Nlrp3 (NM_001191642) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cias1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217429 representing NM_001191642 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGATGAGTGTTCGCTGCAAGCTGGCCCAGTATCTAGAGGACCTGGAAGATGTGGACCTCAAGA AATTTAAAATGCATTTGGAAGATTACCCACCCGAGAAAGGCTGTGTTCCGATCCCCAGGGGCCAGATGGA GAAGGCAGATCACTTGGATCTAGCCACACTCATGATTGACTTCAATGGGGAGGAGAAGGCGTGGGGCATG GCTGTGTGGATCTTTGCAGCGATCAACAGGCGAGACCTCTGGGAAAAAGCTAAGAAGGACCAGCCAGAGT GGAATGATGCATGCACGTCTAATCTCTCCATGGTGTGCCAGGAAGACAGCCTTGAAGAGGAGTGGATAGG TTTGCTGGGATATCTCTCCCGCATCTCGATTTGTAAAAAAAAGAAAGATTACTGTAAGATATACAGAAGG CATGTGAGAAGCAGGTTCTACTCCATCAAAGACAGGAATGCACGTCTAGGTGAGAGCGTGGACCTCAACA GACGCTACACCCAGCTCCAACTGGTCAAGGAGCATCCAAGCAAGCAGGAAAGGGAGCATGAACTCTTGAC CATTGGCAGGACTAAGATGTGGGACAGGCCCATGAGCTCCCTTAAGCTGGAGCTGCTCTTTGAGCCTGAG GATGAACACTTGGAGCCCGTGCACACAGTGGTGTTCCAGGGAGCAGCAGGCATCGGAAAAACAATCCTAG CCAGGAAGATTATGTTGGACTGGGCCTTGGGGAAGCTCTTCAAAGACAAATTTGATTATTTGTTCTTTAT CCACTGTCGAGAGGTGAGCCTCAGGGCACCAAAGAGCCTAGCAGATCTCATTATCAGCTGCTGGCCTGAC CCAAACCCACCAGTCTGCAAGATCCTGTGCAAGCCTTCCAGGATCCTCTTCCTCATGGACGGCTTTGATG AGCTGCAGGGGGCCTTTGATGAGCACATCGAGGAGGTCTGCACAGACTGGCAGAAGGCTGTGAGGGGAGA CATTCTGCTAAGCAGCCTCATCCGAAAGAAGTTGCTGCCTAAGGCCTCTCTGCTTATTACGACGAGGCCA GTAGCCTTGGAGAAGCTGCAGCATCTCCTGGACCACCCCCGCCACGTGGAGATCCTAGGTTTCTCTGAGG CCAAACGGAAGGAGTATTTCTTTAAGTATTTCTCAAACGAGCTGCAGGCCAGGGAGGCCTTCAGGCTGAT CCAAGAGAATGAGATCCTGTTTACTATGTGCTTCATCCCCCTGGTCTGCTGGATTGTGTGCACAGGGCTA AAGCAGCAGATGGAGACTGGAAAGAGCTTGGCCCAGACCTCCAAGACCACGACTGCCGTCTATGTCTTTT TCCTTTCCAGCCTGCTGCAGTCCCGGGGGGGCATTGAGGAGCACCTCTTCTCTGCCTACCTGCCAGGGCT CTGTTCATTGGCTGCGGATGGAATTTGGAACCAGAAGATCCTATTTGAAGAGTGTGATCTGCGGAAACAC GGCCTGCAGAAGACCGACGTCTCTGCTTTCCTGAGAATGAACGTGTTCCAGAAGGAGGTGGACTGCGAGA GATTTTACAGCTTCAGCCACATGACTTTCCAGGAGTTCTTTGCGGCTATGTACTATCTGCTAGAAGAGGA GGAAGAGGGGGTGACCGTGAGGAAGGGACCAGAAGGCTGTTCAGACCTTCTGAACCGAGACGTGAAGGTC CTATTAGAAAATTACGGCAAGTTCGAAAAAGGCTATCTGATTTTTGTTGTACGCTTCCTCTTTGGCCTTG TAAACCAGGAGAGAACTTCTTATTTGGAGAAGAAGCTAAGTTGCAAGATCTCTCAGCAAGTCAGACTGGA GCTGCTGAAGTGGATCGAAGTGAAAGCCAAGGCCAAGAAGCTGCAGAGGCAGCCCAGCCAGCTGGAACTG TTCTACTGCTTGTACGAGATGCAGGAGGAAGACTTTGTGCAGAGCGCCATGGGCCACTTCCCCAAAATTG AGATCAACCTCTCTACCAGAATGGACCACGTGGTTTCTTCCTTTTGCATTAAGAATTGCCATAGGGTAAA AACACTTTCCCTGGGATTTCTCCACAACTCACCCAAGGAGGAAGAAGAAGAGAAAAGAGGAAGTCAACCC TTGGACCAGGTCCAGTGTGTTTTCCCAGACCCTCATGTTGCCTGTTCTTCCAGACTGGTGAACTGCTGCC TCACCTCTAGCTTCTGCCGAGGTCTCTTCTCAAGTCTCAGCACCAACCAGAGCCTCACTGAACTGGACCT CAGTGACAATACTCTAGGGGATCCAGGCATGAGGGTGCTGTGTGAGGCACTCCAGCACCCAGGCTGTAAC ATTCAGAGACTGTGGTTGGGGCGCTGCGGACTGACCCATCAATGCTGTTTCAACATCTCCTCTGTCCTGA GCAGCAGCCAGAAGCTGGTGGAGCTGGACCTCAGTGACAATGCCCTGGGGGACTTTGGAGTCAGACTGCT GTGCGTGGGACTGAAGCATCTGCTCTGCAACCTGCAGAAACTCTGGTTGGTCAGCTGCTGTCTCACATCT GCGTGTTGTCAGGATCTCGCATTGGTTCTGAGCTCCAACCATTCTCTGACCAGACTGTACATTGGAGAAA ATGCCTTGGGAGACTCAGGAGTTCAAGTTTTGTGTGAAAAAATGAAGGACCCACAGTGTAACTTGCAGAA GCTGGGGTTGGTGAATTCCGGCCTTACTTCACTGTGCTGCTCAGCTCTGACCTCTGTGCTCAAAACCAAC CAGAACCTCACACACCTGTACCTACGAAGCAATGCCCTTGGAGACATGGGACTCAAGCTCCTCTGTGAGG GACTTCTGCACCCCGACTGTAAGCTACAGATGCTGGAGTTAGACAACTGCAGCCTCACCTCACACTCCTG CTGGGATCTCTCCACAATTCTGACCCATAACCAGAGCCTCCGGAAGCTGAACTTGAGCAACAACGACCTG GGCGATCTGTGTGTGGTGACGCTCTGTGAGGTTCTGAAACAGCAGGGCTGCCTCCTGCAGAGCCTACAGT TGGGTGAAATGTATTTAAATTGTGAAACAAAACGTACCTTAGAAGCTCTCCAGGAGGAAAAGCCTGAGCT GACTGTTGTTTTTGAGATTTCTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217429 representing NM_001191642 Red=Cloning site Green=Tags(s) MKMMSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCVPIPRGQMEKADHLDLATLMIDFNGEEKAWGM AVWIFAAINRRDLWEKAKKDQPEWNDACTSNLSMVCQEDSLEEEWIGLLGYLSRISICKKKKDYCKIYRR HVRSRFYSIKDRNARLGESVDLNRRYTQLQLVKEHPSKQEREHELLTIGRTKMWDRPMSSLKLELLFEPE DEHLEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRAPKSLADLIISCWPD PNPPVCKILCKPSRILFLMDGFDELQGAFDEHIEEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRP VALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEILFTMCFIPLVCWIVCTGL KQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGGIEEHLFSAYLPGLCSLAADGIWNQKILFEECDLRKH GLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEEEGVTVRKGPEGCSDLLNRDVKV LLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQRQPSQLEL FYCLYEMQEEDFVQSAMGHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFLHNSPKEEEEEKRGSQP LDQVQCVFPDPHVACSSRLVNCCLTSSFCRGLFSSLSTNQSLTELDLSDNTLGDPGMRVLCEALQHPGCN IQRLWLGRCGLTHQCCFNISSVLSSSQKLVELDLSDNALGDFGVRLLCVGLKHLLCNLQKLWLVSCCLTS ACCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSLCCSALTSVLKTN QNLTHLYLRSNALGDMGLKLLCEGLLHPDCKLQMLELDNCSLTSHSCWDLSTILTHNQSLRKLNLSNNDL GDLCVVTLCEVLKQQGCLLQSLQLGEMYLNCETKRTLEALQEEKPELTVVFEISW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191642
ORF Size	3105 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191642.1, NP_001178571.1
RefSeq Size	3857 bp
RefSeq ORF	3108 bp
Locus ID	287362
UniProt ID	D4A523
MW	118.5 kDa
Gene Summary	As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion (By similarity). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (By similarity). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (By similarity).[UniProtKB/Swiss-Prot Function]

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