Alk (NM_001169101) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217670 representing NM_001169101 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCCCTTGGGTTCCTGTGGCTGCTGCCGCCGCTGCTTTTGACAGCAGCCTCGTACTCCGGAGCTG CAACCGATCAGCGCGCGGGTTCTCCAGCCTCAGGGCCGCCTCTGCAGCCCCGGGAGCCGCTCAGCTATTC GCGCCTGCAGAGGAAGAGTCTGGCGGTGGACTTCGTGGTGCCCTCACTCTTCCGCGTCTATGCCCGAGAC CTGCTGCTGCCGCAGCCACCGTCCCCCTCGGAGCCCGGGGCTGGCGGGCTGGAGGCGCGGGGATCACTGG CTCTGGACTGCGACCCTCTGCTCAGGTTGCTGGGGCCATCGCCTGGAATCTCCTGGGCAGAGGGAGCCAG TTCTCCTAGTCCGGAGGCGGCTCCGACGCTGTCCAGGGTGCTGAAGGGCGGCTCGGTGCGCAAGCTCAGG CGTGCCAAACAGCTGGTGCTGGAGCTGGGCGAGGAGACGATCCTTGAAGGCTGCATTGGTCCCCCAGAGG AGGCGGCGGCTGTGGGGATACTCCAGTTCAACCTTAGCGAGCTGTTCAGCTGGTGGATTCTACACGGCGA AGGGCGGCTGAGGATCCGCCTGATGCCTGAGAAGAAGGCATCGGAAGTGGGCAGGGAGGGAAGGCTATCC ACTGCGATCCGAGCCTCCCAGCCCCGCCTTCTCTTCCAGATCTTCGGGACCGGTCACAGCTCCTTGGAAT CACCCTCAGAAATGCCTTCTCCTCCTGGTAACTTTCTATGGAATCTCACCTGGACAATGAAAGACTCCTT CCCTTTCCTTTCCCACCGCAGTCGATATGGTCTGGAGTGCAGCTTCGATTTTCCCTGTGAGCTAGAATAC TCCCCTCCCCTCCACACCCATGGGAACCAGAGCTGGTCCTGGCGCCGTGTGCCCTCTGAGGAGGCCTCGA GGATGAACTTGTTGGATGGGCCAGAGGCAGAGCATTTGAAAGAGATGCCCAGAGGCTCCTTCCTCCTCCT GAACACCTCTGCAGATTCCAAGCATACCATCCTGAGCCCCTGGATGAGGAGCAGTAGTGAGCACTGCACG CTGGCTGTCTCAGTGCACAGACATCTACAGCCTTCTGGGAGATATGTTGCCCAGCTCCTACCCCACAATG AAGCTGGAAGAGAGATTCTTCTGGTGCCTACCCCGGGGAAACATGGCTGGACAGTGCTGCATGGGAGAGT CGGGCGCCCAGAGAACCCATTCCGAGTGGCCCTGGAATACATCTCGAGTGGAAACCGGAGCTTGTCTGCG GTGGATTTCTTTGCCCTGAAGAACTGCAGTGAAGGAACATCCCCAGGCTCCAAGATGGCCTTGCAGAGTT CCTTCACTTGTTGGAACGGGACAGTCCTCCAGCTCGGGCAAGCCTGTGACTTCCACCAGAACTGTGCCCA AGGAGAAGATGAGGGTCAGCTATGCAGTAAACTTCCTGCTGGGTTTTACTGTAACTTCGAGGATGGCTTC TGTGGCTGGACCCAAAGTCCACTCTCACCCCGTGTGCCCCGATGGCAAGTGAAGACCCTGAAAGATACCC ATTCCCAGGGCCACCAAGGCCATGCCCTGTTGCTCAGCACCACTGACGACCCCACTTCAGAAAGTGCAAC AGTGACCAGTGCTACATTCCCCGCACCAATGAAGAGCTCTCCTTGTGAGCTCCGGATGTCCTGGCTCATC CGTGGGGTTCTGAAAGGAAATGTATCTTTGGTGCTGGTGGAGAACAAAACTGGAAAGGAGCAAAGCCGGA CCGTCTGGCATGTTGCCACCAATGAAGGCCTAAGCCTGTGGCAGTGGACAGTGTTGTCGCTCCTCGATGT GACTGACAGGTTCTGGCTGCAAATAGTCACATGGTGGGGCCCAGGATCCAGGGCAACCGTGGCATTTGAC AACATTTCCATCAGCCTCGACTGCTACCTCACCATCAGTGGGGAGGAGAAAATGTCCCTGAATGCAGTAC CCAAATCCAGAAATCTGTTTGAGAAAAACCCAAACAAGGAGCCAAAACCCTGGGCAAACATATCAGGACC AACTCCCATCTTCGACCCTACAGTTCACTGGCTGTTCACCACATGTGGGGCCAGTGGCCCTCATGGCCCC ACACAGGCACAGTGCAACAACGCCTACCAGAACTCCAACCTGAGCGTGGTGGTGGGAAGTGAAGGGCCCT TGAGGGGCATCCAGATTTGGAAAGTGCCAGCTACTGACACCTACAGTATCTCAGGCTATGGAGCAGCTGG CGGAAAAGGTGGGAAGAACACCATGATGCGGTCCCATGGCGTGTCTGTCCTGGGCATCTTCAATCTGGAG AAAGATGACACGCTCTACATCCTTGTCGGTCAACAAGGGGAAGATGCCTGTCCCAGGGCAAACCAACTAA TCCAGAAAGTATGTGTCGGCGAGAACAATGTGATAGAAGAAGAGATCCGAGTGAACAGAAGCGTTCACGA GTGGGCAGGAGGAGGAGGAGGTGGGGGTGGAGCCACCTACGTGTTTAAGATGAAAGACGGCGTGCCCGTG CCCCTGATTATTGCGGCCGGCGGTGGTGGCAGGGCCTATGGGGCCAAGACAGAAACGTTCCACCCAGAGA GACTGGAGAATAACTCCTCAGTTCTAGGGCTGAACGGCAATTCCGGAGCCGCAGGTGGTGGAGGCGGCTG GAATGATAACACTTCCTTGCTCTGGGCCGGAAAGTCTTTGTTGGAGGGCGCCGCCGGAGGACATTCCTGC CCCCAGGCCATGAAGAAGTGGGGGTGGGAGACAAGAGGGGGTTTCGGAGGGGGTGGAGGGGGGTGCTCCT CAGGTGGAGGAGGCGGAGGATATATAGGTGGCAACGCAGCATCAAACAATGACCCCGAAATGGATGGGGA AGATGGGGTTTCCTTCATCAGTCCATTGGGTATCCTGTATACCCCGGCCTTAAAAGTGATGGAGGGCCAT GGGGAAGTGAATATCAAGCACTATCTAAACTGCAGCCACTGTGAGGTAGACGAATGTCACATGGACCCCG AGAGCCACAAAGTCATCTGCTTCTGTGACCATGGGACCGTGCTGGCTGACGATGGTGTCTCCTGCATTGT GTCGCCCACCCCGGAACCCCACCTGCCGCTCTCATTGATCCTCTCCGTCGTGACCTCTGCCCTGGTGGCC GCTCTCGTTCTGGCATTCTCCGGCATCATGATTGTGTACCGCCGGAAGCACCAGGAGTTGCAAGCTATGC AGATGGAGCTGCAGAGCCCTGAGTATAAGCTGAGCAAGCTACGGACCTCGACCATCATGACAGACTACAA CCCCAACTACTGCTTTGCTGGCAAGACTTCATCCATCAGTGACCTGAAGGAGGTGCCTCGGAAAAACATC ACCCTCATCCGGGGCCTAGGCCATGGCGCATTTGGGGAGGTGTATGAAGGCCAGGTATCTGGAATGCCCA ATGACCCAAGCCCTCTACAAGTGGCTGTAAAGACGCTGCCGGAAGTGTGTTCAGAGCAAGATGAGCTGGA CTTCCTCATGGAAGCTCTGATCATCAGTAAATTCAACCACCAGAACATTGTCCGCTGCATCGGGGTGAGT CTGCAAGCCCTGCCCCGCTTCATCCTGCTGGAGCTCATGGCTGGTGGAGACCTCAAGTCCTTCCTCAGGG AGACACGTCCTCGCCCGAACCAGCCCACCTCCCTGGCCATGTTGGATCTTCTGCACGTAGCTCGGGACAT TGCCTGTGGCTGTCAGTACCTGGAGGAAAATCACTTTATCCACCGCGATATTGCTGCTAGAAACTGTCTG CTGACCTGTCCGGGAGCTGGGAGGATAGCGAAGATCGGAGACTTCGGGATGGCCCGAGATATCTACAGGG CCAGCTACTACAGAAAGGGAGGCTGTGCCATGCTGCCAGTGAAGTGGATGCCCCCGGAGGCCTTCATGGA AGGAATATTTACTTCTAAAACAGATACATGGTCTTTTGGAGTGTTGCTGTGGGAAATATTTTCTCTTGGA TATATGCCATACCCCAGCAAGAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGTGGAGGACGGATGGACC CACCTAAGAACTGCCCCGGACCTGTATACCGGATAATGACTCAGTGCTGGCAGCATCAGCCTGAGGACAG ACCCAACTTTGCCATCATTCTGGAGAGGATCGAATACTGCACCCAGGACCCCGATGTCATCAATACAGCT CTGCCCATCGAATATGGCCCCGTAGTAGAGGAGGAGGAGAAAGTGCCCATGCGCCCCAAAGACCCTGAGG GGATGCCTCCTCTGCTGGTGTCTCCCCAGTCTGCGAAGCACGAGGAGGCGTCCTCAGCCCCCCAACCCTC AGCCCTGGCAGCGCCAGGCCCGCTGGTGAAGAAGCCCTCGGGTGCAGGCGCGGGAGCGGGTGCCGGTCCG GTGCCCCGAGGTGCAGCCGATCGGGGCCACGTGAACATGGCGTTCTCTCAGCCCAACCCTCCCCCAGAGC TGCACAAAGGCCCGGGCTCCAGAAACAAGCCGACCAGCCTGTGGAACCCCACCTACGGCTCGTGGTTCAC CGAGAAGCCAGCCAAAAAGACCCATCCCCCACCGGGCGCGGAGCCGCAGGCCCGGGCAGGAGCGGCGGAG GGTGGCTGGACCGGGCCGGGTGCGGGGCCCCGCAGAGCCGAGGCTGCGCTGCTGCTCGAGCCGTCGGCGC TCAGCGCCACCATGAAGGAGGTGCCCCTGTTCAGGCTGCGCCACTTCCCCTGCGGCAACGTCAACTATGG TTACCAGCAACAGGGTCTCCCCTTGGAAGCCACTGCTGCCCCGGGGGACACCGTGCTGAAAAGCAAGACT AAGGTCACCCAGCCAGGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217670 representing NM_001169101 Red=Cloning site Green=Tags(s) MGALGFLWLLPPLLLTAASYSGAATDQRAGSPASGPPLQPREPLSYSRLQRKSLAVDFVVPSLFRVYARD LLLPQPPSPSEPGAGGLEARGSLALDCDPLLRLLGPSPGISWAEGASSPSPEAAPTLSRVLKGGSVRKLR RAKQLVLELGEETILEGCIGPPEEAAAVGILQFNLSELFSWWILHGEGRLRIRLMPEKKASEVGREGRLS TAIRASQPRLLFQIFGTGHSSLESPSEMPSPPGNFLWNLTWTMKDSFPFLSHRSRYGLECSFDFPCELEY SPPLHTHGNQSWSWRRVPSEEASRMNLLDGPEAEHLKEMPRGSFLLLNTSADSKHTILSPWMRSSSEHCT LAVSVHRHLQPSGRYVAQLLPHNEAGREILLVPTPGKHGWTVLHGRVGRPENPFRVALEYISSGNRSLSA VDFFALKNCSEGTSPGSKMALQSSFTCWNGTVLQLGQACDFHQNCAQGEDEGQLCSKLPAGFYCNFEDGF CGWTQSPLSPRVPRWQVKTLKDTHSQGHQGHALLLSTTDDPTSESATVTSATFPAPMKSSPCELRMSWLI RGVLKGNVSLVLVENKTGKEQSRTVWHVATNEGLSLWQWTVLSLLDVTDRFWLQIVTWWGPGSRATVAFD NISISLDCYLTISGEEKMSLNAVPKSRNLFEKNPNKEPKPWANISGPTPIFDPTVHWLFTTCGASGPHGP TQAQCNNAYQNSNLSVVVGSEGPLRGIQIWKVPATDTYSISGYGAAGGKGGKNTMMRSHGVSVLGIFNLE KDDTLYILVGQQGEDACPRANQLIQKVCVGENNVIEEEIRVNRSVHEWAGGGGGGGGATYVFKMKDGVPV PLIIAAGGGGRAYGAKTETFHPERLENNSSVLGLNGNSGAAGGGGGWNDNTSLLWAGKSLLEGAAGGHSC PQAMKKWGWETRGGFGGGGGGCSSGGGGGGYIGGNAASNNDPEMDGEDGVSFISPLGILYTPALKVMEGH GEVNIKHYLNCSHCEVDECHMDPESHKVICFCDHGTVLADDGVSCIVSPTPEPHLPLSLILSVVTSALVA ALVLAFSGIMIVYRRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNI TLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQNIVRCIGVS LQALPRFILLELMAGGDLKSFLRETRPRPNQPTSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCL LTCPGAGRIAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLG YMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTA LPIEYGPVVEEEEKVPMRPKDPEGMPPLLVSPQSAKHEEASSAPQPSALAAPGPLVKKPSGAGAGAGAGP VPRGAADRGHVNMAFSQPNPPPELHKGPGSRNKPTSLWNPTYGSWFTEKPAKKTHPPPGAEPQARAGAAE GGWTGPGAGPRRAEAALLLEPSALSATMKEVPLFRLRHFPCGNVNYGYQQQGLPLEATAAPGDTVLKSKT KVTQPGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001169101
ORF Size	4851 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001169101.2, NP_001162572.1
RefSeq Size	6372 bp
RefSeq ORF	4854 bp
Locus ID	266802
MW	174.8 kDa
Gene Summary	human homolog induces neuronal differentiation; may act as a receptor tyrosine kinase in the mitogen-activated protein kinase cascade [RGD, Feb 2006]

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