Pkd2 (NM_001191934) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1559992; Trpp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217374 representing NM_001191934 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAACTCCAGCCGTGTGCAGCCGCAGCCTCCCGGGGACCCCGGACGCTCGCCCGCGCCGCGAGCGT CCGGGCCCGGCCGCCTGCTGGCGGGAGGCGCCGGCCTGGCAGTCCCCGGCGGCCTGCGGGAGCAGCGGGG CCTGGAGATTGAGATGGAGCGCATCCGGCAGGCGGCCGCTCGGGACCCACCGGCCGGAGCCTCGGCCTCG CCTTCTCCTCCGCTGTCGTCCTGCTCCAGGCAGGCATGGAGCCGCGACAACCCGGGCTTTGAGGCCGAAG AGGATGACGACGACGACGAGGTGGAAGGAGAAGAAGGAGGGATGGTGGTGGAGATGGACGTGGAGTGGCG TCCGGGCAGCCGAAGACCGGCCTCCTCTTCGGCCGTGAGCTCGGCGGGCGCCCGCGGCCGGGGTCTGGGA AGCTATCGCGGCGCGGCTTACCCGAGCGGGAGACGGCGCCGGCTAGAGGACCAGGGCGCGCCGTGTCCCA GCCCCGCGGGCGGCGGGGACCCGCTGCATCGCCACCTCCCGCTGGATGGCCAGCCGCCCCGAGTGGCCTG GGCCGAGAGGCTGGTGCGAGGACTGCGAGGTCTCTGGGGAACAAGACTCATGGAAGAGAGCAACGCTAAC CGAGAGAAATACCTGAGAAGCGTGTTGCGGGAGCTGGTCACTTACCTCTTTTTCCTCATACTCTTGTGCA TCTTGACCTATGGCATGATGAGCTCCAACGTGTACTACTACACTCGGACACTGTCACAACTTTTCATAGA CACCCCAGTGTCTAAAACAGAGAAAACCAACTTTAAAACTCTCTCTTCCATGGAGGACTTCTGGAAGTTC ACAGAAGGCTCCTTCCTGGATGGGCTGTACTGGAAGGCACAGAGCAGCAACCATACGCAAGCTGACAACC GGAGCTTTATTTTCTATGAGAACCTGCTGCTAGGAGTGCCGCGTCTACGCCAACTCCGAGTCAGAAACGG ATCCTGCTCCATCCCTCAGGACCTGAGAGATGAAATTAAAGAATGCTATGATGTCTACTCTGTCAGCAGT GAGGACAGGGCTCCATTTGGACCGCGGAACGGAACTGCGTGGATCTACACAAGTGAAAAAGACCTGAATG GCAGCAGTCACTGGGGAATCATTGCGACTTACAGTGGAGCCGGTTACTACCTGGATCTGTCCAGAACCAG GGAGGAGACAGCGGCCCAGCTTGCTGGCCTCAGGAGGAATTTCTGGCTGGACCGGGGCACACGGGCAGCT TTCATTGACTTCTCGGTGTATAACGCAAACATTAACCTGTTCTGTGTGGTCAGGTTATTGGTTGAGTTCC CAGCAACGGGTGGCGTGGTGCCCTCTTGGCAGTTTCAGCCTGTAAAACTGATCCGCTACGTCACAGCCTT TGATTTCTTCCTGGCAGCCTGTGAGATTATCTTTTGTTTCTTTATCCTTTACTATGTGGTGGAAGAGATA TTGGAAATTCGGATTCACAGACTGAACTATTTCAGGAGTTTCTGGAATTGTCTGGATGTTGTGATTGTTG TGTTGTCCGTAGTAGCCATGATGATTAACATTTACCGGATGTCCAATGCTGAGGGGCTGCTACAGTTTCT CGAAGATCAAAATTCTTTCCCCAACTTTGAGCATGTGGCATACTGGCAAATACAGTTCAACAATATAGCT GCCGTCATGGTATTTTTGGTCTGGATTAAGCTCTTCAAATTCATCAATTTCAATAGGACCATGAGTCAGC TCTCCACGACCATGTCCAGATGTGCCAAAGACCTCTTTGGCTTCACCATCATGTTCTTCATCATCTTCTT GGCATATGCCCAGTTGGCGTACCTTGTCTTTGGCACTCAGGTGGATGACTTCAGTACTTTCCAAGAGTGT ATTTTCACTCAGTTCCGCATCATTTTGGGTGATATCAACTTCGCAGAGATCGAGGAAGCTAACCGAGTTT TGGGGCCACTTTATTTTACTACATTTGTGTTCTTTATGTTCTTCATTCTTTTGAACATGTTTCTGGCGAT CATCAATGATTCCTACTCTGAGGTGAAATCAGATCTGGCCCAGCAGAAAGCAGAAATGGAACTCTCAGAC CTTATCAGAAAGGGCTGCCAAAAAGCACTTGTCAAACTAAAACTTAAAAGAAACACCGTAGACGCCATTT CAGAGAGTCTCCGGCAAGGTGGTGGCAAGCTAAACTTTGATGAACTTCGGCAAGACCTGAAAGGGAAGGG CCACACGGACGCAGAGATCGAGGCCATATTCACTAAATATGACCAGGACGGCGACCAGGAACTGACTGAG CGTGAGCATCAGCAGATGAGAGATGACTTGGAGAGAGAGAGGGAGGACCTAGACTTGGAACACAGCTCTT TACCACGTCCCATGAGCAGCAGAAGTTTCCCCAGAAGCCTGGATGACTCTGAGGAGGAAGACGATGAAGA CAGTGGCCATAGTTCCAGGAGGAGGGGAAGCATCTCCAGTGGGGTTTCCTATGAAGAGTTCCAAGTACTG GTGAGACGCGTGGACCGCATGGAGCACTCCATCGGCAGCATCGTGTCCAAGATCGATGCTGTGATTGTCA AGCTTGAGATCATGGAGCGGGCCAAGGTGAAGAGGCGAGAGGTGTTAGGAAGGCTGCTGGATGGAGTGGC TGAGGATGCGCGCCTGGGTCGGGATAGTGAGATCCACAGGGAGCAGATGGAGCGCCTGGTGCGGGAAGAG CTGGAGCGCTGGGAATCGGATGACGCAGCTTCACAGTCAGGTCACGGTCTAGGCACACAAGTGGGACTCG GTGGCCAGTCGCACCCCAGAAGCTCCCGGCCCCCTTCCTCCCAGTCTGCAGAGGGCCTGGAAGGCGGAGG TGGAAACGGAAGTACCAACGCGCATGCG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217374 representing NM_001191934 Red=Cloning site Green=Tags(s) MVNSSRVQPQPPGDPGRSPAPRASGPGRLLAGGAGLAVPGGLREQRGLEIEMERIRQAAARDPPAGASAS PSPPLSSCSRQAWSRDNPGFEAEEDDDDDEVEGEEGGMVVEMDVEWRPGSRRPASSSAVSSAGARGRGLG SYRGAAYPSGRRRRLEDQGAPCPSPAGGGDPLHRHLPLDGQPPRVAWAERLVRGLRGLWGTRLMEESNAN REKYLRSVLRELVTYLFFLILLCILTYGMMSSNVYYYTRTLSQLFIDTPVSKTEKTNFKTLSSMEDFWKF TEGSFLDGLYWKAQSSNHTQADNRSFIFYENLLLGVPRLRQLRVRNGSCSIPQDLRDEIKECYDVYSVSS EDRAPFGPRNGTAWIYTSEKDLNGSSHWGIIATYSGAGYYLDLSRTREETAAQLAGLRRNFWLDRGTRAA FIDFSVYNANINLFCVVRLLVEFPATGGVVPSWQFQPVKLIRYVTAFDFFLAACEIIFCFFILYYVVEEI LEIRIHRLNYFRSFWNCLDVVIVVLSVVAMMINIYRMSNAEGLLQFLEDQNSFPNFEHVAYWQIQFNNIA AVMVFLVWIKLFKFINFNRTMSQLSTTMSRCAKDLFGFTIMFFIIFLAYAQLAYLVFGTQVDDFSTFQEC IFTQFRIILGDINFAEIEEANRVLGPLYFTTFVFFMFFILLNMFLAIINDSYSEVKSDLAQQKAEMELSD LIRKGCQKALVKLKLKRNTVDAISESLRQGGGKLNFDELRQDLKGKGHTDAEIEAIFTKYDQDGDQELTE REHQQMRDDLEREREDLDLEHSSLPRPMSSRSFPRSLDDSEEEDDEDSGHSSRRRGSISSGVSYEEFQVL VRRVDRMEHSIGSIVSKIDAVIVKLEIMERAKVKRREVLGRLLDGVAEDARLGRDSEIHREQMERLVREE LERWESDDAASQSGHGLGTQVGLGGQSHPRSSRPPSSQSAEGLEGGGGNGSTNAHA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191934
ORF Size	2898 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191934.1, NP_001178863.1
RefSeq Size	4851 bp
RefSeq ORF	2901 bp
Locus ID	498328
MW	109.1 kDa

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