Hey2 (NM_130417) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Herp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216405 representing NM_130417 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGCCCTTGCGAGGAGACGACCTCTGAAAGCGACCTAGACGAGACCATCGACGTGGGGAGCGAGA ACAATTACTCCGGGCACAGTGCAAGCTCAGTGATGAGGTCCAATTCACCTACAACCACCTCTCAGATTAT GGCAAGAAAGAAAAGGAGAGGGATCATAGAAAAAAGGCGTCGGGATCGAATAAATAACAGTTTATCTGAA TTGAGAAGACTAGTGCCAACAGCTTTTGAAAAACAAGGATCTGCCAAGTTGGAAAAGGCTGAAATTCTGC AAATGACAGTGGATCATTTGAAGATGCTCCAGGCAACAGGGGGGAAAGGCTACTTTGACGCCCATGCCCT TGCCACGGACTTTATGAGCATTGGATTCCGAGAATGCTTGACAGAAGTGGCGAGGTACCTAAGCTCTGTG GAAGGCCTTGACCCTTCGGACCCACTACGCGTGCGCCTTGTCTCTCATCTCAGCACCTGTGCCTCCCAGC GGGAGGCAGCAGTGATGACATCCTCCATGTCCCACCACCATCACCCCCTGCACCCTCACCACTGGGCAGC GGCCTTCCACCATCTCCCCACATCCCTGCTCCAACCCAATGGACTCCACACATCAGAGTCAACGCCATGT CGCCTATCCACGTCTTCAGAAGTGCCTCCTGCTCATGGCTCTGCCCTCCTCACAGCAACATTTGCCCATG CAGATTCTGCTCTTCGGATGCCATCAACGGGCACCGTCGCACCCTGCGTGCCACCTCTCTCCACCTCTCT CCTGTCTCTTTCAGCTACTGTGCATGCCGCAGCAGCAGCGGCCACCGCAGCTGCACACAGCTTCCCTCTG TCCTTCGCAGGGGCCTTTCCCATGCTCCCATCCAATGCAGCAGCAGCCGCCGCGGTTGCCGCTGCAACAG CAATCAGCCCACCGTTATCAGTGTCTGCAGCCTCCAGTCCTCAGCAGACAAGCAGTGGAACAAACAGTAA ACCTTACCGACCCTGGGGGACAGAAGTTGGAGCCTTT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216405 representing NM_130417 Red=Cloning site Green=Tags(s) MKRPCEETTSESDLDETIDVGSENNYSGHSASSVMRSNSPTTTSQIMARKKRRGIIEKRRRDRINNSLSE LRRLVPTAFEKQGSAKLEKAEILQMTVDHLKMLQATGGKGYFDAHALATDFMSIGFRECLTEVARYLSSV EGLDPSDPLRVRLVSHLSTCASQREAAVMTSSMSHHHHPLHPHHWAAAFHHLPTSLLQPNGLHTSESTPC RLSTSSEVPPAHGSALLTATFAHADSALRMPSTGTVAPCVPPLSTSLLSLSATVHAAAAAATAAAHSFPL SFAGAFPMLPSNAAAAAAVAAATAISPPLSVSAASSPQQTSSGTNSKPYRPWGTEVGAF SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_130417
ORF Size	1017 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_130417.1, NP_569101.1
RefSeq Size	2421 bp
RefSeq ORF	1020 bp
Locus ID	155430
MW	35.9 kDa
Gene Summary	basic helix-loop-helix transcriptional repressor; primary target of Notch [RGD, Feb 2006]

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