Ercc2 (NM_001172809) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217178 representing NM_001172809 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTCAACGTGGATGGGCTGCTGGTCTACTTCCCGTACGACTACATCTACCCGGAGCAGTTCTCCT ACATGCTGGAGCTCAAGCGCACGCTCGACGCCAAGGGTCATGGAGTCCTGGAGATGCCCTCAGGCACTGG GAAGACAGTGTCCCTGTTGGCCCTGATCGTGGCCTATCAACGGGCTTATCCACTGGAGGTGACCAAACTC ATCTACTGCTCACGGACCGTGCCGGAGATTGAGAAGGTCATAGAAGAGCTTCGTAAGCTGCTCAGTTTCT ACGAGCAGCAGGAGGGTGAGAAGCTGCCATTCTTAGGACTGGCTCTGAGCTCGAGAAAGAACCTGTGCAT TCATCCCGAGGTGACACCACTGCGCTTCGGGAAGGACGTTGATGGGAAGTGTCACAGCCTGACAGCGTCA TACGTGCGGGCACAGTACCAGCAGGATGCCAGCCTACCTCACTGCCGTTTCTATGAGGAATTTGACACCC ATGGACGCCAGGTGCCCCTCCCTGCGGGCATCTACAACCTGGATGACTTGAAGGCCCTGGGGCAGCGGCA GGGCTGGTGCCCCTACTTCCTGGCTCGATACTCGATCCTGCATGCCAACGTGGTGGTTTACAGCTACCAC TACCTCCTGGACCCCAAGATCGCAGACCTGGTATCCAAAGAGCTGGCCCGCAAGGCCGTTGTGGTCTTCG ATGAAGCCCACAATATCGACAATGTCTGCATCGACTCCATGAGTGTCAACCTCACCCGCAGGACTCTGGA CCGTTGCCAAAGCAACTTAGACACCCTGCAGAAGACGGTGCTCAGGATCAAGGAGACAGATGAGCAGCGG CTACGAGATGAGTACCGGCGCCTGGTGGAGGGCCTGCGGGAGGCCAGTGCAGCTCGGGAGACAGATGCCC ACCTGGCCAACCCTGTGCTGCCCGATGAGGTGCTGCAGGAGGCCGTGCCAGGCTCCATCCGCACCGCCGA GCACTTCCTGGGCTTCCTGCGGCGGCTGCTGGAGTATGTCAAGTGGCGGCTGCGTGTGCAGCATGTGGTG CAGGAGAGTCCACCTGCCTTTCTGAGCGGCCTGGCCCAGCGTGTGTGCATCCAGCGCAAGCCCCTCAGGT TCTGTGCCGAGCGCCTGCGCTCCCTGCTGCACACCCTGGAGATTGCTGATCTGGCCGACTTCTCCCCACT CACACTCCTTGCCAACTTCGCCACTCTCGTCAGCACTTATGCCAAGGGCTTCACCATTATCATTGAACCC TTTGACGACAGGACCCCCACCATCACCAACCCCATTCTGCACTTCAGCTGTATGGACGCCTCCTTGGCCA TCAAGCCTGTGTTTGAACGCTTCCAGTCTGTCATCATCACGTCCGGGACACTGTCTCCACTGGACATCTA CCCCAAGATCCTGGACTTCCACCCGGTTACAATGGCAACCTTCACCATGACACTGGCCCGAGTCTGCCTC TGCCCAATGATCATTGGCCGTGGTAATGACCAGGTAGCAATCAGTTCCAAATTTGAGACCAGAGAAGATA TCGCTGTAATCCGCAACTATGGCAACCTCCTGCTGGAGATGTCTGCCGTGGTCCCTGATGGCATCGTGGC CTTCTTCACCAGCTACCAGTACATGGAAAGCACTGTAGCCTCCTGGTATGAGCAGGGCATCCTTGAAAAC ATCCAGAGAAACAAACTGCTCTTCATTGAGACCCAGGATGGGGCTGAGACCAGTGTGGCCCTGGAGAAGT ACCAAGAGGCATGTGAGAATGGCCGCGGGGCCATTCTGCTCTCAGTGGCTCGGGGCAAAGTATCAGAAGG GATTGACTTTGTGCACCACTATGGGCGGGCCGTGATCATGTTTGGCGTCCCCTATGTTTACACCCAGAGC CGAATTCTCAAGGCCAGGCTAGAGTATCTGCGGGACCAGTTCCAGATCCGAGAGAATGACTTCCTCACCT TTGATGCCATGCGCCATGCAGCGCAGTGTGTGGGTCGCGCTATCAGGGGCAAGACAGACTATGGACTCAT GGTCTTTGCTGACAAGCGGTTTGCTCGAGCTGACAAGCGTGGGAAGCTGCCTCGATGGATCCAGGAGCAT CTGACTGACTCCAACCTCAACCTGACCGTGGATGAGGGTGTACAGGTCGCCAAGTACTTCCTGCGGCAGA TGGCACAGCCCTTCCACCGGGAGGATCAGTTGGGTCTGTCACTGCTCAGTCTGGAGCAGCTGCAGTCCGA GGAAACACTACAGCGAATTGAGCAGATTGCACAGCAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217178 representing NM_001172809 Red=Cloning site Green=Tags(s) MKLNVDGLLVYFPYDYIYPEQFSYMLELKRTLDAKGHGVLEMPSGTGKTVSLLALIVAYQRAYPLEVTKL IYCSRTVPEIEKVIEELRKLLSFYEQQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTAS YVRAQYQQDASLPHCRFYEEFDTHGRQVPLPAGIYNLDDLKALGQRQGWCPYFLARYSILHANVVVYSYH YLLDPKIADLVSKELARKAVVVFDEAHNIDNVCIDSMSVNLTRRTLDRCQSNLDTLQKTVLRIKETDEQR LRDEYRRLVEGLREASAARETDAHLANPVLPDEVLQEAVPGSIRTAEHFLGFLRRLLEYVKWRLRVQHVV QESPPAFLSGLAQRVCIQRKPLRFCAERLRSLLHTLEIADLADFSPLTLLANFATLVSTYAKGFTIIIEP FDDRTPTITNPILHFSCMDASLAIKPVFERFQSVIITSGTLSPLDIYPKILDFHPVTMATFTMTLARVCL CPMIIGRGNDQVAISSKFETREDIAVIRNYGNLLLEMSAVVPDGIVAFFTSYQYMESTVASWYEQGILEN IQRNKLLFIETQDGAETSVALEKYQEACENGRGAILLSVARGKVSEGIDFVHHYGRAVIMFGVPYVYTQS RILKARLEYLRDQFQIRENDFLTFDAMRHAAQCVGRAIRGKTDYGLMVFADKRFARADKRGKLPRWIQEH LTDSNLNLTVDEGVQVAKYFLRQMAQPFHREDQLGLSLLSLEQLQSEETLQRIEQIAQQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001172809
ORF Size	2280 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001172809.1, NP_001166280.1
RefSeq Size	3548 bp
RefSeq ORF	2283 bp
Locus ID	308415
MW	86.8 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.