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Npy (NM_012614) Rat Tagged ORF Clone
CAT＃: RR214992
- TrueORF®
Npy (Myc-DDK-tagged ORF) - Rat neuropeptide Y (Npy), (10 ug)
"NM_012614" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NPY02; RATNPY; RATNPY02 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214992 representing NM_012614
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTAGGTAACAAACGAATGGGGCTGTGTGGACTGACCCTCGCTCTATCCCTGCTCGTGTGTTTGG GCATTCTGGCTGAGGGGTACCCCTCCAAGCCGGACAATCCGGGCGAGGACGCGCCAGCAGAGGACATGGC CAGATACTACTCCGCTCTGCGACACTACATCAATCTCATCACCAGACAGAGATATGGCAAGAGATCCAGC CCTGAGACACTGATTTCAGATCTCTTAATGAGAGAAAGCACAGAAAATGCCCCCAGAACAAGGCTTGAAG ACCCTTCCATGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214992 representing NM_012614
Red=Cloning site Green=Tags(s) MMLGNKRMGLCGLTLALSLLVCLGILAEGYPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRYGKRSS PETLISDLLMRESTENAPRTRLEDPSMW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012614 |
| ORF Size | 294 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012614.2, NP_036746.1 |
| RefSeq Size | 567 bp |
| RefSeq ORF | 297 bp |
| Locus ID | 24604 |
| UniProt ID | P07808 |
| MW | 11 kDa |
| Gene Summary | This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. Studies in the rat model of depression (Flinders Sensitive Line) show that this gene is downregulated in the hippocampus and the prefrontal cortex compared to the control ((Flinders Resistant Line). Alternatively spliced transcript variants of this gene have been described, but the function of all the variants is not known. [provided by RefSeq, Jul 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214992 | Npy (untagged ORF) - Rat neuropeptide Y (Npy), (10 ug) |
CNY 3,990.00 |
|
| RR214992L3 | Lenti ORF clone of Npy (Myc-DDK-tagged ORF) - Rat neuropeptide Y (Npy), (10 ug) |
CNY 6,080.00 |
|
| RR214992L4 | Lenti ORF clone of Npy (mGFP-tagged ORF) - Rat neuropeptide Y (Npy), (10 ug) |
CNY 6,650.00 |
