Edn1 (NM_012548) Rat Tagged ORF Clone
CAT#: RR215408
- TrueORF®
Edn1 (Myc-DDK-tagged ORF) - Rat endothelin 1 (Edn1), (10 ug)
"NM_012548" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Et1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR215408 representing NM_012548
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTATTTTCCCGTGATCTTCTCTCTGCTGTTTGTGGCTTTCCAAGGAGCTCCAGAAACAGCTGTCT TGGGAGCAGAGCTCAGCCCCCGAGCTGAGAAGGAAGTGCAGAGCCCCCCTCCCAGCACATCCTGGAGACC CCGCAGGTCCAAGCGTTGCTCCTGCTCCTCCTTGATGGACAAGGAGTGTGTCTACTTCTGCCACCTGGAC ATCATCTGGGTCAACACTCCCGAGCGCGTCGTCCCGTATGGACTAGGAAGCCCTTCTAGGTCTAAGCGAT CCTTGAAAGACTTACTTCCCACAAAGACCACAGACCAAGGGAACAGATGCCAGTGTGCTCACCAAAAAGA CAAGAAGTGCTGGAATTTCTGCCAAGCAGACAAAGAACTCCGAGCCCAAAGTACCATGCAGAAAGGCGTA AAAGACTTCAAGAAGGGAAAACCCTGTCCCAAGCTGGGAAAGAAGTGTATCTATCAGCAGCTGGTGGAGG GAAGAAAACTAAGAAGGTTGGAGGCCATCAGCAACAGCATCAAGACCTCCTTTCGAGTTGCAAAGTTGAA AGCGGAACTCTACAGAGACCAGAAGTTGATACACAACCGAGCACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215408 representing NM_012548
Red=Cloning site Green=Tags(s) MDYFPVIFSLLFVAFQGAPETAVLGAELSPRAEKEVQSPPPSTSWRPRRSKRCSCSSLMDKECVYFCHLD IIWVNTPERVVPYGLGSPSRSKRSLKDLLPTKTTDQGNRCQCAHQKDKKCWNFCQADKELRAQSTMQKGV KDFKKGKPCPKLGKKCIYQQLVEGRKLRRLEAISNSIKTSFRVAKLKAELYRDQKLIHNRAH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012548 |
ORF Size | 606 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012548.2, NP_036680.1 |
RefSeq Size | 1390 bp |
RefSeq ORF | 609 bp |
Locus ID | 24323 |
UniProt ID | P22388 |
MW | 23.1 kDa |
Gene Summary | activates ERK1/2 mediated signaling; may play a role in regulation of cardiac contractile force [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN215408 | Edn1 (untagged ORF) - Rat endothelin 1 (Edn1), (10 ug) |
CNY 3,990.00 |
|
RR215408L3 | Lenti ORF clone of Edn1 (Myc-DDK-tagged ORF) - Rat endothelin 1 (Edn1), (10 ug) |
CNY 6,080.00 |
|
RR215408L4 | Lenti ORF clone of Edn1 (mGFP-tagged ORF) - Rat endothelin 1 (Edn1), (10 ug) |
CNY 6,650.00 |