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Rbm5 (NM_001100548) Rat Tagged ORF Clone
CAT＃: RR213857
- TrueORF®
Rbm5 (Myc-DDK-tagged ORF) - Rat RNA binding motif protein 5 (Rbm5), (10 ug)
"NM_001100548" in other vectors (5)
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CNY 8,952.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213857 representing NM_001100548
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTCAGACAAAAGAGTGAGTAGAACAGAGCGTAGTGGAAGATATGGTTCCATCATAGACAGGGATG ACCGTGACGAGCGTGAATCTAGAAGCAGGCGGAGGGACTCAGATTACAAAAGATCAAGTGATGATCGGAG AGGCGATAGATATGATGACTATCGAGACTATGATAGTCCGGAGAGAGAACGTGAAAGAAGGAACAGTGAC CGGTCTGAGGATGGCTACCATTCAGATGGTGACTATGGAGAACATGACTATAGACATGACATCAGTGACG AGAGAGAGAGCAAGACCATCATGCTCCGTGGCCTTCCTATCACCATCACCGAGAGCGATATTCGAGAAAT GATGGAGTCCTTTGAAGGCCCTCAGCCTGCAGATGTGAGGCTGATGAAGAGGAAAACAGGTGTAAGCCGT GGTTTCGCCTTCGTGGAGTTTTATCACTTGCAAGATGCTACCAGCTGGATGGAAGCCAATCAGAAAAAAT TGGTGATTCAAGGAAAGCACATTGCAATGCATTATAGCAATCCCAGACCTAAGTTTGAAGATTGGCTTTG TAACAAGTGCTGCCTTAACAATTTCAGGAAAAGACTAAAATGCTTCCGATGTGGAGCAGACAAGTTTGAC TCTGAACAGGAAGTGCCCCCTGGAACCACAGAGTCTGTGCAGTCTGTGGACTACTACTGTGACACGATCA TCCTTCGGAACATAGCTCCGCATACTGTGGTGGACTCCATCATGACAGCACTGTCTCCCTATGCTTCCTT AGCTGTCAATAACATTCGCCTCATAAAAGACAAACAGACACAACAGAACAGAGGCTTTGCCTTTGTGCAG CTATCTTCTGCGATGGATGCCTCTCAGCTGCTTCAGATATTGCAGAGTCTCCATCCACCATTGAAAATTG ATGGGAAAACTATTGGAGTTGACTTTGCAAAAAGTGCCAGAAAAGATTTGGTCCTTCCAGATGGTAACCG TGTCAGTGCCTTCTCTGTAGCTAGTACAGCCATCGCTGCTGCTCAGTGGTCATCCACTCAGTCTCAAAGT GGTGAAGGAGGCAATGTTGACTACAGTTACATGCAACCAGGCCAGGATGGCTATACACAGTACACTCAAT ACTCACAGGATTACCAGCAGTTTTATCAACAACAGACTGGAGGACTGGAGTCTGATGCATCAGCTACATC AGGTACCACGGTGACTACCACCTCAGCAGCTGTAGTATCCCAGAGTCCTCAACTCTATAATCAGACCTCC AATCCACCTGGCTCTCCGACTGAGGAAGCACAGCCTAGCACTAGCACAAGTACACAGGCCCCAGCTGCTT CCCCTACTGGTGTAGTTCCTGGTACCAAGTATGCGGTACCTGACACGTCTACTTATCAATATGATGAATC TTCAGGATATTATTATGATCCTACAACAGGGCTCTACTATGACCCTAACTCACAGTACTACTATAATTCC TTAACACAGCAGTACTTGTACTGGGATGGAGAGAAGGAGACCTACGTGCCAGCTGCAGAGTCTAGCTCTA ACCAACAAGCTGGCCTACCTTCCACAAAAGAGGGAAAGGAGAAGAAAGAAAAGCCCAAGAGCAAAACTGC TCAACAGATTGCCAAAGACATGGAACGCTGGGCTAAGAGTTTAAATAAGCAGAAAGAAAATTTTAAAAAC AGCTTTCAACCTGTCAATTCATTGAGAGAAGAAGAAAGGAGAGAATCTGCTGCAGCAGATGCTGGCTTTG CTCTTTTTGAGAAGAAGGGAGCCTTAGCTGAAAGGCAGCAACTCATCCCTGAATTGGTGCGAAATGGAGA TGAGGAAAATCCCCTCAAAAGAGGTCTGGTTGCAGCTTACAGTGGTGACAGTGACAATGAGGAAGAGCTG GTAGAGAGACTTGAGAGTGAGGAAGAGAAACTAGCTGACTGGAAGAAGATGGCCTGCCTGCTGTGCCGAC GGCAGTTCCCAAACAGAGATGCCCTGGTCAGGCACCAGCAGCTCTCTGATTTACACAAGCAAAATATGGA CATCTACCGAAGATCCAGGCTGAGCGAGCAGGAGTTGGAAGCCCTGGAGCTGAGAGAGAGAGAGATGAAA TACCGAGATCGAGCAGCAGAAAGACGAGAGAAATATGGAATTCCAGAGCCCCCAGAGCCCAAGCGCAAGA AACAGTTCGATGCTGGCACTGTGAATTACGAGCAGCCCACCAAAGATGGCATTGACCACAGTAACATTGG CAACAAGATGCTGCAGGCTATGGGCTGGCGGGAAGGCTCAGGCTTAGGAAGAAAGTGTCAAGGCATCACG GCTCCCATTGAGGCTCAAGTCCGGCTAAAAGGAGCTGGCTTAGGAGCCAAAGGCAGTGCATATGGGTTGT CAGGTGCCGATTCCTACAAAGATGCTGTTCGGAAAGCCATGTTTGCCCGGTTCACTGAGATGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213857 representing NM_001100548
Red=Cloning site Green=Tags(s) MGSDKRVSRTERSGRYGSIIDRDDRDERESRSRRRDSDYKRSSDDRRGDRYDDYRDYDSPERERERRNSD RSEDGYHSDGDYGEHDYRHDISDERESKTIMLRGLPITITESDIREMMESFEGPQPADVRLMKRKTGVSR GFAFVEFYHLQDATSWMEANQKKLVIQGKHIAMHYSNPRPKFEDWLCNKCCLNNFRKRLKCFRCGADKFD SEQEVPPGTTESVQSVDYYCDTIILRNIAPHTVVDSIMTALSPYASLAVNNIRLIKDKQTQQNRGFAFVQ LSSAMDASQLLQILQSLHPPLKIDGKTIGVDFAKSARKDLVLPDGNRVSAFSVASTAIAAAQWSSTQSQS GEGGNVDYSYMQPGQDGYTQYTQYSQDYQQFYQQQTGGLESDASATSGTTVTTTSAAVVSQSPQLYNQTS NPPGSPTEEAQPSTSTSTQAPAASPTGVVPGTKYAVPDTSTYQYDESSGYYYDPTTGLYYDPNSQYYYNS LTQQYLYWDGEKETYVPAAESSSNQQAGLPSTKEGKEKKEKPKSKTAQQIAKDMERWAKSLNKQKENFKN SFQPVNSLREEERRESAAADAGFALFEKKGALAERQQLIPELVRNGDEENPLKRGLVAAYSGDSDNEEEL VERLESEEEKLADWKKMACLLCRRQFPNRDALVRHQQLSDLHKQNMDIYRRSRLSEQELEALELREREMK YRDRAAERREKYGIPEPPEPKRKKQFDAGTVNYEQPTKDGIDHSNIGNKMLQAMGWREGSGLGRKCQGIT APIEAQVRLKGAGLGAKGSAYGLSGADSYKDAVRKAMFARFTEME TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100548 |
| ORF Size | 2445 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100548.1, NP_001094018.1 |
| RefSeq Size | 3018 bp |
| RefSeq ORF | 2448 bp |
| Locus ID | 300996 |
| UniProt ID | B2GV05 |
| MW | 92.8 kDa |
| Gene Summary | Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate apoptosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes production of a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes production of a catalytically active form of CASP2/Caspase-2 that induces apoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213857 | Rbm5 (untagged ORF) - Rat RNA binding motif protein 5 (Rbm5), (10 ug) |
CNY 10,450.00 |
|
| RR213857L1 | Lenti ORF clone of Rbm5 (Myc-DDK-tagged ORF) - Rat RNA binding motif protein 5 (Rbm5), (10 ug) |
CNY 11,352.00 |
|
| RR213857L2 | Lenti ORF clone of Rbm5 (mGFP-tagged ORF) - Rat RNA binding motif protein 5 (Rbm5), (10 ug) |
CNY 13,400.00 |
|
| RR213857L3 | Lenti ORF clone of Rbm5 (Myc-DDK-tagged ORF) - Rat RNA binding motif protein 5 (Rbm5), (10 ug) |
CNY 12,260.00 |
|
| RR213857L4 | Lenti ORF clone of Rbm5 (mGFP-tagged ORF) - Rat RNA binding motif protein 5 (Rbm5), (10 ug) |
CNY 13,400.00 |
