Rangap1 (NM_001244855) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216957 representing NM_001244855 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCTGAAGACATTGCCAAATTGGCAGAGACCCTGGCCAAAACTCAGGTAGCCGGAGGACAGCTGA GTTTTAAGGGCAAGGGTCTCAAACTAAATACTGCAGAAGATGCTAAAGATGTGATTAAGGAGATTGAAGA GTTTGATGGCCTGGAAGCTCTACGACTGGAGGGGAACACAGTGGGCGTGGAAGCGGCCAGAGTCATCGCC AAGGCCTTAGAGAAGAAGTCCAAGCTGAAGCGATGCCATTGGAGTGACATGTTCACCGGAAGGCTTCGGT CCGAGATCCCACCAGCTCTGATCTCACTAGGGGAGGGGCTCATCACTGCAGGAGCACAGCTGGTGGAGCT CGACCTCAGTGACAACGCATTTGGACCTGATGGAGTGCGAGGCTTCGAGGCCCTCCTCAAGAGCCCAGCC TGCTTCACCCTGCAGGAACTCAAGCTTAACAACTGTGGCATGGGCATCGGTGGTGGCAAGATTCTGGCAG CAGCTCTGACTGAGTGCCACCGCAAGTCCAGTGCCCAAGGCAAGCCACTGACCCTGAAGGTGTTTGTGGC TGGCAGAAATCGCCTGGAGAATGATGGAGCCACTGCACTGGCAGAAGCCTTTGGGATCATCGGGACTCTG GAAGAGGTCCATATGCCACAGAATGGAATTAACCACCCTGGTGTCACAGCCCTGGCCCAGGCGTTTGCCA TTAACCCCCTGCTGCGAGTCATCAATCTAAACGACAACACCTTCACTGAGAAGGGTGGGGTGGCCATGGC TGAGACCCTGAAGACACTGCGACAAGTGGAGGTGATCAATTTTGGAGACTGCCTGGTGCGCTCCAAGGGG GCGGTCGCCATCGCAGATGCTGTCCGTGGGGGCCTGCCCAAGCTGAAGGAGCTGAACCTGTCATTCTGTG AAATCAAGAGAGATGCTGCCCTGGTTGTTGCCGAGGCTGTGGCTGACAAGGCTGAGCTGGAGAAGCTGGA CCTCAATGGCAATGCCCTAGGAGAAGAGGGCTGTGAGCAGCTTCAGGAGGTGCTGGACAGCTTCAATATG GCCAAGGTGCTGGCATCCCTCAGTGATGATGAGGGAGAGGATGAGGATGAGGATGAGGAAGGAGAGGACG ATGAGGATGAGGAAGATGAGGAGGAGGAGGAGGAAGAAGAGGAGGAAGAGGAGGAAGAGGAAGAAGAGCC ACAGCAGCGAGGGTCAGGAGAGGAGCCAGCTACACCCTCGAGGAAGATCCTGGACCCTAACAGTGGGGAG CCAGCTCCCGTGCTGTCCTCCCCGCCTCCCACAGACCTGTCCACCTTCCTGTCATTCCCCTCCCCAGAGA AGCTGCTGCGGCTGGGTCCAAAGGTCTCGGTGCTGATAGTCCAGCAGACTGATACCTCCGACCCTGAGAA GGTTGTTTCAGCCTTCCTGAAGGTGGCATCTGTGTTCAGGGACGAAGCCTCAGTAAAGACAGCAGTGCTG GATGCCATCGATGCCCTCATGAAGAAGGCCTTCAGCTCCTCCTCTTTCAATTCCAACACCTTCCTTACCA GGCTGCTCATCCACATGGGTCTGCTCAAGAGTGAAGACAAGATCAAGGCCATTCCTAGCCTGCATGGCCC CCTGATGGTGTTGAACCACGTGGTACAGCAGGACTATTTCCCCAAGACCCTTGCACCGCTGCTGCTGGCG TTTGTGACAAAGCCCAATGGTGCACTGGAATCCTGCTCCTTTGCCCGCCACAGTCTGCTGCAGACGCTGT ACAGCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216957 representing NM_001244855 Red=Cloning site Green=Tags(s) MASEDIAKLAETLAKTQVAGGQLSFKGKGLKLNTAEDAKDVIKEIEEFDGLEALRLEGNTVGVEAARVIA KALEKKSKLKRCHWSDMFTGRLRSEIPPALISLGEGLITAGAQLVELDLSDNAFGPDGVRGFEALLKSPA CFTLQELKLNNCGMGIGGGKILAAALTECHRKSSAQGKPLTLKVFVAGRNRLENDGATALAEAFGIIGTL EEVHMPQNGINHPGVTALAQAFAINPLLRVINLNDNTFTEKGGVAMAETLKTLRQVEVINFGDCLVRSKG AVAIADAVRGGLPKLKELNLSFCEIKRDAALVVAEAVADKAELEKLDLNGNALGEEGCEQLQEVLDSFNM AKVLASLSDDEGEDEDEDEEGEDDEDEEDEEEEEEEEEEEEEEEEEPQQRGSGEEPATPSRKILDPNSGE PAPVLSSPPPTDLSTFLSFPSPEKLLRLGPKVSVLIVQQTDTSDPEKVVSAFLKVASVFRDEASVKTAVL DAIDALMKKAFSSSSFNSNTFLTRLLIHMGLLKSEDKIKAIPSLHGPLMVLNHVVQQDYFPKTLAPLLLA FVTKPNGALESCSFARHSLLQTLYSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001244855
ORF Size	1758 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244855.1, NP_001231784.1
RefSeq Size	2783 bp
RefSeq ORF	1761 bp
Locus ID	362965
MW	63.1 kDa

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