Cdh3 (NM_053938) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217235 representing NM_053938 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGCTTAGTGGGCCCCTGGTCTTCCTGCTCCTCCTGCTCCAGGTTTGCTGGCTACGCAGCGTGG TCTCCGAGTCCTACCGGGAGGACTTCATCCGGGAGGCTGGAGTGACCTTGGAGGTGGGAGGAACTGACCT GGAGCCGAGCCAAGCGCTAGAGAAAGAGGCATTGGCTGGACACGAGGTGCTCGGTGCAGACAGTGGAGGA ATCATTACACTGAATAGGGAGACAGTCCAGGGAGGGAAGGGTGTGATGAACTCCCCACCCTCACGCATCT TACGGAGACGAAAGAGAGAGTGGGTGATGCCACCAATATCGGTCCCTGAGAATGGCAAAGGTCCTTTTCC TCAGAGGCTGAATCAGCTTAAATCTAATAAGGACAGAGGCACCAAGCTTTTCTACAGCATCACGGGGCCT GGAGCTGACAGTCCCCCTGAGGGAGTCTTCACCATAGAGAAGGAGACGGGCTGGCTGCTGTTGAATATGC CACTGGACAGGGAGAAAATTGTCAAGTATGAGCTTTTTGGCCACGCTGTATCTGAGAATGGTGCGTCTGT AGAGGAGCCCATGAACATATCCATCATTGTGACAGATCAGAATGACAACAAGCCCAAGTTCACTCAAGAC ACCTTCAGAGGGAGTGTTCCGGAGGGGGTAATGCCTGGCACTTCTGTGATGCAGGTGACAGCCACAGATG AGGACGACGCCATCAATACTTACAATGGGGTGGTGGCTTACTCCATCCATAGCCAAGAGCCGAAGGAGCC ACACGACCTCATGTTCACCATCCATAAAAGCACTGGGACTATTAGCGTCATCTCCAGTGGCCTGGATAGA GAGAAAGTCCCTGAGTACACACTGACCATCCAGGCCACAGACATGGACGGAGACGGCTCTACCACAACAG CCGAGGCCGTAGTGCAGATCCTTGATACCAATGATAACGCTCCAGAGTTCCAGCCGCAAAAGTATGAGGC TTGGGTGCCTGAGAATGCAGTGGGCCACGAGGTACAGAGGCTGACAGTGACCGATCTGGATTCCCCCAAC TCACCGGCATGGCGTGCCACCTACCACATTGTGGGAGGTGACGATGGGGATCATTTCACCGTGGCCACTC ACCCAGAGACCAACCAAGGCATCCTGACAACCAAGAAGGGTTTGGATTTTGAGGCTCAGAACCAACACAC CCTGTACATAGAAGTGACCAACGAGGCTTCCTTTGCAGTGAAGCTCCCGACTGCCACCGCCACCGTGGTG GTCCATGTGGAAGATGTCAACGAGGCCCCTGTGTTTGTTCCACCTTCCAAGGTCATTGAGGCCCAGGAAG GCATCTCTATTGGGGAACTGGTCTGTGTCTATACTGCACAGGACCCTGACAAGGAGGAACAGAAGATCAG CTACAGCATCTTGAGAGACCCAGCCAGCTGGCTTGCTGTGGACCCGGACAGTGGTCAGATAACGGCTGCA GGCATCTTAGATCGTGAGGACGAGCAGTTTGTGAAAAACGACATCTACGAAGTCATGGTGTTGGCCACAG ACAATGGAAACCCTCCCACCACCGGCACTGGGACCCTCCTGCTAACACTTACTGACATCAATGACCATGG CCCAATCCCTGAACCTCGTCAGATCATCATCTGTAACCAAAGCCCCGTGCCTCAAGTGCTGAACATCACA GACAAGGACCTGTCCCCCAACTCCTCCCCTTTCCAGGCCCAGCTCACACATGATTCAGATATCTACTGGA TGGCAGAAGTCAGCGAGAAAGGAGACACCGTGGCCTTGTCCCTGAAGAAGTTCCTGAAGCAAGACACATA TGACTTGCACCTTTCTTTGTCTGACCATGGCAACCGGGAACAGCTAACCATGATCAGGGCCACTGTGTGT GACTGCCACGGCCAGGTGTTGAATGACTGCCCCAGACCCTGGAAAGGTGGCTTCATCCTCCCCGTCCTGG GTGCTGTCCTGGCTCTACTGACCCTTCTTCTGGCGCTCCTTCTGTTGGTGAGAAAGAAGAGGAAGGTCAA AGAGCCCCTTCTGCTCCCAGAGGATGACACTCGTGACAATGTCTTCTATTATGGAGAAGAGGGTGGTGGC GAAGAGGACCAGGACTATGACATCACTCAACTCCACCGGGGACTAGAGGCCAGGCCTGAGGTGGTTCTCC GCAACGACGTGGCACCAACCTTCATCCCCACCCCCATGTACCGCCCCCGGCCCGCCAACCCAGATGAAAT CGGCAACTTCATCATCGAGAACCTGAAAGCTGCCAACACAGACCCCACTGCTCCTCCCTACGACTCCCTG CTGGTGTTTGACTACGAGGGCAGTGGCTCTGATGCTGCCTCCCTGAGCTCCCTCACCTCCTCCACCTCTG ATCAGGACCAGGACTACAACTACCTTACCGAGTGGGGAAGTCGATTCAAGAAACTGGCTGACATGTATGG TGGCGGTGAGGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217235 representing NM_053938 Red=Cloning site Green=Tags(s) MELLSGPLVFLLLLLQVCWLRSVVSESYREDFIREAGVTLEVGGTDLEPSQALEKEALAGHEVLGADSGG IITLNRETVQGGKGVMNSPPSRILRRRKREWVMPPISVPENGKGPFPQRLNQLKSNKDRGTKLFYSITGP GADSPPEGVFTIEKETGWLLLNMPLDREKIVKYELFGHAVSENGASVEEPMNISIIVTDQNDNKPKFTQD TFRGSVPEGVMPGTSVMQVTATDEDDAINTYNGVVAYSIHSQEPKEPHDLMFTIHKSTGTISVISSGLDR EKVPEYTLTIQATDMDGDGSTTTAEAVVQILDTNDNAPEFQPQKYEAWVPENAVGHEVQRLTVTDLDSPN SPAWRATYHIVGGDDGDHFTVATHPETNQGILTTKKGLDFEAQNQHTLYIEVTNEASFAVKLPTATATVV VHVEDVNEAPVFVPPSKVIEAQEGISIGELVCVYTAQDPDKEEQKISYSILRDPASWLAVDPDSGQITAA GILDREDEQFVKNDIYEVMVLATDNGNPPTTGTGTLLLTLTDINDHGPIPEPRQIIICNQSPVPQVLNIT DKDLSPNSSPFQAQLTHDSDIYWMAEVSEKGDTVALSLKKFLKQDTYDLHLSLSDHGNREQLTMIRATVC DCHGQVLNDCPRPWKGGFILPVLGAVLALLTLLLALLLLVRKKRKVKEPLLLPEDDTRDNVFYYGEEGGG EEDQDYDITQLHRGLEARPEVVLRNDVAPTFIPTPMYRPRPANPDEIGNFIIENLKAANTDPTAPPYDSL LVFDYEGSGSDAASLSSLTSSTSDQDQDYNYLTEWGSRFKKLADMYGGGEDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053938
ORF Size	2466 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053938.2, NP_446390.2
RefSeq Size	3458 bp
RefSeq ORF	2469 bp
Locus ID	116777
MW	90.4 kDa
Gene Summary	cell-cell adhesion molecule [RGD, Feb 2006]

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