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Ogdh (NM_001017461) Rat Tagged ORF Clone
CAT＃: RR215419
- TrueORF®
Ogdh (Myc-DDK-tagged ORF) - Rat oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_001017461" in other vectors (3)
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CNY 16,440.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR215419 representing NM_001017461
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTT CACAAAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGA ACCATTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAA AGTGTACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACC AGAGCCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCTCATGCACAGTCCCTGGTGGAAGCACA GCCTAACGTCGACAAGCTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCGTATCAGATACGA GGGCACCATGTAGCACAGCTGGACCCCCTGGGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTG ACATTATCTCATCCACAGACAAACTTGGGTTCTATGGCCTACACGAGTCTGACCTTGACAAGGTCTTCCA CTTACCCACCACCACTTTCATCGGGGGACAGGAGCCAGCACTTCCTCTTCGGGAGATCATCCGTCGGCTG GAGATGGCCTACTGCCAGCACATTGGTGTGGAGTTCATGTTCATTAACGATTTGGAGCAATGCCAGTGGA TCCGGCAGAAGTTTGAGACCCCTGGGATCATGCAGTTCACCAATGAGGAGAAGCGGACCCTGCTGGCCAG GCTTGTACGGTCCACCAGGTTTGAGGAGTTCCTGCAGCGAAAGTGGTCCTCTGAGAAGCGTTTTGGTCTG GAAGGCTGTGAGGTGCTGATCCCTGCCCTCAAGACAATCATTGACATGTCAAGTGCAAATGGAGTGGACT ATGTGATCATGGGGATGCCACACAGAGGACGACTGAACGTGCTAGCCAATGTCATCAGGAAGGAGCTGGA GCAGATATTCTGTCAGTTTGACTCGAAGCTGGAGGCAGCTGATGAGGGTTCCGGGGACATGAAGTACCAC CTGGGCATGTATCACCGAAGGATCAACCGTGTGACCGACAGAAACATCACTCTGTCCTTGGTGGCTAACC CTTCCCACTTAGAGGCTGCTGACCCTGTAGTGATGGGAAAGACCAAAGCTGAACAGTTCTACTGTGGAGA CACTGAAGGGAAAAAGGTGATGTCTATCCTGCTGCATGGGGATGCTGCCTTTGCTGGCCAGGGTATCGTG TATGAGACCTTCCATCTCAGCGACCTGCCATCCTATACAACCCACGGCACCGTTCACGTGGTTGTCAACA ACCAGATTGGCTTCACCACAGACCCTCGGATGGCCCGCTCTTCTCCCTACCCCACTGATGTGGCCCGAGT GGTGAACGCCCCCATTTTCCATGTCAACTCAGATGACCCTGAAGCTGTCATGTATGTATGCAAGGTGGCA GCTGAGTGGAGAAACACCTTCCACAAGGATGTTGTTGTTGATCTGGTGTGTTATCGACGAAATGGCCACA ACGAGATGGATGAACCTATGTTCACACAGCCACTCATGTACAAGCAGATCCGTAAGCAGAAGCCGGTACT GCAGAAGTATGCAGAATTGCTGGTATCCCAGGGGGTCGTCAACCAGCCCGAATATGAGGAGGAAATCTCC AAGTACGATAAGATCTGTGAGGAAGCATTTACCAGATCCAAAGATGAGAAGATCTTGCACATCAAGCACT GGCTGGATTCCCCCTGGCCTGGCTTTTTCACCCTGGATGGACAGCCCAGGAGCATGACCTGCCCCTCCAC TGGCCTGGAGGAGGACATCTTGACCCACATTGGGAATGTGGCCAGTTCTGTACCTGTGGAGAACTTTACC ATCCATGGAGGGCTGAGCCGGATCTTGAAGACTCGCAGAGAGCTTGTGACAAACCGGACTGTGGACTGGG CCCTGGCAGAGTACATGGCGTTCGGATCACTCCTGAAGGAAGGCATCCATGTCCGGCTGAGTGGCCAGGA CGTGGAGCGGGGTACCTTCAGCCATCGCCACCATGTGCTCCATGACCAGAATGTGGACAAAAGAACCTGC ATCCCCATGAACCACCTTTGGCCTAACCAGGCCCCTTACACCGTGTGCAACAGCTCGTTGTCTGAGTATG GTGTCCTGGGCTTTGAACTGGGCTTTGCCATGGCTAGCCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTT TGGTGATTTCAACAACATGGCACAGTGCATCATTGACCAGTTCATCTGCCCAGGACAGGCAAAGTGGGTG CGGCAGAATGGCATCGTGCTCCTGCTACCTCACGGCATGGAAGGCATGGGTCCCGAGCATTCCTCTGCCC GTCCAGAGAGGTTTCTGCAGATGTGCAATGATGACCCAGATGTCCTGCCTAACCTGCAGGAGGAAAACTT TGACATCAGTCAGCTCTACGACTGCAACTGGATTGTCGTCAACTGCTCCACCCCTGGCAACTTCTTCCAC GTGCTGCGACGACAGATCTTGCTGCCCTTCCGGAAGCCGTTAATCGTCTTCACTCCCAAATCCCTCCTGC GCCACCCTGAGGCAAGAACTAGCTTTGATGAGATGCTGCCAGGAACCCACTTCCAGCGTGTGATCCCAGA AGATGGACCTGCAGCTCAGAACCCAGACAAAGTCAAGAGGCTTCTCTTCTGCACTGGCAAGGTGTACTAT GACCTCACCCGAGAGCGCAAAGCCAGGGACATGGCAGAGGAGGTGGCTATTACAAGGATTGAGCAGCTGT CACCATTCCCCTTCGACCTCCTGCTGAAAGAGGCACAGAAGTATCCCAATGCTGAGCTGGCCTGGTGCCA GGAAGAGCACAAGAATCAAGGCTACTATGACTATGTCAAGCCAAGACTTCGGACCACCATTGACCGTGCT AAGCCTGTCTGGTATGCTGGCCGAGACCCAGCAGCCGCTCCAGCCACTGGCAACAAGAAGACCCACCTGA CAGAGCTGCAGCGCTTTCTGGACACAGCCTTTGACCTGGACGCCTTCAAGAAATTCTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215419 representing NM_001017461
Red=Cloning site Green=Tags(s) MFHLRTCAAKLRPLTASQTVKTFSQNKPAAIRTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPK SVHKSWDIFFRNTNAGAPPGTAYQSPLSLSRSSLATMAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIR GHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLHESDLDKVFHLPTTTFIGGQEPALPLREIIRRL EMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGL EGCEVLIPALKTIIDMSSANGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDMKYH LGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIV YETFHLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVA AEWRNTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEIS KYDKICEEAFTRSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLEEDILTHIGNVASSVPVENFT IHGGLSRILKTRRELVTNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRTC IPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFNNMAQCIIDQFICPGQAKWV RQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPNLQEENFDISQLYDCNWIVVNCSTPGNFFH VLRRQILLPFRKPLIVFTPKSLLRHPEARTSFDEMLPGTHFQRVIPEDGPAAQNPDKVKRLLFCTGKVYY DLTRERKARDMAEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTIDRA KPVWYAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001017461 |
| ORF Size | 3069 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001017461.1, NP_001017461.1 |
| RefSeq Size | 3266 bp |
| RefSeq ORF | 3072 bp |
| Locus ID | 360975 |
| UniProt ID | Q5XI78 |
| MW | 116.3 kDa |
| Gene Summary | 2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN215419 | Ogdh (untagged ORF) - Rat oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 16,440.00 |
|
| RR215419L3 | Lenti ORF clone of Ogdh (Myc-DDK-tagged ORF) - Rat oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 18,050.00 |
|
| RR215419L4 | Lenti ORF clone of Ogdh (mGFP-tagged ORF) - Rat oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 19,860.00 |
