Kdm5a (NM_001277177) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Jarid1a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217682 representing NM_001277177 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGTGGGCCCGGGGGGCTACGCGGCGGAGTTCGTGCCGCCGCCCGAGTGCCCGGTCTTTGAGC CCAGTTGGGAGGAGTTCACAGACCCGCTAAGTTTTATCGGTCGCATCCGGCCTCTGGCTGAGAAAACTGG CATCTGCAAAATCCGTCCACCCAAGGATTGGCAACCTCCATTTGCTTGTGAAGTAAAAACCTTCCGATTC ACTCCCAGAGTCCAGCGCCTGAATGAACTTGAGGCAATGACTAGAGTAAGATTGGACTTCTTGGATCAAC TGGCAAAATTTTGGGAACTTCAAGGATCTACATTGAAGATTCCCGTGGTGGAGAGAAAAATCCTGGATTT GTATGCTTTAAGCAAGATTGTTGCCAGCAAAGGAGGTTTTGAAATAGTCACCAAAGAGAAGAAATGGTCT AAAGTGGGTAGCCGTTTGGGATATCTGCCAGGAAAAGGAACTGGGTCTCTTTTGAAGTCACACTATGAAA GAATTCTCTACCCATATGAGCTTTTCCAATCTGGTGTCAGCCTTATGGGTGTACAAATGCCTGATTTAGA TCTTAAGGAAAAAGTTGAAGCTGAGGTTCTCAGTACTGACATCCAGCCTTCCCCAGAGCGGGGAACAAGA ATGAATATCCCACCAAAGAGAACAAGACGAGTTAAATCCCAGTCAGACTCTGGAGAAGTAAATAGAAATA CAGAACTGAAGAAACTTCAGATTTTTGGGGCTGGACCCAAAGTAGTAGGCCTGGCAGTAGGAGCAAAAGA TAAAGAAGATGAGGTCACCCGAAGACGGAAAGTTACCAACAGGTCAGATGCATTTAACATGCAGATGAGA CAACGGAAAGGAACTCTCTCTGTTAACTTTGTCGATCTCTATGTTTGCATGTTTTGTGGTCGAGGAAACA ATGAGGATAAACTGCTTTTGTGTGACGGGTGTGATGACAGCTACCATACATTTTGTCTGCTTCCACCACT TCCTGATGTGCCCAAAGGAGACTGGAGGTGTCCTAAATGTGTTGCTGAGGAATGTAACAAACCTCGAGAA GCCTTTGGATTTGAGCAAGCTATAAGAGAATATACACTTCAAAGCTTCGGAGAGATGGCAGATAATTTTA AGTCTGATTATTTCAATATGCCAGTGCATATGGTTCCTACAGAGCTAGTAGAAAAGGAGTTCTGGCGGCT AGTAAGTAGTATTGAAGAAGACGTCATTGTAGAGTATGGAGCTGATATCTCTTCAAAAGATTTTGGAAGT GGATTTCCTATAAAGGATGGTCAGAGAAAGATGTTACCAGAAGAGGAGGAGTATGCACTTTCTGGTTGGA ATTTGAATAACATGCCTGTTCTGGAGCAGTCTGTTCTTGCTCATATTAATGTGGACATTTCTGGTATGAA GGTGCCGTGGCTCTATGTGGGAATGTGCTTTTCTTCTTTTTGCTGGCATATTGAAGATCACTGGAGTTAT TCAATTAACTACTTGCATTGGGGCGAGCCAAAGACATGGTATGGTGTACCATCTCATGCTGCAGAACAAC TGGAGGAGGTGATGAGGGAGCTGGCCCCTGAGTTATTTGAATCACAGCCTGACCTCCTACATCAGTTAGT AACCATCATGAACCCAAATGTGCTCATGGAGCATGGTGTGCCTGTGTACAGAACTAATCAGTGTGCTGGA GAGTTTGTGGTGACGTTTCCTCGTGCCTATCACTCTGGATTTAACCAGGGATACAACTTTGCTGAAGCCG TGAACTTCTGTACTGCTGACTGGTTGCCCATTGGACGTCAGTGTGTAAATCACTATCGACGGCTGAGGCG CCATTGTGTCTTTTCACATGAGGAACTCATTTTCAAGATGGCAGCAGATCCAGAATGCTTAGATGTGGGG CTGGCTGCCATGGTATGCAAAGAGTTGACTCTCATGACTGAAGAAGAAACACGATTGAGGGAATCGGTTG TTCAAATGGGTGTCGTGATGTCAGAAGAAGAAGTCTTCGAACTTGTTCCTGATGATGAGCGGCAGTGTTC AGCATGCAGAACCACATGTTTTCTCTCAGCTCTCACATGCTCCTGTAATCCAGAGCGACTTGTATGTTTG TATCATCCAACTGATTTGTGCTCCTGCCCCATGCAGAACAAATGTCTTAGGTACCGATACCCACTTGAAG ACCTCCCATCTCTGCTTTATGGTGTCAAAGTGAGAGCACAGTCCTATGACACTTGGGTCAATCGTGTCAC AGAAGCATTATCAGCTAGCTTCAACCATAAGAAAGATTTGATTGAATTGAGAGTAATGCTGGAAGATGCT GAGGATAGGAAATACCCAGAGAATGATCTCTTTCGAAAGCTCAGGGATGCGGTAAAAGAAGCTGAGACCT GTGGTTCTGTGGCTCAGCTGCTTCTGAGCAAAAAACAAAAACACAGGCAGAGCTCAGATAGTGGGAAGAC TCGGACCAAACTGACAGTAGAAGAATTGAAGGCCTTTGTCCAACAACTTGTTAGTCTTCCGTGTGTCATC AGCCAAACTCGACAAGTAAAGAATCTGCTGGATGATGTAGAAGAATTTCATGAACGTGCCCAGGAGGCCA TGATGGATGAAACTCCAGATTCTTCCAAACTCCAAGTGTTAATAGACATGGGCTCTAGTCTCTATGTGGA ACTACCTGAATTGCCCCGACTAAAGCAAGAGCTACAGCAGGCTCGGTGGCTGGATGAAGTAAGACTGACT CTATCAGACCCACAACAAGTCACTTTGGATGTCATGAAGAAATTGATTGACTCTGGGGTAGGCTTGGCAC CTCACCATGCTGTGGAAAAGGCAATGGCTGAACTACAGGAACTTCTTACAGTCTCTGAACGATGGGAAGA GAAGGCGAAGGTCTGCCTGCAGGCAAGACCACGGCACAGTATGGCAAATTTAGAAAATATTGTAAATGAA GCCAAGAACATTCCAGCCTTTCTACCCAATGTGTTGTCCTTAAAAGAAGCCTTACAAAAGGCTCGTGAAT GGACAGCTAAAGTGGAAGCTATTCAGAGCGGCAGCAACTATGCTTACTTGGAACAGCTTGAGAGCTTGTC TGCTAAAGGACGCCCTATCCCTGTGCGCCTCGATGCCTTGCCTCAAGTGGAGTCACAAGTAGCAGCAGCG CGAGCCTGGAGGGAGCGGACGGGGCGGACCTTCCTTAAGAAGAATTCCAGCCATACATTGTTACAGGTGC TAAGTCCACGAACAGACATTGGTGTATATGGCAGTGGCAAAAACAGAAGGAAAAAAGTAAAAGAAATAAT AGAAAAAGAAAAGGAGAAGGACCTAGACCTTGAACCTTTGAGTGACTTAGAAGAAGGACTGGAGGAGACG AGGGACGCAGCCATGGTGGTGGCAGTTTTCAAAGAACGAGAACAAAAGGAGATTGAAGCCATGCATTCCC TCAGAGCAGCCAACCTAGCCAAGATGACCATTGTGGACCGCATAGAAGAAGTAAAGTTTTGTATTTGCCG CAAGACAGCGAGTGGGTTTATGCTACAGTGTGAGCTCTGCAAAGACTGGTTCCATAATGGCTGTGTTCCC CTTCCTAAGTCAGGTTCCCAGAAAAAGGGATCCAGCTGGCAAGCCAAAGACGTAAAATTCCTTTGCCCTC TCTGCATGAGATCCCGGAGGCCGAGGCTAGAGACTATTCTGTCACTCCTGGTATCCCTTCAGAAGTTGCC TGTACGATTGCCTGAAGGAGAAGCACTGCAGTGTTTGACAGAGCGTGCTATGAGTTGGCAAGATAGAGCA CGACAAGCTTTAGCCACAGATGAACTGTCTTCTGCCCTGGCCAAACTCTCTGTGTTAAGCCAGCGTATGG TAGAACAAGCAGCCCGAGAAAAAACTGAAAAGATTATCAGTGCAGAACTCCAGAAAGCAGCCGCCAATCC AGACTTACAGGGACACTTACCTAGTTTCCAGCAGTCTGCTTTTAACCGGGTGGTGAGCAGTGTATCCTCT TCCCCCCACCAAGCGATGGACTATGATGATGAAGAAACAGACTCTGATGAAGATATTCGAGAGACATATG GCTATGACATGAAGGATACAGCCAGTGTAAAGTCCTCTAGCAGTCTGGAACCCAATCTCTTTTGCGATGA AGAGATTCCTATCAAATCGGAGGAAGTGGTGACCCACATGTGGACAGCACCTTCATTCTGTGCAGAGCAT GCATATTCTTCTGCTTCTAAGAGTTGTTCTCAAGGTTCTAGCACTCCAAGAAAGCAGCCTCGGAAGAGCC CTTTGGTTCCCAGAAGTTTGGAGCCTCCAGTATTAGAATTGTCTCCTGGAGCAAAAGCCCAGTTGGAAGA ACTCATGATGGTTGGAGACCTCCTAGAAGTGTCTCTGGATGAGACACAACACATATGGCGGATTTTGCAG GCCACACATCCACCCTCAGAGGACAGATTCTTACATATCATGGAGGATGATAGCGTAGAAGAGAAACCAT TAAAAATGAAAGGAAAGGATTCTTCAGAGAAGAAACGGAAGCGGAAATTAGAAAAGGTAGAACAGTTTTT TGGAGAAGGAAAACAGAAGACCAAGGAGCTAAAGAAAATAGATAAACCTAAAAAGAAGAAATTAAAATTA AATGTGGACAAATCAAAAGAGCTGAATAAATTGGCTAAGAAGTTAGCAAAAGAAGAAGAGCGAAAGAAAA AGAAAGAAAAGGCTGCTGCAGCCAAAGTGGAGCTTGCGAAAGAGAGCACCGAGAAGAAAAGAGAGAGAAA GGTGCTGGATATCCCTTCAAAGTATGACTGGTCGGGCGCAGAGGAGTCTGATGATGAGAATGCTGTATGT GCAGCACAGAACTGCCAAAGGCCGTGTAAGGACAAGGTAGACTGGGTACAATGTGATGGTGGCTGTGATG AGTGGTTTCATCAAGTTTGTGTTGGTGTATCCCCAGAAATGGCTGAAAATGAAGATTACATCTGTATAAA CTGTGCAAAGAAGCAGGGGCCAGATAGCCCAGGCCACGCACCACCTCCTCCCTTCATAATGAGCTACAAA CTACCAATGGAGGATCTTAAAGAGACCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217682 representing NM_001277177 Red=Cloning site Green=Tags(s) MAGVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPLAEKTGICKIRPPKDWQPPFACEVKTFRF TPRVQRLNELEAMTRVRLDFLDQLAKFWELQGSTLKIPVVERKILDLYALSKIVASKGGFEIVTKEKKWS KVGSRLGYLPGKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPDLDLKEKVEAEVLSTDIQPSPERGTR MNIPPKRTRRVKSQSDSGEVNRNTELKKLQIFGAGPKVVGLAVGAKDKEDEVTRRRKVTNRSDAFNMQMR QRKGTLSVNFVDLYVCMFCGRGNNEDKLLLCDGCDDSYHTFCLLPPLPDVPKGDWRCPKCVAEECNKPRE AFGFEQAIREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADISSKDFGS GFPIKDGQRKMLPEEEEYALSGWNLNNMPVLEQSVLAHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSY SINYLHWGEPKTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGVPVYRTNQCAG EFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVNHYRRLRRHCVFSHEELIFKMAADPECLDVG LAAMVCKELTLMTEEETRLRESVVQMGVVMSEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCL YHPTDLCSCPMQNKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVNRVTEALSASFNHKKDLIELRVMLEDA EDRKYPENDLFRKLRDAVKEAETCGSVAQLLLSKKQKHRQSSDSGKTRTKLTVEELKAFVQQLVSLPCVI SQTRQVKNLLDDVEEFHERAQEAMMDETPDSSKLQVLIDMGSSLYVELPELPRLKQELQQARWLDEVRLT LSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLTVSERWEEKAKVCLQARPRHSMANLENIVNE AKNIPAFLPNVLSLKEALQKAREWTAKVEAIQSGSNYAYLEQLESLSAKGRPIPVRLDALPQVESQVAAA RAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRRKKVKEIIEKEKEKDLDLEPLSDLEEGLEET RDAAMVVAVFKEREQKEIEAMHSLRAANLAKMTIVDRIEEVKFCICRKTASGFMLQCELCKDWFHNGCVP LPKSGSQKKGSSWQAKDVKFLCPLCMRSRRPRLETILSLLVSLQKLPVRLPEGEALQCLTERAMSWQDRA RQALATDELSSALAKLSVLSQRMVEQAAREKTEKIISAELQKAAANPDLQGHLPSFQQSAFNRVVSSVSS SPHQAMDYDDEETDSDEDIRETYGYDMKDTASVKSSSSLEPNLFCDEEIPIKSEEVVTHMWTAPSFCAEH AYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQ ATHPPSEDRFLHIMEDDSVEEKPLKMKGKDSSEKKRKRKLEKVEQFFGEGKQKTKELKKIDKPKKKKLKL NVDKSKELNKLAKKLAKEEERKKKKEKAAAAKVELAKESTEKKRERKVLDIPSKYDWSGAEESDDENAVC AAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMAENEDYICINCAKKQGPDSPGHAPPPPFIMSYK LPMEDLKETS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277177
ORF Size	5070 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277177.1, NP_001264106.1
RefSeq Size	10937 bp
RefSeq ORF	5073 bp
Locus ID	312678
MW	192 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.