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Gpi (NM_207592) Rat Tagged ORF Clone
CAT＃: RR214225
- TrueORF®
Gpi (Myc-DDK-tagged ORF) - Rat glucose phosphate isomerase (Gpi), (10 ug)
"NM_207592" in other vectors (3)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Amf; Gpi1; Nlk; Pgi; Phi |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214225 representing NM_207592
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGCTCACCCGGAACCCGGAGTTCCAGAAGCTGCTGGAGTGGCACCGGGCGAACTCTGCCAACC TCAAGCTGCGCGAACTTTTTGAGGCAGATCCGGAGCGCTTCAACCACTTCAGCTTGAACCTCAACACCAA CCATGGACATATTCTGTTGGATTACTCCAAGAACCTTGTGAACAAGGAGGTGTTGCACATGCTGGTGGAC CTGGCCAAGTCCAGAGGCGTGGAGGCCGCGCGGGACAACATGTTCAGTGGTTTGAAGATCAACTCCACCG AGGACCGGGCGGTGCTGCACGTGGCCCTTCGGAACCGGTCCAACAGATCCATCATGATGGACGGCAAAGA CGTGATGCCAGAGGTCAACAAGGTGCTGGACAAGATGAAGTCTTTCTGCCAGCGGGTCCGGAGTGGTGAC TGGAAAGGGTACACCGGCAAAGCTATCACGGACATCATCAACATCGGCATTGGAGGCTCCGACCTGGGAC CCCTCATGGTGACTGAAGCTCTCAAGCCTTACTCTAAAGGAGGTCCCAGAGTCTGGTTTGTCTCCAACAT TGATGGGACCCACATTGCCAAAACATTGGCCAACCTGAACCCTGAGTCTTCCCTCTTTATAATCGCCTCC AAGACCTTCACCACCCAGGAGACCATCACCAACGCAGAGACTGCCAAAGAGTGGTTTCTCCAAGCTGCGA AGGATCCGTCTGCAGTTGCAAAGCACTTTGTTGCCCTGTCTACGAACACGGACAAAGTGAAGGAGTTTGG AATTGACCCTAAAAACATGTTCGAGTTCTGGGATTGGGTAGGTGGCCGCTACTCGCTGTGGTCAGCCATT GGACTCTCCATCGCACTGCATGTGGGTTTTGACCACTTTGAACAGCTGCTGTCCGGGGCTCACTGGATGG ACCAGCACTTCATGAAGACGCCCCTGGATAAGAATGCCCCCGTCCTGCTGGCTCTCCTGGGCATCTGGTA TATCAACTTCTACGGCTGTGAGACCCACGCCATGCTGCCCTATGACCAGTACATGCACCGCTTTGCTGCC TATTTCCAGCAGGGTGACATGGAATCCAATGGAAAGTACATCACCAAGTCTGGAGCCCGGGTTGACTACC AGACAGGCCCCATTGTGTGGGGGGAGCCAGGGACCAACGGTCAACATGCATTCTACCAGCTTATCCACCA AGGCACCAAGATGATACCCTGTGATTTCCTCATCCCTGTTCAGACCCAGCACCCCATACGGAATGGTCTG CATCACAAGATCCTCCTGGCCAACTTCTTGGCCCAGACTGAGGCCCTGATGAAGGGGAAGTCGCCAGAAG AGGCCAGGAAGGAGCTCCAGGCTGCCGGAAAGAGCCCAGAAGAACTGGAGAAACTCTTGCCACACAAGGT CTTTGAAGGAAACCGGCCGACCAACTCAATTGTGTTTACCAAGCTAACACCCTTCATTCTGGGAGCACTG ATTGCCATGTATGAGCACAAGATCTTCGTTCAGGGCATCATCTGGGACATCAACAGCTTCGACCAGTGGG GAGTGGAGCTGGGGAAGCAGCTGGCCAAGAAAATTGAGCCAGAGCTGGACGGCAGCTCTGCCGTAACCTC CCATGACTCCTCCACTAATGGACTGATCGGCTTCATCAAGCTGCAGCGCGACACCAAAATAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214225 representing NM_207592
Red=Cloning site Green=Tags(s) MAALTRNPEFQKLLEWHRANSANLKLRELFEADPERFNHFSLNLNTNHGHILLDYSKNLVNKEVLHMLVD LAKSRGVEAARDNMFSGLKINSTEDRAVLHVALRNRSNRSIMMDGKDVMPEVNKVLDKMKSFCQRVRSGD WKGYTGKAITDIINIGIGGSDLGPLMVTEALKPYSKGGPRVWFVSNIDGTHIAKTLANLNPESSLFIIAS KTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTDKVKEFGIDPKNMFEFWDWVGGRYSLWSAI GLSIALHVGFDHFEQLLSGAHWMDQHFMKTPLDKNAPVLLALLGIWYINFYGCETHAMLPYDQYMHRFAA YFQQGDMESNGKYITKSGARVDYQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRNGL HHKILLANFLAQTEALMKGKSPEEARKELQAAGKSPEELEKLLPHKVFEGNRPTNSIVFTKLTPFILGAL IAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSSAVTSHDSSTNGLIGFIKLQRDTKID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_207592 |
| ORF Size | 1674 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_207592.1, NP_997475.1 |
| RefSeq Size | 2130 bp |
| RefSeq ORF | 1677 bp |
| Locus ID | 292804 |
| UniProt ID | Q6P6V0 |
| MW | 62.8 kDa |
| Gene Summary | This gene encodes a member of the glucose phosphate isomerase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214225 | Gpi (untagged ORF) - Rat glucose phosphate isomerase (Gpi), (10 ug) |
CNY 7,130.00 |
|
| RR214225L3 | Lenti ORF clone of Gpi (Myc-DDK-tagged ORF) - Rat glucose phosphate isomerase (Gpi), (10 ug) |
CNY 9,030.00 |
|
| RR214225L4 | Lenti ORF clone of Gpi (mGFP-tagged ORF) - Rat glucose phosphate isomerase (Gpi), (10 ug) |
CNY 9,980.00 |
