Gpr158 (NM_001170326) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217544 representing NM_001170326 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCCATGGCTTACTCTTTACTCCTCTGCCTCCTGCTCGCTCATCTGGGATTGGGAGAGGTTGGTG CCAGCCTCGACCCTTCAGAACGGCCAGATTCTTCTCGAGAGAGGACTTCGAGGGGGAAGCAGCACGGTCA GCAGCTGCCGCGAGCCTCTGCCCCGGACCCCTCTATTCCCTGGAGCCGCTCCACAGACGGCACCATCTTG GCACAAAAACTAGCCGAGGAGGTGCCCATGGACGTGGCCTCTTACCTCTATACTGGGGACTTCCACCAGC TGAAGAGAGCCAACTGCTCAGGCCGCTATGAGTTGGCGGGCCTGCCGGGGAAGTCACCGTCCCTAGCCAG CTCCCATCCTTCCTTGCACGGGGCGCTGGACACTCTGACACACGCCACCAACTTCCTCAACATGATGTTG CAGAGCAATAAGTCTCGGGAGCAGACCGTGCAGGATGACCTGCAGTGGTACCAGGCTTTGGTGCGAAGTC TCCTGGAGGGAGAGCCCAGCATCTCCCGGGCGGCCATCACCTTCAGCACCGAGTCGCTGTCCACGCCCGC CCCGCAAGTCTTCCTCCAGGCCACCCGTGAGGAGAGTCGCATCCTGCTGCAGGACCTGTCCTCCTCCGCT CACCACCTGGCTAACGCCACTCTGGAGACCGAGTGGTTCCACGGGCTTCGGCGAAAGTGGAGGCCCCACT TGCACCGCCGCGGCTCCAATCAGGGGCCACGGGGCCTGGGCCACAGCTGGCGGCGCAGGGATGGGCTTGG CGGGGACAGGAGCCATGTCAAGTGGTCTCCACCTTTTCTGGAGTGCGAGAATGGGAGTTACAAGCCCGGG TGGCTGGTCACTCTCTCTGCTGCCTTCTACGGGCTTCAACCTAACCTGGTCCCGGAATTCAGGGGCGTGA TGAAAGTTGATATAAATCTTCAGAAAGTGGACATTGACCAATGTTCGAGTGATGGCTGGTTTTCAGGAAC CCACAAATGTCACCTTAACAACTCAGAGTGTATGCCAATCAAAGGCCTAGGATTCGTGCTCGGAGCCTAT CAGTGCGTTTGCAAAGCAGGATTCTATCATCCCCGTGTCTTCTCAGTGAACAACTTTCAGAGAAGAGGTC CAGATCACCATTTTTCAGGAAGCACAAAGGATGTGTCAGAAGAAGCCCACGTCTGCCTGCCCTGCCGAGA AGGCTGCCCCTTCTGTGCTGATGACCGCCCATGTTTTGTCCAGGAGGATAAGTACCTGCGTCTCGCCATC ATCTCCTTCCAAGCCCTGTGTATGCTGCTGGACTTTGTCAGCATGCTGGTGGTCTACCACTTCCGCAAAG CAAAGAGCATCAGGGCATCTGGCCTCATCTTGTTGGAAACAATACTCTTTGGCTCTCTGCTTCTTTACTT TCCGGTTGTTATTCTGTACTTTGAGCCAAGTACATTTCGTTGTATTCTCCTGAGGTGGGTTCGTCTTCTC GGTTTTGCGACAGTTTATGGAACTGTGACCCTCAAGCTTCACAGGGTTTTGAAGGTATTCCTTTCAAGAA CAGCACAAAGAATCCCATATATGACTGGCGGTCGGGTCATGAGGATGCTGGCAGTAATAGTCTTGGTAGT GTTTTGGTTTCTGGTTGGCTGGACTTCTTCTATGTGCCAGAACTTGGAGCGGGATATCTTGCTTGTTGGC CAAGGGCAAACATCTGATAACCTCACCTTCAATATGTGCCTCATTGACCGCTGGGATTACATGACAGCAG TTGCTGAATTTTTATTCCTCCTGTGGGGCATTTATCTCTGCTATGCAGTGCGGACTGTGCCGTCAGCATT CCATGAGCCACGCTACATGGCTGTCGCAGTGCACAACGAGCTCATCATCACTGCTATATTCCACACAATC AGATTCGTGCTTGCTTCGAGGCTTCAGCCTGACTGGATGCTGATGCTGTACTTTGCACACACTCACTTGA CTGTGACAGTCACCATCGGGCTGCTTCTGATTCCAAAGTTTTCACACTCAAGCAATAATCCCCGTGATGA CATCGCCACAGAAGCGTATGAAGATGAGTTGGACATGGGTCGTTCTGGATCCTACCTGAACAGCAGCATC AATTCAGCCTGGAGCGAGCACAGTCTAGATCCAGAAGACATTCGGGATGAGCTGAAGAAACTCTATGCCC AGTTAGAGATATACAAACGTAAGAAGATGATCACAAACAACCCCCACCTCCAGAAAAAGCGGTGCTCCAA GAAGGGCTTAGGCCGGTCCATCATGAGGCGTATCACTGAGATCCCAGAGACGGTCAGCAGGCAGTGCTCC AAAGAGGACAAGGAGGGCACAGATCACAGTGCAGCCAAAGGGACAGGCCTTGTCCGGAAGAACCCTACTG AGTCCTCAGGGAACACAGGGAGACCCAAGGAGGAGTCCCTGAAAAACCGGGTCTTTTCTCTCAAGAAATC CCATAGCACATATGACCATGTAAGAGACCAAACTGATGAATCCAGCAGCCTGCCCACAGAGAGCCAGGAG GAGGAGGTCACGGAAAATTCAACCTTGGAGTCGCTGTCAAGTAAAAAACTGACACAAAAAGTCAAAGAAG ATAGCGAGGCCGAGTCCACAGAGTCAGTGCCCTTGGTCTGCAAGTCAGCCAGTGCTCACAACCTCAGCTC TGAAAAGAAGCCCGGGCACCCACGCACATCTATGTTACAGAAGTCGCTCAGTGTCATTGCAAGTGCCAAG GAGAAGACACTAGGGCTGGCTGGTAAAACCCAGACTTTAGTTATGGAAGACCGAGCTAAGTCCCAGAAAC CTCAGCCAAAAGATAGAGAGACAAACAGGAAGTACTCAAATTCCGATAACACAGAGACTAAAGATTCTGG CTGCCCAAACTCTAATCACACAGAGGAGCTAAGAAAGCCCCAGAAATCTGGGATTATGAAACAGCAGAGG GTCAACCTCCCCACTGCCAATCCTGACGCGAGCTCCAGCACCACCCAGATAAAGGACAATTTTGATATTG GAGAAGTGTGCCCTTGGGAGGTTTATGACCTGACCCCCGGTCCTGTGCCTTCAGAACCAAAAGCTCAAAA ACACGTATCAATTGCAGCTTCGGAAGTGGAACAAAACCCAGCTTCTTTTTCGAAGGAGAAGTCCCACCAC AAGCCTAAGGCAGCTGAAGGTCTCTACCAAGCCAATCACAAGAGCATAGACAAGACAGAGGTGTGCCCCT GGGAGAGCCACGGTCAGTCCCCTTTGGAAGATGAGAATCGTCTGATTTCTAAGACTCCAGTTCTCCCAGG GAGAGCCAGAGAAGAGAATGGAAGTCAGTTGTACACAACCAATATGTGTGCTGGGCAATATGAAGAACTG CCCCCCAAAGCCGTAGCATCAAAAGTAGAGAATGAAAATCTCAACCAAATGGGAGACCAGGAGAAACAGA CATCTTCTTCTGTGGATATCATCCCTGGCTCCTGTATCTCGAGTAATAACTCCCCTCAACCCTTAACGTC ACGAGCCGAAGTGTGTCCCTGGGAGTTCGAGCCCCTAGAACAACCAAATGCTGAAAGAATCGTAGCTTTA CCCGCCTCCTCTGCTTTAAGTGCAAGTAAGATACCCGGGCCACGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217544 representing NM_001170326 Red=Cloning site Green=Tags(s) MGAMAYSLLLCLLLAHLGLGEVGASLDPSERPDSSRERTSRGKQHGQQLPRASAPDPSIPWSRSTDGTIL AQKLAEEVPMDVASYLYTGDFHQLKRANCSGRYELAGLPGKSPSLASSHPSLHGALDTLTHATNFLNMML QSNKSREQTVQDDLQWYQALVRSLLEGEPSISRAAITFSTESLSTPAPQVFLQATREESRILLQDLSSSA HHLANATLETEWFHGLRRKWRPHLHRRGSNQGPRGLGHSWRRRDGLGGDRSHVKWSPPFLECENGSYKPG WLVTLSAAFYGLQPNLVPEFRGVMKVDINLQKVDIDQCSSDGWFSGTHKCHLNNSECMPIKGLGFVLGAY QCVCKAGFYHPRVFSVNNFQRRGPDHHFSGSTKDVSEEAHVCLPCREGCPFCADDRPCFVQEDKYLRLAI ISFQALCMLLDFVSMLVVYHFRKAKSIRASGLILLETILFGSLLLYFPVVILYFEPSTFRCILLRWVRLL GFATVYGTVTLKLHRVLKVFLSRTAQRIPYMTGGRVMRMLAVIVLVVFWFLVGWTSSMCQNLERDILLVG QGQTSDNLTFNMCLIDRWDYMTAVAEFLFLLWGIYLCYAVRTVPSAFHEPRYMAVAVHNELIITAIFHTI RFVLASRLQPDWMLMLYFAHTHLTVTVTIGLLLIPKFSHSSNNPRDDIATEAYEDELDMGRSGSYLNSSI NSAWSEHSLDPEDIRDELKKLYAQLEIYKRKKMITNNPHLQKKRCSKKGLGRSIMRRITEIPETVSRQCS KEDKEGTDHSAAKGTGLVRKNPTESSGNTGRPKEESLKNRVFSLKKSHSTYDHVRDQTDESSSLPTESQE EEVTENSTLESLSSKKLTQKVKEDSEAESTESVPLVCKSASAHNLSSEKKPGHPRTSMLQKSLSVIASAK EKTLGLAGKTQTLVMEDRAKSQKPQPKDRETNRKYSNSDNTETKDSGCPNSNHTEELRKPQKSGIMKQQR VNLPTANPDASSSTTQIKDNFDIGEVCPWEVYDLTPGPVPSEPKAQKHVSIAASEVEQNPASFSKEKSHH KPKAAEGLYQANHKSIDKTEVCPWESHGQSPLEDENRLISKTPVLPGRAREENGSQLYTTNMCAGQYEEL PPKAVASKVENENLNQMGDQEKQTSSSVDIIPGSCISSNNSPQPLTSRAEVCPWEFEPLEQPNAERIVAL PASSALSASKIPGPRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170326
ORF Size	3618 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170326.1, NP_001163797.1
RefSeq Size	7200 bp
RefSeq ORF	3621 bp
Locus ID	291352
MW	134.8 kDa

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