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Cyld (NM_001017380) Rat Tagged ORF Clone
CAT＃: RR213203
- TrueORF®
Cyld (Myc-DDK-tagged ORF) - Rat cylindromatosis (turban tumor syndrome) (Cyld), (10 ug)
"NM_001017380" in other vectors (3)
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CNY 12,260.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LRRGT00003; Rp1; Rp1h |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213203 representing NM_001017380
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCAGGCCTGTGGAACCAAGAGAAAGTTACTTCACCCTACTGGGAAGAACGGCTTTTTTATCTGC TTCTTCAAGAATGCAGTGTAACAGACAAACAGACACAGAAGCTCCTGAGAGTACCCAAAGGGAGCATAGG ACAGTACATCCAAGACCGTTCCGTGGGGCATTCAAGAGTTCCTTCTGCTAAAGGCAAGAAAAATCAGATT GGATTAAAAATCTTAGAGCAACCGCATGCAGTTCTGTTTGTTGATGAAAAGGATGTTGTAGAAATAAATG AAAAATTCACAGAGTTACTGTTGGCAATTACCAACTGTGAGGAGAGGCTCAGCCTATTTAGAAACAGAAT CCGACTAAGTAAAGGCCTCCAGGTAGACGTGGGCAGTCCTGTGAGAGTACAGCTGCGATCTGGGGAGGAG AAGTTTCCAGGAGTTGTACGCTTCAGAGGACCTTTATTAGCGGAGAGGACGGTGTCGGGGATTTTCTTTG GAGTAGAATTACTGGAAGAAGGTCGTGGCCAAGGTTTCACTGATGGGGTGTATCAAGGAAAACAGCTCTT CCAGTGTGATGAGGACTGTGGCGTTTTTGTTGCATTGGACAAGCTGGAGCTTATAGAAGATGATGACAAT GGGTTGGAAAGTGATTTTGCAGGCCCAGGAGATACAGTCCAGGTTGAACCTCCCCCTTTGGAAATAAACT CCAGAGTTTCTTTGAAGGTTGGAGAAAGTACAGAATCTGGAACAGTGATATTCTGTGATGTTTTACCAGG AAAAGAGAGTCTAGGATATTTTGTTGGTGTGGACATGGATAACCCTATTGGCAACTGGGACGGAAGGTTT GATGGGGTTCAGCTTTGCAGTTTTGCAAGTGTTGAGAGTACAGTTCTCCTACACATCAATGACATCATCC CAGATAGCGTGACACAGGAAAGGAGACCTCCCAAACTTGCCTTTATGTCAAGAGGTGTAGGTGACAAAGG TTCATCTAGTCATAATAAACCAAAGGTTACAGGATCTACCTCAGACCCTGGAAGTAGAAACAGATCTGAA TTATTTTATACCTTAAATGGGTCATCTGTTGACTCACAACAACAATCCAAGTCCAAAAACCCATGGTACA TTGATGAAGTTGCAGAAGACCCTGCAAAGTCACTTACAGAGATGTCTTCAGACTTCGGACATTCATCGCC TCCACCGCAACCTCCTTCCATGAACTCCTTGTCTAGCGAGAACAGATTCCACTCCTTACCCTTCAGCCTG ACAAAGATGCCCAACACTAATGGCAGCATGGCTCACAGTCCACTCTCTCTGTCAGTGCAGTCTGTGATGG GAGAGCTGAACAGCACTCCTGTCCAGGAGAGTCCACCCATGCCCAGCTCTTCTGGGAATGCACACGGGCT AGAGGTGGGCTCACTGGCTGAAGTAAAAGAGAACCCCCCGTTCTATGGGGTTATCCGTTGGATTGGCCAG CCACCAGGGCTCAGTGACGTGCTTGCTGGATTGGAACTGGAAGATGAATGCGCAGGTTGTACGGATGGAA CTTTCAGGGGCACGCGCTATTTCACCTGTGCCCTGAAGAAAGCACTGTTCGTGAAACTGAAGAGCTGCAG ACCAGACTCTAGGTTTGCATCCTTGCAGCCTGTTTCCAATCAGATCGAAAGGTGTAACTCTTTAGCATTT GGGGGCTACTTAAGTGAAGTAGTAGAAGAAAATACGCCACCTAAAATGGAAAAGGAAGGTTTAGAGATAA TGATTGGAAAGAAGAAAGGCATCCAGGGCCATTACAATTCTTGTTACTTAGACTCAACTTTATTCTGCTT ATTTGCTTTTAGTTCTGCCCTGGACACTGTATTACTTAGACCCAAAGAGAAGAATGATGTAGAGTATTAC AGTGAGACTCAAGAGCTACTGAGGACAGAGATAGTCAATCCTCTGAGAATATATGGATATGTGTGTGCCA CAAAGATTATGAAGCTGAGGAAAATACTTGAAAAAGTTGAGGCTGCATCAGGATTTACCTCTGAGGAAAA AGATCCTGAAGAATTTCTAAACATCCTGTTTCATGATATTTTAAGGGTTGAACCATTGTTAAAAATAAGG TCAGCAGGTCAAAAAGTTCAAGACTGTAACTTCTATCAAATTTTTATGGAAAAAAATGAGAAAGTCGGAG TACCCACAATCCAGCAGTTATTAGAATGGTCTTTTATCAACAGCAACCTGAAATTTGCAGAGGCACCATC ATGCTTGATTATCCAGATGCCTCGGTTTGGGAAAGACTTTAAACTATTTAAAAAAATTTTTCCTTCCCTG GAATTAAATATAACAGATTTACTTGAAGACACTCCCAGGCAGTGCCGCATCTGTGGAGGACTCGCCATGT ATGAGTGTAGAGAGTGCTATGATGACCCGGACATCTCGGCAGGGAAGATCAAGCAGTTCTGTAAGACCTG CAGCACTCAGGTTCACCTTCATCCCAGAAGACTGAATCACACTTACCATCCAGTATCACTTCCCAAAGAC TTGCCCGACTGGGACTGGAGACACGGCTGCATCCCGTGTCAGAAGATGGAGTTATTTGCTGTGCTCTGCA TAGAAACCAGCCACTATGTTGCTTTTGTGAAGTACGGGAAGGATGACTCTGCCTGGCTCTTCTTTGACAG CATGGCTGATCGAGATGGTGGTCAGAATGGCTTCAACATTCCACAAGTGACACCCTGCCCAGAAGTAGGA GAGTACTTGAAGATGTCTCTGGAGGACCTGCACTCTTTGGACTCCAGAAGGATTCAAGGCTGTGCGCGCA GACTTCTTTGCGATGCATACATGTGCATGTACCAGAGTCCAACCATGAGCTTGTACAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213203 representing NM_001017380
Red=Cloning site Green=Tags(s) MSSGLWNQEKVTSPYWEERLFYLLLQECSVTDKQTQKLLRVPKGSIGQYIQDRSVGHSRVPSAKGKKNQI GLKILEQPHAVLFVDEKDVVEINEKFTELLLAITNCEERLSLFRNRIRLSKGLQVDVGSPVRVQLRSGEE KFPGVVRFRGPLLAERTVSGIFFGVELLEEGRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDN GLESDFAGPGDTVQVEPPPLEINSRVSLKVGESTESGTVIFCDVLPGKESLGYFVGVDMDNPIGNWDGRF DGVQLCSFASVESTVLLHINDIIPDSVTQERRPPKLAFMSRGVGDKGSSSHNKPKVTGSTSDPGSRNRSE LFYTLNGSSVDSQQQSKSKNPWYIDEVAEDPAKSLTEMSSDFGHSSPPPQPPSMNSLSSENRFHSLPFSL TKMPNTNGSMAHSPLSLSVQSVMGELNSTPVQESPPMPSSSGNAHGLEVGSLAEVKENPPFYGVIRWIGQ PPGLSDVLAGLELEDECAGCTDGTFRGTRYFTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSLAF GGYLSEVVEENTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSALDTVLLRPKEKNDVEYY SETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHDILRVEPLLKIR SAGQKVQDCNFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSL ELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCSTQVHLHPRRLNHTYHPVSLPKD LPDWDWRHGCIPCQKMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVG EYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001017380 |
| ORF Size | 2859 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001017380.1, NP_001017380.1 |
| RefSeq Size | 3267 bp |
| RefSeq ORF | 2862 bp |
| Locus ID | 312937 |
| UniProt ID | Q66H62 |
| MW | 106.7 kDa |
| Gene Summary | Deubiquitinase that specifically cleaves 'Lys-63'- and linear 'Met-1'-linked polyubiquitin chains and is involved in NF-kappa-B activation and TNF-alpha-induced necroptosis. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation (By similarity). Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis. Involved in the regulation of ciliogenesis, allowing ciliary basal bodies to migrate and dock to the plasma membrane; this process does not depend on NF-kappa-B activation (By similarity). Ability to remove linear ('Met-1'-linked) polyubiquitin chains regulates innate immunity and TNF-alpha-induced necroptosis: recruited to the LUBAC complex via interaction with SPATA2 and restricts linear polyubiquitin formation on target proteins. Regulates innate immunity by restricting linear polyubiquitin formation on RIPK2 in response to NOD2 stimulation (By similarity). Involved in TNF-alpha-induced necroptosis by removing linear ('Met-1'-linked) polyubiquitin chains from RIPK1, thereby regulating the kinase activity of RIPK1 (By similarity). Removes 'Lys-63' linked polyubiquitin chain of MAP3K7, which inhibits phosphorylation and blocks downstream activation of the JNK-p38 kinase cascades (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213203 | Cyld (untagged ORF) - Rat cylindromatosis (turban tumor syndrome) (Cyld), (10 ug) |
CNY 12,260.00 |
|
| RR213203L3 | Lenti ORF clone of Cyld (Myc-DDK-tagged ORF) - Rat cylindromatosis (turban tumor syndrome) (Cyld), (10 ug) |
CNY 13,970.00 |
|
| RR213203L4 | Lenti ORF clone of Cyld (mGFP-tagged ORF) - Rat cylindromatosis (turban tumor syndrome) (Cyld), (10 ug) |
CNY 15,390.00 |
