Rapgef2 (NM_001107684) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	nRap GEP; PDZ-GEF1; RA-GEF-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214025 representing NM_001107684 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACAGCTTTGGTGTTTCGCCCACCATGGACAAAGAGTACATGAAAGGAGTCATGAGGACAAAGG TGGATGACTGCCAGTTTGTCTGCATTGCCCAGCAAGATTACTGCCGTATTTTGAACCAAGTAGAAAAGAA CATGCAAAAAGTTGAGGAAGAAGGAGAGATTGTAATGGTGAAGGAGCACCGAGAACTTGATCGGACTGGA ACAAGGAAGGGCCACATCGTCATCAAGGGCACCTCAGAGAGGCTAACCATGCATTTGGTGGAAGAGCATT CTGTAGTGGACCCAACATTCATAGAAGACTTCCTGTTGACATACCGGACTTTCCTTTCCAGCCCAATGGA AGTGGGCAAGAAGTTATTGGAGTGGTTCAATGACCCGAGCCTCAGGGATAAGGTGACACGGGTAGTATTA CTGTGGGTGAATAATCACTTCAATGACTTTGAAGGAGATCCTGCAATGACTCGATTTCTAGAAGAATTTG AAAATAATCTGGAAAGAGAGAAAATGGGTGGACATCTAAGGCTGCTAAACATTGCATGTGCAGCGAAAGC CAAACGAAGGTCGATGACGTTAACAAAGCCGTCCCGGGAGGCGCCTCTGCCCTTCATCCTGCTGGGAGGC TCTGAAAAGGGCTTCGGCATCTTTGTGGACAGTGTTGACTCATCGAGCAAAGCCACTGAAGCAGGCTTGA AGCGTGGGGATCAGATATTAGAAGTGAATGGTCAAAATTTTGAAAATATCCAACTTTCAAAGGCAATGGA AATTCTTAGAAATAACACACACCTGTCCATCACTGTGAAAACCAACTTATTTGTATTTAAAGAACTTCTG ACGAGATTGTCTGAAGAGAAAAGAAATGGTGCCCCCCACCTTCCTAAAATTGGAGACATCAAGAAGGCCA GTCGCTACTCCATACCGGATCTTGCTGTAGATGTAGAACAGGTGATAGGCCTCGAGAAGGTCAACAAGAA AAGCAAAGCCAACACTGTGGGGGGAAGAAACAAGCTAAAGAAAATACTCGACAAGACTCGGATCAGTATC TTACCTCAGAAACCATACAATGATATTGGGATTGGTCAGTCTCAAGATGATAGCATCGTAGGACTGAGGC AGACCAAGCACATTCCAGCTGCCCTGCCTGTCAGTGGAACGCTGTCTTCCAGTAACCCTGACTTGCTGCA GTCACACCACCGGATTCTGGACTTCAGCACTACTCCTGACTTGCCAGATCAGGTACTGAGAGTTTTCAAG GCTGACCAGCAAAGCAGATACATCATGATCAGTAAGGACACCACAGCAAAGGAAGTGGTCATCCAGGCCA TCAGAGAGTTTGCTGTTACTGCCACCCCAGAGCAGTATTCCCTCTGTGAGGTCTCGGTCACGCCAGAGGG AGTTATCAAACAACGAAGGCTCCCAGATCAACTTTCCAAGCTTGCTGACAGAATACAGCTGAGCGGGAGG TATTATCTGAAAAACAACATGGAAACAGAAACGCTTTGCTCAGATGAAGATGCCCAGGAGCTGTTGCGAG AGAGCCAAATTTCACTTCTTCAGCTCAGCACTGTGGAGGTCGCCACACAGCTCTCCATGAGGAACTTTGA ACTCTTCCGCAACATTGAGCCTACTGAATACATAGATGATTTATTTAAACTCAAATCAAAAACCAGCTGT GCCAACCTAAAGAAATTTGAGGAAGTCATTAACCAGGAAACATTTTGGGTGGCTTCTGAAATTCTCCGAG AGACAAACCAGCTGAAGAGAATGAAGATCATTAAGCATTTCATCAAGATTGCCTTGCACTGTAGGGAATG CAAGAATTTTAACTCCATGTTTGCAATCATCAGTGGCCTAAACCTGGCACCAGTGGCAAGACTACGGACC ACCTGGGAAAAACTTCCCAATAAATATGAAAAGCTCTTCCAGGACCTACAAGACCTGTTTGATCCTTCCA GAAACATGGCTAAGTATCGCAATGTCCTGAGTGGCCAGAACTTACAGCCTCCTGTCATTCCCCTATTCCC CGTCATCAAGAAGGACCTCACGTTTCTCCATGAGGGAAATGACTCAAAAGTAGATGGGCTGGTCAACTTT GAGAAGCTGAGGATGATTGCAAAGGAAATCCGTCATGTTGGCCGAATGGCCTCAGTCAACATGGACCCTG CCCTTATGTTCAGAACTCGGTCGCTGAGTCAGGGCAGTGCGAATGCCACAGTGCTGGATGTTGCTCAGAC AGGCGGGCATAAAAAGCGGGTACGCCGTAGTTCCTTCCTCAATGCCAAAAAGCTGTATGAAGATGCCCAA ATGGCTCGGAAAGTGAAGCAATACCTGTCCAACCTGGAGCTGGAGATGGACGAGGAGAGTCTTCAGACAC TGTCACTTCAGTGTGAACCAGCAACCAGCACATTGCCCAAGAACCCTGCAGACAGGAAGCCTGTCAAGTC CGAGACCTCCCCAGTGGCTCCACGTGCAGGGCCCCAGCAGAAAGCGCAGCCTCAGCAGCCGTTGGCACCC CCTCAACCATCGCATAAAGTCAGCCAGGGGCTGCAGGTGCCTGCTGTGTCCCTCTACCCCTCACGAAAGA AGGTGCCAGTGAAGGATCTGCCGCCCTTTGGCATAAACTCTCCACAAGCTTTAAAGAAAATCCTGTCCTT GTCTGAAGAAGGGAGTTTGGAGCGTCATAGGAAGCAAGCAGAAGACACAATATCAAACGCATCCTCACAG CTCTCATCACCCCCCACCTCACCTCAGAGCTCTCCAAGGAAAGGGTATGCATTGGCTCTCAGTGGGACTG TGGATAATTTCTCAGATTCTGGTCACAGTGAGATTTCTTCACGATCCAGTATTGTCAGCAATTCTTCTTT TGACTCGGTGCCAGTCTCTCTGCATGATGAACGGCGCCAGAGGCATTCTGTCAGCATTGTGGAGAGCAAC CTAGGGGTGGGCAGGATGGAGCGGCGGACCCTGATGGAGCCTGATCAATACAGCCTAGGATCATATGCCC CAGTGTCTGAAAGTCGAGGCCTGTATGCCGCAGCGACAGTCATCTCTTCTCCTAGCACAGAGGAGCTTTC CCACGACCAAGGGGACCGTGCTTCTCTCGATGCTGCTGACAGCGGTCGGGGGAGCTGGACATCATGCTCA AGTGGTTCTCATGACAACATACAGACCATCCAGCACCAGAGAAGCTGGGAAACACTGCCATTTGGGCACA CTCACTTTGACTATTCAGGGGATGCGACAGGTATATGGGCCTCCGGCGGCCATATGGACCAAATCATGTT TTCTGATCATAGCACAAAATATAATAGACAGAATCAGAGTAGAGAGAGTCTTGAACAAGCCCAATCCCGG GCAAGCTGGGCGTCTTCCACGGGCTACTGGGGAGAAGACTCAGAAGGCGACACGGGCACTATCAAGCGAA GGGGTGGGAAGGATGTCTCCACTGAGGCAGAGAGCAGCAGCATGGTGCCTGTGACTACAGAGGAAGCTAA GCCTGTCCCTATGCCTGCCCACATAGCCGTGACGCCCAGCACTACCAAGGGACTCATCGCACGAAAGGAA GGCAGGTACCGGGAGCCGCCTCCCACACCTCCAGGCTACGTGGGCATTCCCATTGCCGATTTCCCAGAAG GGCCTTGCCACCCCGCCAGGAAGCCCCCGGATTACAACGTGGCCCTGCAGCGGTCCCGCATGGTGGCACG GCCCACTGAGGCCCCCGCACCAGGCCAGACGCCGCCGGCAGCCACAGCCAGCCGGCCGGGCAGCAAACCG CAGTGGCACAAGCCCAGCGACGCAGACCCACGCCTCGCGCCCTTCCAGCCGCAGGGCTTCGCCGGAGCGG AGGAGGACGAAGATGAACAAGTGTCTGCTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214025 representing NM_001107684 Red=Cloning site Green=Tags(s) MGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTG TRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVL LWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRSMTLTKPSREAPLPFILLGG SEKGFGIFVDSVDSSSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELL TRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISI LPQKPYNDIGIGQSQDDSIVGLRQTKHIPAALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFK ADQQSRYIMISKDTTAKEVVIQAIREFAVTATPEQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGR YYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSC ANLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRT TWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLSGQNLQPPVIPLFPVIKKDLTFLHEGNDSKVDGLVNF EKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSANATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQ MARKVKQYLSNLELEMDEESLQTLSLQCEPATSTLPKNPADRKPVKSETSPVAPRAGPQQKAQPQQPLAP PQPSHKVSQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRKQAEDTISNASSQ LSSPPTSPQSSPRKGYALALSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVESN LGVGRMERRTLMEPDQYSLGSYAPVSESRGLYAAATVISSPSTEELSHDQGDRASLDAADSGRGSWTSCS SGSHDNIQTIQHQRSWETLPFGHTHFDYSGDATGIWASGGHMDQIMFSDHSTKYNRQNQSRESLEQAQSR ASWASSTGYWGEDSEGDTGTIKRRGGKDVSTEAESSSMVPVTTEEAKPVPMPAHIAVTPSTTKGLIARKE GRYREPPPTPPGYVGIPIADFPEGPCHPARKPPDYNVALQRSRMVARPTEAPAPGQTPPAATASRPGSKP QWHKPSDADPRLAPFQPQGFAGAEEDEDEQVSAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107684
ORF Size	3882 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107684.1, NP_001101154.1
RefSeq Size	6311 bp
RefSeq ORF	3885 bp
Locus ID	310533
UniProt ID	F1M386
MW	144.3 kDa
Gene Summary	Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.[UniProtKB/Swiss-Prot Function]

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