Prkd1 (NM_001276715) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	nPKC-D1; nPKC-mu; Pkcm; Prkcm
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217323 representing NM_001276715 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCCCCTCCGCTACTACGGCCGCCTAGCCCGCTGCTGCCCGCCGCGGCCGCTGTGGCCGCCGCCG CTGCTGCGCTGGTCCCGGGCTCCGGGCCCGCGCCCTTCCCAGCGCCTGGGGCCGCCCCGGCGGGGGGCAT CTCGTTCCATCTGCAGATCGGCCTGAGCCGCGAGCCGGTGCTGCTCCTTCAGGACTCCTCTGGCGACTAC AGCCTGGCGCATGTCCGGGAGATGGCTTGCTCCATCGTGGACCAGAAGTTCCCTGAATGTGGCTTCTATG GACTCTATGACAAGATCCTGCTTTTCCGTCACGATCCTGCCTCTGAAAACATCCTTCAGCTGGTAAAAAT CGCAAGTGACATTCAGGAGGGCGATCTTATTGAAGTGGTCCTGTCAGCTTCAGCCACCTTCGAAGACTTC CAGATCCGGCCTCACGCTCTCTTCGTTCATTCATACAGAGCCCCTGCTTTCTGTGATCACTGTGGAGAAA TGCTGTGGGGACTGGTGCGCCAAGGCCTTAAATGTGAAGGATGTGGCCTGAATTACCATAAGAGATGTGC ATTTAAAATCCCCAACAATTGCAGTGGAGTGAGAAGGAGAAGGCTCTCAAATGTTTCCCTCACTGGACTG GGTACTGTCCGCACAGCGTCTGCGGAGTTCTCCACCAGTGCCCCCGATGAGCCTCTACTGTCTCCTGTGA GCCCTGGCTTTGAGCAAAAGTCTCCATCTGAGTCATTTATCGGTCGTGAGAAGAGGTCAAATTCTCAGTC ATATGTTGGACGGCCGATTCAGCTCGACAAGCTCCTGATGTCTAAGGTGAAGGTGCCACACACCTTTGTC ATCCACTCCTACACACGGCCCACGGTCTGCCAGTTCTGTAAGAAGCTCCTCAAGGGCCTCTTCCGGCAGG GTTTGCAGTGCAAAGATTGCCGATTCAACTGTCACAAACGCTGTGCACCAAAAGTACCAAACAACTGCTT GGGCGAAGTGACCATCAATGGAGAATTGCTTAGCCCTGGGGCAGAGTCTGATGTTGTCATGGAAGAAGGG AGCGATGACAATGACAGCGAACGGAACAGTGGACTCATGGATGACATGGACGAGGCCATGGTCCAGGACA CTGAGATGGCTTTGGCGGAGGGTCAGAGTGACGGTGCAGAGATGCAGGATCCAGACGCAGACCAGGAGGA CTCCAACAGAACCATCAGCCCTTCTACGAGCAACAACATACCGCTTATGAGGGTAGTGCAGTCTGTCAAG CACACAAAGAGGAGAAGCAGCACAGTGATGAAGGAAGGGTGGATGGTCCACTACACCAGCAAGGACACAC TGAGGAAAAGGCATTACTGGAGACTGGACAGCAAAAGCATCACACTCTTCCAAAACGACACAGGCAGCCG GTACTACAAGGAAATTCCTTTATCAGAAATTTTATGTCTGGAACCAGCAAAACCTTCAGCATTAATTCCC ACTGGAGCTAACCCTCATTGTTTTGAAATCACTACAGCAAACGTAGTGTATTATGTGGGAGAAAACGTGG TCAACCCTTCAAGCCCCCCACCAAACAACAGCGTCCCCCCGAGCGGCATCGGTACCGATGTGGCGAGGAT GTGGGAGGTCGCCATCCAGCACGCTCTCATGCCTGTCATCCCCAAGGGCTCCTCTGTGGGTTCCGGAACC AACTCACACAAAGATATTTCGGTGAGCATTTCCGTTTCGAATAGCCAGATTCAGGAAAATGTGGATATCA GCACAGTCTATCAGATTTTCCCGGATGAGGTTCTGGGTTCTGGACAGTTCGGAATTGTTTATGGAGGTAA ACATCGTAAAACAGGAAGAGATGTAGCTATTAAGATTATTGACAAATTAAGATTTCCAACAAAACAAGAA AGTCAGCTTCGTAATGAGGTTGCAATTTTACAGAACCTTCATCACCCTGGTGTTGTAAATTTGGAGTGTA TGTTTGAGACGCCTGAAAGAGTGTTTGTTGTTATGGAAAAACTCCATGGAGACATGCTGGAGATGATCCT GTCAAGTGAAAAGGGCAGGTTGCCAGAACACATAACGAAGTTTTTAATTACTCAGATACTAGTGGCTTTG CGACATCTTCATTTTAAAAACATCGTTCACTGTGACCTCAAGCCAGAAAATGTGTTGCTGGCATCAGCAG ACCCTTTCCCTCAGGTGAAACTTTGTGATTTTGGTTTTGCCCGGATCATTGGCGAGAAGTCTTTCCGGAG ATCAGTGGTGGGTACCCCAGCCTACCTGGCACCTGAGGTTCTGAGGAACAAGGGCTATAACCGCTCGCTA GACATGTGGTCTGTTGGGGTCATCATCTATGTGAGCCTGAGTGGCACCTTCCCTTTTAATGAAGATGAAG ATATCCACGATCAGATCCAGAACGCAGCCTTCATGTATCCACCCAATCCCTGGAAGGAGATTTCTCATGA AGCCATTGATCTTATCAATAACTTGCTACAAGTGAAAATGAGAAAACGCTACAGTGTGGACAAGACCTTG AGTCACCCCTGGCTACAGGACTACCAGACCTGGTTAGATTTACGCGAGCTGGAATGCAGAATTGGAGAAC GCTATATTACCCATGAAAGCGATGACTCGAGGTGGGAACAGTATGCAGGCGAGCAGGGACTGCAGTATCC CGCGCACCTGATCAATCTGAGTGCTAGCCATGGCGACAGTCCTGAGGCTGAAGAGAGAGAGATGAAAGCC CTCAGTGAGCGTGTCAGCATCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217323 representing NM_001276715 Red=Cloning site Green=Tags(s) MSAPPLLRPPSPLLPAAAAVAAAAAALVPGSGPAPFPAPGAAPAGGISFHLQIGLSREPVLLLQDSSGDY SLAHVREMACSIVDQKFPECGFYGLYDKILLFRHDPASENILQLVKIASDIQEGDLIEVVLSASATFEDF QIRPHALFVHSYRAPAFCDHCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRRRRLSNVSLTGL GTVRTASAEFSTSAPDEPLLSPVSPGFEQKSPSESFIGREKRSNSQSYVGRPIQLDKLLMSKVKVPHTFV IHSYTRPTVCQFCKKLLKGLFRQGLQCKDCRFNCHKRCAPKVPNNCLGEVTINGELLSPGAESDVVMEEG SDDNDSERNSGLMDDMDEAMVQDTEMALAEGQSDGAEMQDPDADQEDSNRTISPSTSNNIPLMRVVQSVK HTKRRSSTVMKEGWMVHYTSKDTLRKRHYWRLDSKSITLFQNDTGSRYYKEIPLSEILCLEPAKPSALIP TGANPHCFEITTANVVYYVGENVVNPSSPPPNNSVPPSGIGTDVARMWEVAIQHALMPVIPKGSSVGSGT NSHKDISVSISVSNSQIQENVDISTVYQIFPDEVLGSGQFGIVYGGKHRKTGRDVAIKIIDKLRFPTKQE SQLRNEVAILQNLHHPGVVNLECMFETPERVFVVMEKLHGDMLEMILSSEKGRLPEHITKFLITQILVAL RHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSL DMWSVGVIIYVSLSGTFPFNEDEDIHDQIQNAAFMYPPNPWKEISHEAIDLINNLLQVKMRKRYSVDKTL SHPWLQDYQTWLDLRELECRIGERYITHESDDSRWEQYAGEQGLQYPAHLINLSASHGDSPEAEEREMKA LSERVSIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276715
ORF Size	2754 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276715.1, NP_001263644.1
RefSeq Size	2757 bp
RefSeq ORF	2757 bp
Locus ID	85421
UniProt ID	Q9WTQ1
MW	102 kDa
Gene Summary	serine/threonine kinase; may play a role in the regulation of physiological events such as ion and protein secretion; may mediate cell proliferation or differentiation through participation in various signalling cascades [RGD, Feb 2006]

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