Ulk2 (NM_001191645) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217431 representing NM_001191645 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGGTGGGCGACTTCGAGTACAGCAAGCGGGACCTCGTGGGACACGGGGCCTTCGCTGTGGTCT TCCGGGGGCGGCACCGCCAGAAAACTGATTGGGAGGTGGCTATTAAAAGTATTAATAAAAAGAACTTGTC AAAGTCACAAATTCTGCTTGGAAAGGAAATAAAAATCTTAAAGGAACTTCAGCATGAAAATATCGTAGCA CTCTATGATGTTCAGGAATTACCCAACTCTGTCTTTCTGGTGATGGAGTATTGCAATGGTGGAGATCTGG CAGATTATTTGCAAGCTAAAGGAACTCTGAGTGAAGATACTATCAGAGTGTTTCTTCATCAGATTGCAGC AGCCATGCGAATCCTGCACAGCAAAGGGATAATCCACAGGGATCTCAAACCACAGAACATCTTGTTGTCT TACGCCAGTCGAAGGAAGTCGAATGTCAGTGGTATTCGCATCAAAATAGCTGACTTTGGTTTTGCCCGTT ACCTACATAGTAACACAATGGCAGCGACACTGTGTGGATCCCCAATGTACATGGCTCCTGAGGTTATCAT GTCTCAACATTATGATGCTAAGGCAGATTTATGGAGCATAGGAACGGTGATCTATCAATGCCTAGTTGGA AAACCACCTTTTCAGGCTAACAGTCCTCAAGACCTAAGGATGTTTTATGAAAAAAACAGGAGCTTAATGC CTAGTATTCCCAGAGAAACATCACCTTATTTGGCTAATCTCCTTTTGGGCTTGCTTCAAAGAAATCAGAA GGACAGAATGGACTTTGAAGCGTTTTTCAGTCATCCTTTCCTTGAGCAAGTTCCAGTTAGAAAATCTTGC CCAGTCCCAGTGCCTGTGTACTCCGGCCCTGGCCCTGGAAGCTCCTGCAGCAGCTCGCCACCTTGTCGCT TTGCTTCTCCACCATCCCTTCCAGATATGCAGCATATTCAGGAAGAAAACTTATCCTCCCCACCATTGGG TCCTCCCAACTATCTACAGGTGTCCAAAGATTCTGCCAGTAATAGTAGCAAGAACTCTTCTTGTGACACG GATGACTTTGTTTTGGTTCCACACAACATCTCGTCAGACCACTCATATGATATGCCAATGGGGACTGCTG GCAGACGGGCTTCAAATGAGTTCTTTGTGTGTGGAGGGCAGTGTCAACCTACTGTGTCACCTCACAGCGA AACAGCCCCAATTCCAGTTCCTACTCAAGTAAGGAATTATCAGCGCATAGAACAGAATCTTATATCCACT GCCAGCTCTGGTACAAATCCACATAGTTCTCCAAGATCTGCAGTGGTACGAAGGTCTAATACCAGCCCCA TGGGCTTCCTCCGGGTTGGGTCCTGCTCCCCTGTACCAGCAGACACAGTGCAGACGGGAGGACGAAGACT CTCGACCGGCTCTTCCAGGCCTTACTCACCATCCCCTTTGGTTGGTACTATTCCTGAACAGTTTAGTCAG TGCTGCTGTGGACATCCTCAGGGCCATGAAGCCAGGAGTAGACACTCCTCAGGTTCTCCAGTGCCACAGA CCCAGTCACCACAGTCACTCTTACTGGGTGCTAGACTGCAGAGTGCACCCACCCTCACCGATATCTATCA GAACAAGCAGAAGCTCAGAAAGCAGCACTCTGACCCTGTGTGTCCATCCCTTGCCGGGGCTGGATATAGT TACTCACCTCAGCCGAGTCGGCCTGGCAGCCTTGGAACCTCTCCCACCAAGCATATGGGGTCCTCTCCAC GGAGTTCTGACTGGTTCTTTAAAACTCCTTTACCAACAATCATTGGCTCTCCTACTAAGACTACAGCTCC TTTCAAAATCCCTAAAACACAAGCATCTTCTAACCTGTTAGCCTTGGTTACTCGTCATGGACCTGCTGAA AGCCAGTCCAAAGATGGAAATGACCCTCGTCAGTGTTCACATTGCCTCTTAGTACAAGGAAGTGAGAGGC ATCGATCTGAGCAGCAGAGCAAGGCGGTATTTGGCAGATCTGTCAGTACCGGGAAGTTGTCAGAACAACA GCACGTAAAGGCACCTTTAGGTGGACACCAGGGCAGCACAGATAGTTTAAACACAGAACGACCAATGGAT GTAGCTCCAGCAGGAGCCTGTGGTGTTGTGTTGGCATTGCCAGCAGGAACAGCAGCAAGCGCCAGAGCTG TCCTCTTCACCGTGGGGTCTCCTCCACACAGTGCCACAGCCCCTACTTGTACTCACATGGTCCTTCGAAC AAGAACCACCTCAGTGGGGTCCAACAGCTCAGGAGGCTCCTTGTGTTCTGCGAGTGGCCGAGTGTGTGTG GGCTCCCCTCCTGCGCCAGGGTTCGGCTCCTCCCCACCAGGAGCAGAGGCAGCTCCCAGCCTAAGATACG TGCCTTACGGTGCTTCACCACCCAGCCTAGAGGGTCTCATCACCTTTGAAGCCCCTGAACTACCAGAGGA GACACTGATGGAGCGAGAACACACAGACACCTTACGTCATCTGAACATGATGCTGATGTTTACTGAGTGT GTGCTGGACCTGACAGCAGTGAGGGGTGGGAACCCTGAGCTGTGCACATCTGCTGTGTCCCTGTATCAGA TTCAGGAGAGTGTAGTTGTGGACCAGATAAGCCAGCTAAGCAAAGATTGGGGGCGGGTAGAGCAGCTGGT GTTGTACATGAAGGCGGCACAGCTGCTGGCGGCTTCCCTGCATCTCGCCAAAGCTCAGATCAAGTCCGGG AAGCTGAGCCCATCCACGGCTGTGAAACAAGTTGTTAAAAATCTGAATGAAAGATATAAATTCTGCATCA CCATGTGCAAGAAACTTACAGAAAAGCTGAATCGCTTCTTCTCTGATAAACAGAGATTTATTGATGAAAT CAACAGTGTGACTGCAGAGAAACTCATCTATAATTGTGCTGTGGAAATGGTTCAGTCTGCAGCCCTGGAT GAGATGTTCCAGCAGACTGAAGACATCGTTCAGCGATACCACAAGGCAGCCCTTCTTTTGGAAGGCTTAA GTAAGATCCTGCAGGACCCTGCAGATGCTGAGAGTGTGCATAAGTATAAATGTAGTATTGAAAGAAGATT GTCAGCGCTCTGCTGTAGCACTGCAGCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217431 representing NM_001191645 Red=Cloning site Green=Tags(s) MEVVGDFEYSKRDLVGHGAFAVVFRGRHRQKTDWEVAIKSINKKNLSKSQILLGKEIKILKELQHENIVA LYDVQELPNSVFLVMEYCNGGDLADYLQAKGTLSEDTIRVFLHQIAAAMRILHSKGIIHRDLKPQNILLS YASRRKSNVSGIRIKIADFGFARYLHSNTMAATLCGSPMYMAPEVIMSQHYDAKADLWSIGTVIYQCLVG KPPFQANSPQDLRMFYEKNRSLMPSIPRETSPYLANLLLGLLQRNQKDRMDFEAFFSHPFLEQVPVRKSC PVPVPVYSGPGPGSSCSSSPPCRFASPPSLPDMQHIQEENLSSPPLGPPNYLQVSKDSASNSSKNSSCDT DDFVLVPHNISSDHSYDMPMGTAGRRASNEFFVCGGQCQPTVSPHSETAPIPVPTQVRNYQRIEQNLIST ASSGTNPHSSPRSAVVRRSNTSPMGFLRVGSCSPVPADTVQTGGRRLSTGSSRPYSPSPLVGTIPEQFSQ CCCGHPQGHEARSRHSSGSPVPQTQSPQSLLLGARLQSAPTLTDIYQNKQKLRKQHSDPVCPSLAGAGYS YSPQPSRPGSLGTSPTKHMGSSPRSSDWFFKTPLPTIIGSPTKTTAPFKIPKTQASSNLLALVTRHGPAE SQSKDGNDPRQCSHCLLVQGSERHRSEQQSKAVFGRSVSTGKLSEQQHVKAPLGGHQGSTDSLNTERPMD VAPAGACGVVLALPAGTAASARAVLFTVGSPPHSATAPTCTHMVLRTRTTSVGSNSSGGSLCSASGRVCV GSPPAPGFGSSPPGAEAAPSLRYVPYGASPPSLEGLITFEAPELPEETLMEREHTDTLRHLNMMLMFTEC VLDLTAVRGGNPELCTSAVSLYQIQESVVVDQISQLSKDWGRVEQLVLYMKAAQLLAASLHLAKAQIKSG KLSPSTAVKQVVKNLNERYKFCITMCKKLTEKLNRFFSDKQRFIDEINSVTAEKLIYNCAVEMVQSAALD EMFQQTEDIVQRYHKAALLLEGLSKILQDPADAESVHKYKCSIERRLSALCCSTAAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191645
ORF Size	3111 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191645.1, NP_001178574.1
RefSeq Size	6362 bp
RefSeq ORF	3114 bp
Locus ID	303206
MW	112.7 kDa

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