCTGCCCCAGAAGACTCTGGACTTACTGGTGCCACCCCACAAACCTGACGAGAT GACAGTGGGGAAGGTCTATGCGGCTCTCATGATATTTGACTTCTACAAACAGAACAAAACCACCAGAGAT CAGACTCACCAAGCTCCTGGAGGCCTGTCCCAGATGGGTCCTGTTTCCCTGTTCCATCCTCTGAAGGCCA CCCTGGAGCAGACACAGCCCGCTGTGCTCCGAGGAGCTCGGGTTTTCCTTCGACAAAAGAGTGCAACTTC CCTCAGCAATGGGGGCGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCCCTGTCCTGGGGCACGCAG AGGACCCAGGACGTACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAGAGATCCCTGTGG GGCAGCCAGGAGCACTGGCTGTAGATGTCCAGATGCAGAACATGACATTGAGAGGACCGGATGGGGAGCC CCAGCCTGGCCTGGAGAGCCAAGGCCGAGCGGCCTCTATGCCACGCCTGGCGGCAGAAACACAGCCGGCC CCTAATGCCAGCCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCGCATGGGACTCAGCTTTGCA ACACAGTCCTGGACCGGCCACCTCCTAGCCAGGTGTCCCATCACCACCACCACCGCTGCCACCGGCGCAG GGACAAGAAGCAGAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACACAGAAGGTGCACCAAGTACT GCTGCAGGATCTGGCCTGCCCCATGGAGAAGGGTCCACAGGCTGCCGGCGGGAGCGTAAGCAAGAGCGAG GCCGGTCCCAGGAGCGGAGGCAGCCCTCCTCCTCTTCTTCAGAGAAGCAGCGCTTCTATTCCTGTGACCG CTTTGGGAGCCGGGAGCCCCCACAACCTAAGCCCTCCCTCAGTAGCCACCCCATATCGCCAACAGCGGCA CTAGAGCCAGGACCCCACCCGCAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGTCAACATCTGGTG CTAGCACGCCGGGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCCCTGACCCCACGCCCCAGCATCAC CTACAAGACGGCCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGTGGCCTTCCAGCCTTCTCCCCT GGCCGTCTCAGCCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGAGCCAGCCTCTAG CTTCTGGCTCCCGCATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGCCTCTGCCCACAA CCTGCCTGAGGATACACTCACCTTTGAAGAGGCCGTGGCCACCAACTCTGGCCGCTCCTCCAGGACTTCC TATGTGTCCTCCCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTACCACTGCACTTTGG GACTCAGCACCGGCGTCCGGGCGCGGCACAGCTACCACCACCCAGACCAGGATCACTGGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217766 representing NM_001195199 Red=Cloning site Green=Tags(s) MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFT VNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGI FCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQ VVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCDSD TECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNL VLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRA ATKKSRNDLIHAEEGEDRFVDLCAAGSPFARASLKSGKTESSSYFRRKEKMFRFLIRRMVKAQSFYWVVL CVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIF EVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQ LFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYT LLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSS SVSSVNSPQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVV EPGRDGLRGPAGNKSKPEGTEATEGADPPRRHHRHRDRDKTSASTPAGGEQDRTDCPKAESTETGAREER ARPRRSHSKEAPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGKEGTAPVLVPKGER RARHRGPRTGPRETENSEEPTRRHRAKHKVPPTLEPPEREVAEKESNVVEGDKETRNHQPKEPRCDLEAI AVTGVGSLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGEATVVPSANTDLE GQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDP VRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGK DINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCT DESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQ GPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRY MPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSLECILKI IAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTF VQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDGTSINRHNNFRTFLQALMLLFRSATGEAWHEIML SCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRV WAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTA LEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRD QTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQ RTQDVLYEARAPLERGHSAEIPVGQPGALAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPA PNASPMKRSISTLAPRPHGTQLCNTVLDRPPPSQVSHHHHHRCHRRRDKKQRSLEKGPSLSVDTEGAPST AAGSGLPHGEGSTGCRRERKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQPKPSLSSHPISPTAA LEPGPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSP GRLSRGLSEHNALLQKEPLSQPLASGSRIGSDPYLGQRLDSEASAHNLPEDTLTFEEAVATNSGRSSRTS YVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001195199
ORF Size	7062 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001195199.1, NP_001182128.1
RefSeq Size	7065 bp
RefSeq ORF	7065 bp
Locus ID	257648
MW	264 kDa

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