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Nuak2 (NM_001007617) Rat Tagged ORF Clone
CAT＃: RR212466
- TrueORF®
Nuak2 (Myc-DDK-tagged ORF) - Rat NUAK family, SNF1-like kinase, 2 (Nuak2), (10 ug)
"NM_001007617" in other vectors (3)
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CNY 8,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1200013b22rik; Snark; UV126 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212466 representing NM_001007617
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGGTGGCCTTACACCGGCGCGGGAACCTGGCTCCCTCGGCCTCCGCCCTGGCCACGGAGAGCG CCCGGCCGCTGGCGGACCGGCTCATCAAGTCGCCCAAACCTCTGATGAAGAAGCAGGCGGTGAAGCGGCA CCATCACAAACACAACCTGAGGCACCGCTACGAGTTCCTGGAGACCCTGGGCAAGGGCACCTACGGGAAG GTGAAGAAAGCACGAGAGAGCTCGGGACGCCTGGTGGCCATCAAGTCTATCAGGAAGGACAAAATCAAAG ATGAGCAGGATCTGTTGCACATAAGGAGGGAGATCGAGATCATGTCTTCACTCAACCACCCCCACATCAT TGCCATCCATGAAGTGTTTGAGAACAGCAGCAAGATTGTGATTGTCATGGAGTACGCCAGCCGAGGCGAT CTGTACGATTACATCAGTGAGCGGCCACGGCTGAATGAGCGGGACGCCAGGCATTTCTTCCGACAGATCG TGTCCGCCCTGCACTACTGCCACCAGAACGGGATTGTTCACCGGGACCTCAAGCTGGAGAACATCCTTCT AGATGCCAGTGGCAACATCAAGATTGCTGATTTTGGCCTCTCCAACCTGTATCACAAAGGCAAGTTCCTC CAGACGTTCTGTGGGAGCCCTCTCTATGCCTCACCTGAGATCGTCAACGGGAAGCCCTATGTGGGCCCAG AGGTGGACAGCTGGTCTCTGGGCGTTCTTCTGTACATCCTGGTGCATGGCACCATGCCCTTTGACGGGCA GGATCATAAAACCCTGGTGAAACAAATCAGTAGCGGGGCTTACCGAGAGCCGTGCAAACCGTCTGATGCC TGTGGCCTGATCCGGTGGCTGTTAATGGTGAATCCCATCCGTCGGGCCACTCTGGAGGATGTAGCCAGTC ATTGGTGGGTCAACTGGGGTTACAGCACCCGAATTGGGGAACAGGAAGCTCTGCGAGAGGGTGGGCACCC TAGCGGTGACTCTGGCCGGGCCTCTATGGCGGACTGGTTACGTCGCTCCTCCCGCCCCCTCCTGGAGAAT GGAGCCAAAGTGTGTAGCTTCTTCAAGCAGCATGTGCCGGGAGGTGGAAGCACGGGACCGGGGCTGGAGC GGCAACATTCTCTTAAGAAGTCCCGCAAGGAGAATGACATGGCTCAGACTCTGCAGAATGACCCAGCTGA AGATACTTCCTCTCGCCCTGGCAAGAACAGCCTCAAGCTTCCGAAAGGTATCCTCAAGAAAAAGGCCTCT CCCTCATCGGGGGAGGTACAGGAGGGCCCTCAGGAACTCAGACCAGTGTCCAATACCCCAGGGCAGCCTG TCCCTGCTATACCCCTGCTCCCAAGGAAGGGCATTCTTAAGAAGTCTCGGCAGCGTGAATCTGGTTACTA CTCCTCTCCAGAGCCCAGTGAGTCTGGGGAACTCTTAGACGCAGGTGATGTGTTTGTGAGTGGGGACCCC ATGGAGCAGAAGTCTCCACAAGCTTCAGGGCGCCTCCATCGCAAGGGCATCCTCAAACTCAATGGCAAGT TTTCCCGCACAGCCTTAGAAGGCACTGCCCCTAGCACCTTTGGCTCCCTAGACCAACTGGCCTCCCCTCA TCCTACAGCCCGGGCCAGCCGTCCCTCGGGAGCTGTGAGTGAGGACAGCATCCTGTCCTCCGAGTCCTTT GACCAATTGGACTTGCCCGAGCGGCTTCCCGAAACCCCACTGAGGGGCTGTGTGTCTGTGGACAACCTGA GGAGGCTTGAGCAGCCTCCCTCAGAAGGCCTAAAACGATGGTGGCAGGAATCCTTGGGGGATAGCTGCTT TTCTCTGACAGACTGCCAAGAGGTGACAGCAGCCTACAGACAAGCCCTAGGAATCTGCTCGAAGCTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212466 representing NM_001007617
Red=Cloning site Green=Tags(s) MESVALHRRGNLAPSASALATESARPLADRLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGK VKKARESSGRLVAIKSIRKDKIKDEQDLLHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGD LYDYISERPRLNERDARHFFRQIVSALHYCHQNGIVHRDLKLENILLDASGNIKIADFGLSNLYHKGKFL QTFCGSPLYASPEIVNGKPYVGPEVDSWSLGVLLYILVHGTMPFDGQDHKTLVKQISSGAYREPCKPSDA CGLIRWLLMVNPIRRATLEDVASHWWVNWGYSTRIGEQEALREGGHPSGDSGRASMADWLRRSSRPLLEN GAKVCSFFKQHVPGGGSTGPGLERQHSLKKSRKENDMAQTLQNDPAEDTSSRPGKNSLKLPKGILKKKAS PSSGEVQEGPQELRPVSNTPGQPVPAIPLLPRKGILKKSRQRESGYYSSPEPSESGELLDAGDVFVSGDP MEQKSPQASGRLHRKGILKLNGKFSRTALEGTAPSTFGSLDQLASPHPTARASRPSGAVSEDSILSSESF DQLDLPERLPETPLRGCVSVDNLRRLEQPPSEGLKRWWQESLGDSCFSLTDCQEVTAAYRQALGICSKLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001007617 |
| ORF Size | 1890 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007617.1, NP_001007618.1 |
| RefSeq Size | 2900 bp |
| RefSeq ORF | 1893 bp |
| Locus ID | 289419 |
| UniProt ID | Q66HE5 |
| MW | 70 kDa |
| Gene Summary | Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212466 | Nuak2 (untagged ORF) - Rat NUAK family, SNF1-like kinase, 2 (Nuak2), (10 ug) |
CNY 8,080.00 |
|
| RR212466L3 | Lenti ORF clone of Nuak2 (Myc-DDK-tagged ORF) - Rat NUAK family, SNF1-like kinase, 2 (Nuak2), (10 ug) |
CNY 9,980.00 |
|
| RR212466L4 | Lenti ORF clone of Nuak2 (mGFP-tagged ORF) - Rat NUAK family, SNF1-like kinase, 2 (Nuak2), (10 ug) |
CNY 10,830.00 |
