Ntrk3 (NM_001270656) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	trkC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217278 representing NM_001270656 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTCTCTCTTTGCCCAGCCAAGTGTAGTTTCTGGCGGATTTTCTTGCTGGGAAGCGTCTGGCTGG ACTATGTGGGCTCCGTGCTGGCTTGCCCTGCAAATTGTGTCTGCAGCAAGACTGAGATCAATTGCCGGCG GCCGGACGATGGGAACCTCTTTCCCCTCCTGGAAGGGCAGGATTCAGGGAACAGCAATGGGAACGCCAGC ATCAACATCACGGACATCTCAAGGAATATCACTTCCATACACATAGAGAACTGGCGGGGCCTGCACACAC TCAATGCTGTGGATATGGAGCTCTACACGGGACTCCAGAAGCTGACCATCAAGAATTCTGGACTCCGGAA TATTCAGCCCAGAGCCTTTGCTAAGAATCCCCACTTGCGTTATATAAACTTGTCAAGTAACCGGCTCACC ACACTCTCCTGGCAGCTCTTCCAGACGCTGAGTCTTCGGGAATTGAGACTGGAGCAGAACTTCTTCAACT GCAGCTGTGACATCCGCTGGATGCAGCTGTGGCAGGAACAGGGGGAGGCACGGCTGGACAGCCAGAGCCT CTACTGCATCAGTGCTGATGGCTCCCAACTCCCCCTCTTCCGCATGAACATCAGTCAGTGTGATCTCCCT GAGATCAGTGTGAGTCACGTGAACCTGACTGTCCGAGAAGGAGACAATGCTGTAATCACTTGTAATGGCT CTGGCTCTCCTTTGCCTGATGTGGACTGGATAGTCACCGGACTGCAGTCCATCAATACTCATCAGACCAA TCTGAACTGGACCAATGTACATGCCATCAACTTGACCCTGGTGAATGTGACGAGCGAAGACAATGGTTTC ACCCTGACGTGCATTGCAGAGAACGTGGTGGGCATGAGCAATGCCAGCGTTGCTCTCACTGTCTACTACC CTCCACGTGTGGTGAGCTTGGTGGAGCCTGAGGTACGCCTGGAACATTGCATTGAGTTTGTGGTGCGTGG CAACCCGACACCCACGCTGCATTGGCTGTACAATGGACAGCCACTGAGGGAATCCAAGATCATTCATATG GACTACTACCAGGAGGGTGAAGTCTCAGAGGGCTGCCTGCTCTTCAACAAGCCCACCCACTACAACAATG GCAACTACACCCTCATTGCTAAGAATGCCCTGGGCACAGCCAACCAGACCATCAACGGCCACTTCCTTAA GGAGCCCTTTCCAGAGAGCACAGATTTCTTTGACTTTGAGTCTGATGCGAGCCCTACACCTCCTATCACT GTGACCCACAAACCAGAGGAAGACACTTTTGGGGTGTCCATAGCAGTTGGACTTGCTGCTTTTGCCTGCG TCCTTCTGGTGGTCCTCTTTATCATGATCAACAAGTACGGTCGACGGTCCAAATTTGGAATGAAGGGTCC TGTGGCTGTTATCAGTGGGGAGGAGGACTCAGCCAGCCCACTGCACCATATCAACCATGGCATCACTACA CCTTCATCGCTGGATGCTGGGCCGGATACAGTGGTCATTGGCATGACTCGAATTCCAGTCATTGAGAACC CCCAATATTTCCGTCAGGGACACAATTGCCACAAGCCAGACACATATGTTCAACACATCAAGAGGAGAGA CATCGTGTTGAAGAGAGAACTGGGTGAGGGAGCCTTTGGAAAGGTCTTCCTGGCTGAGTGCTACAATCTA AGCCCCACCAAAGACAAGATGCTTGTGGCAGTGAAGGCCCTGAAGGATCCCACCTTGGCTGCCAGGAAGG ATTTCCAGAGGGAGGCCGAGCTGCTCACTAACCTGCAGCATGAGCATATTGTCAAATTCTATGGGGTCTG TGGTGATGGGGATCCACTCATCATGGTCTTTGAATACATGAAGCATGGAGACCTGAACAAGTTCCTCAGG GCCCATGGGCCAGATGCGATGATCCTTGTGGATGGACAGCCACGCCAGGCCAAGGGGGAGCTAGGACTCT CCCAGATGCTCCACATCGCCAGTCAGATAGCCTCCGGCATGGTGTACTTGGCCTCCCAGCACTTTGTGCA CCGGGACCTGGCCACCAGGAACTGCCTAGTTGGAGCCAACCTTCTAGTGAAGATTGGAGATTTTGGCATG TCCAGGGACGTCTACAGTACTGATTACTACAGGGAAGGGCCATACCAGAAGGGCCCATTCAGCGTGTCGT GGCAGCAACAGAGGCTAGCAGCATCAGCAGCTTCCACACTCTTTAATCCATCTGGAAATGATTTTTGTAT ATGGTGTGAGGTGGGAGGACACACCATGCTCCCCATCCGCTGGATGCCACCTGAAAGCATCATGTACCGG AAGTTTACCACAGAGAGTGATGTGTGGAGCTTTGGGGTTATTCTTTGGGAGATCTTTACCTATGGGAAGC AACCATGGTTCCAGCTCTCTAACACAGAGGTCATTGAATGCATCACCCAAGGCCGTGTCTTGGAGAGACC CCGAGTCTGCCCAAAAGAAGTATATGATGTCATGCTGGGGTGCTGGCAGAGGGAACCACAACAGCGACTG AATATTAAGGAGATCTACAAAATCCTCCATGCTTTGGGGAAGGCCACCCCAATCTACCTGGACATTCTTG GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217278 representing NM_001270656 Red=Cloning site Green=Tags(s) MDVSLCPAKCSFWRIFLLGSVWLDYVGSVLACPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNAS INITDISRNITSIHIENWRGLHTLNAVDMELYTGLQKLTIKNSGLRNIQPRAFAKNPHLRYINLSSNRLT TLSWQLFQTLSLRELRLEQNFFNCSCDIRWMQLWQEQGEARLDSQSLYCISADGSQLPLFRMNISQCDLP EISVSHVNLTVREGDNAVITCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNGF TLTCIAENVVGMSNASVALTVYYPPRVVSLVEPEVRLEHCIEFVVRGNPTPTLHWLYNGQPLRESKIIHM DYYQEGEVSEGCLLFNKPTHYNNGNYTLIAKNALGTANQTINGHFLKEPFPESTDFFDFESDASPTPPIT VTHKPEEDTFGVSIAVGLAAFACVLLVVLFIMINKYGRRSKFGMKGPVAVISGEEDSASPLHHINHGITT PSSLDAGPDTVVIGMTRIPVIENPQYFRQGHNCHKPDTYVQHIKRRDIVLKRELGEGAFGKVFLAECYNL SPTKDKMLVAVKALKDPTLAARKDFQREAELLTNLQHEHIVKFYGVCGDGDPLIMVFEYMKHGDLNKFLR AHGPDAMILVDGQPRQAKGELGLSQMLHIASQIASGMVYLASQHFVHRDLATRNCLVGANLLVKIGDFGM SRDVYSTDYYREGPYQKGPFSVSWQQQRLAASAASTLFNPSGNDFCIWCEVGGHTMLPIRWMPPESIMYR KFTTESDVWSFGVILWEIFTYGKQPWFQLSNTEVIECITQGRVLERPRVCPKEVYDVMLGCWQREPQQRL NIKEIYKILHALGKATPIYLDILG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270656
ORF Size	2592 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270656.1, NP_001257585.1
RefSeq Size	2824 bp
RefSeq ORF	2595 bp
Locus ID	29613
UniProt ID	Q03351
MW	97.1 kDa
Gene Summary	a tyrosine-protein kinase receptor for neurotrophin-3 [RGD, Feb 2006]

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