Nav3 (NM_001191782) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1306259
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217762 representing NM_001191782 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTCCTTGGGGTTGCCTCAAAACTGAGACAGCCAGCTGTTGGGCCAAAGCCTGTTCACGCTGCTC TTCCGATACCGAATCTTGGCATCAGTGTGTCACAGCGCTGTTCTTCAAGACCTTTGGAATTTGCTACACC GGAGCGCTCAATTCTTTCTTGCCAGCTCACATTAAAATCAACCTGTGAGTTTGGAGAGAAGAAAGCCCTG CAGGGAACAGCCAAGGACATCGAAGACAGCAAGATTTACACCGACTGGGCCAACCACTACCTGGCAAAAT CGGGCCACAAGCGGCTGATCAAGGATTTGCAGCAGGACATTGCGGACGGGGTCCTTCTGGCAGATATTAT ACAGATCATTGCAAATGAGAAAGTGGAAGATATCAATGGATGTCCGAGAAGTCAATCTCAGATGATTGAA AATGTCGATGTCTGCCTTAGTTTTCTAGCAGCCCGAGGAGTAAATGTTCAAGGTCTATCTGCTGAAGAAA TAAGAAATGGAAACCTAAAAGCCATCCTAGGGCTCTTCTTCAGCTTGTCTCGCTACAAGCAGCAGCAGCA TCATCAACAACAGTATTACCAGTCCTTGGTAGAGCTTCAACAGCGAGTGACTCACGCTGCTCCTCAGTCA GAAGCCAGCCAGGCCAAAACCCAGCAAGATATGCAGTCCAGTCTGACAGCCAGATATGCAGCTCAGTCTA AGCACAGTGGAATTGCAACCAGTCAGAAAAAGCCTACTAGGTCAGTTCATGTCTCTAATCTATTGTTTGC TAGTGATATTCACGTGAATGCCGATTCCTCCAAAGGACCTCAACCATCTTCAGGTATAAATGGAAACACA CAGCCTCCCAGCACTTCTGGGCAACCCCCAGCCTCTGCCATCCCTTCCCCGAATGCCAGCAAGCCGTGGC GCAGCAAGTCCATGAATGTCAAGCACAGTGCCACCTCCACCATGCTGACAGTGAAACAGCCAAGCCCAGC CACCTCCCCCACACCATCTTCAGACAGACTGAAGCCCCCTGTCACAGAAGGGGTCAAAAGTGCTCCTTCG GGGCAGAAATCTATGCTTGAAAAATTCAAGCTGGTTAATGCCCGGACAGCATTGCGCCCCCCTCAGGCTA CCAGTTCAGGACCTAATGATAGTGGCAAAGATGATGATGCCTTTTCTGAATCAGGAGAAATGGAAGGTTT TAACAGTGGTCTGAATAGTGGCGGCTCAACAAACAGCAGTCCCAAAGTGTCACCAAAATTAACCCCTCCA AAAGCAGGAAGCAAAAATTTCAGCAATAAAAAGTCTTTGCTGCAGCCAAAAGAAAAGGAGGAAAAGAACA GGGACAAGAATAAGGCCTGTGCTGAGAAGCCAGGAAAAGAAGAGAAGGACCAGATGATGGATGCGACTCC GAAAAAGACCTCAAAAATCGCAAGCTTGATCCCAAAGGGCAGCAAGACCGCAGCAGCCAAGAAAGACAGC TTAATTCCGTCGTCGTCCAGCGGAATTCCAAAGCCAGGCTCAAAGGTGCCCACACCAAAGCAAACTATTT CCCCTGGCAGTGCAGCAAGCAAAGAGTCTGAGAAATTCAGGACTAGCAAGGGAAGCTCTTCACAGGCCTT CCAAAAGGCCATCACTATGGAGAAAACAAGCACGCCCAGCCTCTCTGCTCCTCTGGAGGGGAGGGAAGCT AGCCAAGCATCTCCCTCTAGCTCCTGCGTTCTGCAGGTAGCACACAGCAGTGGACAGAGTCCTGGCAATG GAGCTGTCCATCTTCCTCAGCAGCAACAGCATAGCCATCCTAACACTGCCACGGTGGCTCCATTCATTTA CAGAGCACATTCAGAAAATGAAGGCACCCTTTTACCACCTGCTGACTCCTGTACTAGCCCCACCAAAATG GATTCTTCCTATAGTAAGACAGCTAAGCAATGCCTAGAAGAGCTATCTGGTGAAGACCCTGAAGCCCGGA GAATGCGCACAGTCAAGAACATAGCAGATCTGAGGCAGAACTTGGAAGAGACGATGTCCAGCCTTCGAGG GACCCAGATAAGTCACAGCACCCTGGAGACAACCTTTGATACGACTGTGACAACTGAAGTGAATGGAAGG GCCATCCCCAACCTGACAAGCCGACCTACCCCCATGACCTGGAGACTGGGTCAGGCGTGTCCTCGTCTAC AGGCTGGAGATGCCCCCTCCATGGGCGCTGGATATTCTCGAAGTGGCACCAGCCGGTTCATCCACACGGA CCCCTCCAGGTTTATGTATACAACACCTCTCCGCAGAGCTGCAGTCTCCCGTCTGGGAAACATGTCACAA ATAGACATGAGCGAGAAAGCAAGCAGTGACCTGGATGTGTCTTCTGAAGTGGATGTTGGAGGATACATGA GTGATGGTGATATCCTCGGGAAGAGTCTGAGGGCAGATGATATCAACAGTGGGTACATGACAGACGGTGG GCTCAACCTATACACTAGAAGTCTTAACCGAGTGCCGGACACAGCAACTTCCAGGGATGTCATCCAGAGA GGCGTTCACGATGTGACAGTGGACGCAGACAGCTGGGATGACAGCAGTTCCGTGAGCAGTGGTCTCAGTG ATACACTTGATAACATTAGCACAGATGACCTCAACACCACGTCTTCCATCAGCTCCTACTCCAACATCAC GGTCCCCTCCAGGAAGAACACTCAGCTGAAAACAGACACAGAGAAGCGTTCGACCACAGATGAGACCTGG GATAGTCCGGAGGAGCTGAAGAAAACCGAGGAGGATTTGGACAGCCACGTCGATGGAGGCGCCAAGTGGA AGGGTGTTGCTTCTGGACTTGCTGAAGACTCAGAGAAGACGGGGCAGAAAGCCAGCCTGTCCGTGGCTCA GACAGGCTCCTGGAGGAGAGGCATGTCTGCCCAGGGAGGAACACCAGCGAGGCAGAAAACCAGCACGAGT GCACTCAAGACCCCTGGGAAGACAGATGATGCCAAAGCTTCCGAGAAAGGGAAAACGCCTCTTAAAGGAT CATCCTTGCAAAGGTCTCCTTCAGATGCAGGGAAAAGCAGCGGGGATGAAGGGAAAAAGCCACCCTCAGG CATTGGAAGATCCACAGCCAGCAGTTCTTTTGGATATAAGAAGCCAAGTGGTGTAGGGGCCTCTACCATG ATTACCAGCAGCGGCGCCACCATAACAAGCGGCTCAGCGACACTGGGAAAAATCCCCAAATCTGCTGCCA TTGGTGGGAAGTCCAATGCAGGAAGGAAAACCAGCCTGGATGGGTCCCAGAATCAAGAGGATGTCATGCA TGTGAGCTCTAAGGCCACCCTTCAGTACCGCAGTTTGCCCCGCCCTTCTAAGTCGGGCACCAGCGGTATC CCTGGGAGAGGTGGTCACAGATCCAGCACCAGCAGCATTGATTCCAATGTCAGCAGCAAGTCAGCTGGGG CCACCACCTCTAAACTGAGAGAACCCACTAAGATCGGCTCAGGGCGCTCGAGTCCAGTCACTGTCAACCA AACTGACAAAGAAAAGGAGAAAGTAGCAGTGTCGGATTCAGACAGTGTTTCCTTGTCAGGTTCCCCCAAA TCCAGCCCCACCTCTGCCAGTGCCTGCGGTACTCAAGGGCTCAGACAGCCAGGGTCCAAATATCCAGATA TTGCCTCGCCTACATTCAGAAGGTTGTTCGGTGCCAAGGCAGGTGGCAAATCTGCCTCCGCACCTAATAC TGAGGGGGTGAAATCCTCCTCGGTAGTGCCCAGCCCTAGTACCTCTTTAGCCCGACAAGGCAGTCTGGAG TCACCGTCGTCCGGTACGGGAAGCATGGGCAGTGCTGGTGGGCTGAGCGGCAGCAGCAGCCCTCTCTTCA ATAAACCCTCAGACCTAACTACAGATGTTATAAGCTTAAGTCACTCCTTGGCTTCCAGCCCAGCGTCGGT TCACTCTTTCACATCCGGTGGGCTTGTGTGGGCTGCCAATCTGAGCAGTTCCTCTGCCGGCAGCAAGGAC ACTCCAAGTTACCAGTCCATGACTAGTCTCCATACGAGCTCTGAGTCCATTGATCTGCCCCTCAGCCATC ATGGCTCCCTGTCTGGACTGACCACAGGCACTCACGAGGTGCAGAGCCTGCTCATGAGAACGGGTAGTGT GAGATCTACTCTCTCAGAAAGCATGCAGCTTGACAGAAATACACTACCCAAAAAGGGACTAAGATACACC CCATCATCTCGGCAGGCCAACCAGGAAGAAGGCAAAGAGTGGCTACGTTCACATTCCACTGGCGGGCTGC AGGATACTGGCAGCCAGTCCCCCTTGGTGTCTCCTTCTGCCATGTCATCTTCAGCCACCGGAAAATACCA CTTTTCCAACTTGGTGAGTCCCACCAACCTGTCTCAGTTTAACCTGCCTGGACCCAGCATGATGCGTTCC AACAGCATCCCCGCCCAGGACTCCTCCTTCGACCTCTATGATGATGCCCAGCTTTGCGGAAGTGCCACGT CTCTGGAGGAAAGGCCACGTGCCATCAGCCACTCGGGCTCGTTCAGAGACAGCATGGAGGAAGTTCATGG CTCCTCGCTCTCCTTGGTCTCCAGCACATCCTCTCTTTACTCTACGGCTGAGGAGAAGGCTCATTCAGAG CAAATCCATAAGCTACGGAGAGAACTGGTTGCCTCCCAGGAGAAAGTTGCTACCCTCACATCTCAGCTGT CAGCAAACGCTCACCTTGTAGCAGCTTTTGAAAAGAGTTTAGGGAATATGACTGGCCGTTTACAAAGTCT AACCATGACAGCGGAACAAAAGGAATCTGAACTTATAGAACTACGGGAAACCATTGAAATGCTGAAAGCC CAGAACTCTGCTGCCCAGGCCGCCATTCAGGGTGCGCTGAATGGCCCAGACCACCCTCCCAAAGATCTCC GCATCAGAAGACAGCACTCCTCTGAAAGCGTTTCTAGTATCAACAGTGCCACAAGCCATTCCAGCATTGG CAGTGGCAATGATGCTGACTCCAAGAAAAAGAAGAAGAAAAACTGGGTGAACTCTAGAGGAAGTGAGCTG AGAAGTTCTTTCAAGCAAGCCTTTGGAAAGAAAAAGTCCACCAAGCCACCCTCCTCGCATTCGGACATTG AAGAGCTGACAGACTCGTCCCTACCCGCGTCCCCCAAGCTACCCCACAATGCTGGCGAGAGTGGCTCCTC GTCCATGAAGCCCTCACAGTCAGCCTCAGCGATCTGTGAATGCACAGAGGCTGAGGCCGAGATCATTCTG CAGTTGAAGAGCGAGCTCAGGGAAAAGGAATTAAAATTAACAGATATAAGGTTGGAGGCCCTCAGCTCCG CTCACCATCTCGATCAGATCCGGGAAGCCATGAACCGGATGCAGAATGAGATTGAAATATTGAAGGCTGA AAATGACCGGCTGAAGGCGGAGACCGGAAACACAGCTAAACCTGCCAGGCCCCCCTCTGAGTCCTCAAGC ACAGCCTCCTCTTCTTCTTCTCGCCAGTCCTTAGGGCTTTCACTCAACAATCTGAACATCACAGAGTCTG TTACCTCAGATATTTTGCTAGACGACACTGGTGATGCGACTGGACATAAGGATGGCCGCCCTGTGAAAAT TATAGTATCCATCAGTAAGGGCTACGGTCGAGCCAAGGACCAGAAGTCTCAGGCATATTTGATAGGATCG ATTGGTGTGAGTGGAAAAACTAAATGGGATGTCTTAGATGGTGTCATTAGGCGTCTCTTTAAGGAATATG TGTTCCGAATTGATACATCCTCTAGTCTTGGTCTGAGCTCTGACTGTATTGCCAGCTACTGTGTAGGAGA TTTAATTAGATCTCATAACCTAGAAGTGCCTGAACTGCTACCTTGTGGATACCTGGTTGGAGATAATAAT ATCATCACTGTGAACCTGAAAGGGGTAGAAGAAAATAGTTTGGACAGTTTTGTTTTTGATACGCTGATTC CTAAACCAATTACCCAAAGGTACTTTAATTTGCTGATGGAGCACCACAGAATTATACTCTCAGGACCAAG TGGTACCGGAAAGACCTATTTAGCAAACAAACTTGCTGAATATGTAATAACCAAATCTGGGAGGAAAAAG ACAGAGGATGCAATTGCTACTTTCAATGTGGACCACAAGTCAAGTAAGGAATTGCAGCAGTATCTTGCCA ACCTGGCTGAGCAGTGCAGTGCAGACAATAATGGGGTAGAGCTCCCTGTTGTCATAATTCTGGATAATCT TCACCACGTGGGTTCCTTGAGTGATATCTTCAATGGTTTTCTCAATTGTAAATACAACAAATGTCCTTAT ATTATTGGAACCATGAATCAGGGAGTGTCTTCGTCACCAAATTTAGAACTACATCACAATTTCAGGTGGG TTTTATGTGCAAATCACATGGAACCTGTAAAAGGCTTTTTAGGCAGGTATCTTCGAAGGAAACTGATAGA GATGGAAATTGAAAGGAATATACGTAATAACGACCTAGTCAAAATTATAGATTGGATCCCTAAGACGTGG CATCATCTCAATAGTGTTTTGGAAACACATAGTTCTTCTGATGTCACCATTGGTCCTCGGCTCTTCCTTC CTTGCCCCATGGATGTGGAAGGTTCTAGGGTGTGGTTCATGGATCTTTGGAATTATTCCTTGGTACCTTA TGTTCTGGAGGCAGTGAGGGAAGGCCTACAGATGTATGGTAAACGTGCACCATGGGAAGATCCCTCCAAG TGGGTCCTTGACACATACCCGTGGAGTTCTGCGTCCTTGCCTCAGGAGGGCCCAGCATTGCTGCAGCTGC GACCAGAAGATGTTGGGTACGAGGGCTGCACATCTACAAAGGAAGCCACAACGTCAAAGCACATTCCCCA GACTGACACAGAGGGGGACCCCTTGATGAATATGCTAATGAAGCTCCAAGAAGCAGCCAATTACCCGGGC ACACAGAGCTGTGACAGTGACAGCGTCAGCCACCATGAAGACATTTTGGATACATCTATTGAATCCACCC TC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217762 representing NM_001191782 Red=Cloning site Green=Tags(s) MPVLGVASKLRQPAVGPKPVHAALPIPNLGISVSQRCSSRPLEFATPERSILSCQLTLKSTCEFGEKKAL QGTAKDIEDSKIYTDWANHYLAKSGHKRLIKDLQQDIADGVLLADIIQIIANEKVEDINGCPRSQSQMIE NVDVCLSFLAARGVNVQGLSAEEIRNGNLKAILGLFFSLSRYKQQQHHQQQYYQSLVELQQRVTHAAPQS EASQAKTQQDMQSSLTARYAAQSKHSGIATSQKKPTRSVHVSNLLFASDIHVNADSSKGPQPSSGINGNT QPPSTSGQPPASAIPSPNASKPWRSKSMNVKHSATSTMLTVKQPSPATSPTPSSDRLKPPVTEGVKSAPS GQKSMLEKFKLVNARTALRPPQATSSGPNDSGKDDDAFSESGEMEGFNSGLNSGGSTNSSPKVSPKLTPP KAGSKNFSNKKSLLQPKEKEEKNRDKNKACAEKPGKEEKDQMMDATPKKTSKIASLIPKGSKTAAAKKDS LIPSSSSGIPKPGSKVPTPKQTISPGSAASKESEKFRTSKGSSSQAFQKAITMEKTSTPSLSAPLEGREA SQASPSSSCVLQVAHSSGQSPGNGAVHLPQQQQHSHPNTATVAPFIYRAHSENEGTLLPPADSCTSPTKM DSSYSKTAKQCLEELSGEDPEARRMRTVKNIADLRQNLEETMSSLRGTQISHSTLETTFDTTVTTEVNGR AIPNLTSRPTPMTWRLGQACPRLQAGDAPSMGAGYSRSGTSRFIHTDPSRFMYTTPLRRAAVSRLGNMSQ IDMSEKASSDLDVSSEVDVGGYMSDGDILGKSLRADDINSGYMTDGGLNLYTRSLNRVPDTATSRDVIQR GVHDVTVDADSWDDSSSVSSGLSDTLDNISTDDLNTTSSISSYSNITVPSRKNTQLKTDTEKRSTTDETW DSPEELKKTEEDLDSHVDGGAKWKGVASGLAEDSEKTGQKASLSVAQTGSWRRGMSAQGGTPARQKTSTS ALKTPGKTDDAKASEKGKTPLKGSSLQRSPSDAGKSSGDEGKKPPSGIGRSTASSSFGYKKPSGVGASTM ITSSGATITSGSATLGKIPKSAAIGGKSNAGRKTSLDGSQNQEDVMHVSSKATLQYRSLPRPSKSGTSGI PGRGGHRSSTSSIDSNVSSKSAGATTSKLREPTKIGSGRSSPVTVNQTDKEKEKVAVSDSDSVSLSGSPK SSPTSASACGTQGLRQPGSKYPDIASPTFRRLFGAKAGGKSASAPNTEGVKSSSVVPSPSTSLARQGSLE SPSSGTGSMGSAGGLSGSSSPLFNKPSDLTTDVISLSHSLASSPASVHSFTSGGLVWAANLSSSSAGSKD TPSYQSMTSLHTSSESIDLPLSHHGSLSGLTTGTHEVQSLLMRTGSVRSTLSESMQLDRNTLPKKGLRYT PSSRQANQEEGKEWLRSHSTGGLQDTGSQSPLVSPSAMSSSATGKYHFSNLVSPTNLSQFNLPGPSMMRS NSIPAQDSSFDLYDDAQLCGSATSLEERPRAISHSGSFRDSMEEVHGSSLSLVSSTSSLYSTAEEKAHSE QIHKLRRELVASQEKVATLTSQLSANAHLVAAFEKSLGNMTGRLQSLTMTAEQKESELIELRETIEMLKA QNSAAQAAIQGALNGPDHPPKDLRIRRQHSSESVSSINSATSHSSIGSGNDADSKKKKKKNWVNSRGSEL RSSFKQAFGKKKSTKPPSSHSDIEELTDSSLPASPKLPHNAGESGSSSMKPSQSASAICECTEAEAEIIL QLKSELREKELKLTDIRLEALSSAHHLDQIREAMNRMQNEIEILKAENDRLKAETGNTAKPARPPSESSS TASSSSSRQSLGLSLNNLNITESVTSDILLDDTGDATGHKDGRPVKIIVSISKGYGRAKDQKSQAYLIGS IGVSGKTKWDVLDGVIRRLFKEYVFRIDTSSSLGLSSDCIASYCVGDLIRSHNLEVPELLPCGYLVGDNN IITVNLKGVEENSLDSFVFDTLIPKPITQRYFNLLMEHHRIILSGPSGTGKTYLANKLAEYVITKSGRKK TEDAIATFNVDHKSSKELQQYLANLAEQCSADNNGVELPVVIILDNLHHVGSLSDIFNGFLNCKYNKCPY IIGTMNQGVSSSPNLELHHNFRWVLCANHMEPVKGFLGRYLRRKLIEMEIERNIRNNDLVKIIDWIPKTW HHLNSVLETHSSSDVTIGPRLFLPCPMDVEGSRVWFMDLWNYSLVPYVLEAVREGLQMYGKRAPWEDPSK WVLDTYPWSSASLPQEGPALLQLRPEDVGYEGCTSTKEATTSKHIPQTDTEGDPLMNMLMKLQEAANYPG TQSCDSDSVSHHEDILDTSIESTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191782
ORF Size	7002 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191782.1, NP_001178711.1
RefSeq Size	7586 bp
RefSeq ORF	7005 bp
Locus ID	314814
MW	249.9 kDa

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