Myo5a (NM_022178) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	D; Dop; Myh12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212159 representing NM_022178 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGTCCGAGCTCTACACCAAGTTTGCCAGGGTTTGGATCCCTGATCCTGAGGAAGTGTGGAAAT CGGCAGAGTTGCTCAAGGATTATAAGCCTGGAGATAAAGTGCTCCTGCTTCACCTTGAGGAAGGGAAGGA TTTGGAATACCGTCTAGATCCAAAGACCAGCGAGCTCCCTCACCTACGGAACCCTGACATACTTGTTGGA GAAAATGACCTCACAGCCCTCAGCTACCTTCACGAGCCTGCTGTGCTGCACAATCTCCGCGTCCGCTTCA TCGACTCCAAACTCATTTATACGTATTGTGGGATAGTCCTGGTAGCTATAAATCCCTATGAGCAGCTGCC TATCTATGGAGAAGATATCATTAATGCATACAGTGGCCAGAACATGGGTGACATGGACCCTCACATCTTT GCAGTAGCTGAAGAGGCCTATAAACAAATGGCAAGGGACGAACGAAATCAGTCCATCATTGTAAGTGGAG AGTCAGGTGCAGGGAAGACAGTCTCGGCTAAGTATGCCATGAGGTACTTCGCAACTGTAAGTGGCTCTGC CAGTGAGGCCAATGTTGAGGAAAAGGTCTTGGCCTCCAACCCCATCATGGAGTCAATTGGAAATGCTAAA ACAACCAGGAATGATAATAGCAGCCGGTTTGGGAAATATATTGAAATTGGTTTTGACAAGAGATATCGAA TCATCGGTGCCAATATGAGAACTTACCTTTTAGAGAAATCCAGAGTGGTGTTCCAGGCAGAAGAGGAGAG AAACTACCATATCTTCTATCAGCTCTGTGCTTCGGCAAAGCTACCTGAGTTTAAGATGCTGCGGTTAGGA AATGCAGATAGTTTCCATTACACGAAGCAAGGAGGCAGCCCTATGATAGAAGGAGTGGACGATGCGAAGG AGATGGCACACACCAGGCAGGCCTGCACTCTGCTGGGAATTAGTGAATCTTACCAAATGGGAATTTTTCG GATACTTGCTGGCATTCTTCACTTAGGCAATGTTGGGTTTGCGTCTCGAGATTCAGACAGCTGCACAATA CCTCCCAAGCACGAGCCTCTTATCATTTTCTGTGATCTTATGGGTGTGGATTATGAAGAGATGTGTCACT GGCTCTGCCACCGAAAGCTAGCTACCGCCACAGAGACGTACATCAAGCCCATCTCCAAGCTGCAGGCCAC AAATGCCCGAGACGCTTTAGCAAAGCATATCTATGCAAAGCTCTTTAACTGGATTGTCGGCCATGTCAAT CAGGCTCTCCATTCAGCTGTCAAGCAGCACTCTTTCATCGGCGTGCTGGACATTTATGGATTTGAAACAT TTGAGATAAATAGTTTTGAACAGTTCTGCATAAATTATGCAAATGAAAAGCTACAGCAACAATTCAACAT GCATGTCTTCAAACTGGAACAGGAGGAGTACATGAAGGAACAAATTCCATGGACACTCATAGATTTCTAT GATAATCAGCCTTGTATCAATCTTATAGAATCTAAACTGGGTATTCTAGATTTGCTGGATGAGGAATGTA AGATGCCTAAAGGTACCGATGACACATGGGCCCAAAAACTATACAACACACATTTGAACAAATGTGCTCT CTTTGAGAAGCCCCGCATGTCAAACAAAGCTTTCATCATCAAACATTTTGCTGACAAAGTGGAGTACCAG TGTGAAGGTTTTCTTGAAAAGAATAAAGACACTGTTTTTGAAGAACAAATTAAAGTCCTTAAGTCAAGCA AGTTTAAGATGCTACCAGAACTATTTCAAGATGACGAGAAGGCCATCAGTCCCACCTCTGCCACTTCCTC AGGACGCACACCTCTCACACGGGTACCTGTAAAGCCCACCAAGGGTCGACCTGGCCAGACTGCCAAAGAG CACAAGAAGACAGTGGGACTTCAGTTTCGAAACTCCCTTCACCTGCTTATGGAAACCCTTAACGCCACTA CTCCTCACTATGTTCGCTGTATTAAGCCCAATGATTTCAAGTTTCCATTCACATTTGATGAGAAGAGAGC AGTACAGCAGCTAAGAGCATGTGGTGTCCTGGAGACCATCCGGATCAGCGCAGCAGGGTTTCCCTCAAGG TGGACTTATCAAGAGTTTTTCAGCCGGTACCGGGTCCTAATGAAGCAAAAGGATGTGCTGGGAGATAGAA AGCAAACATGCCAGAATGTATTAGAGAAACTGATACTGGACAAGGATAAATACCAGTTTGGTAAGACAAA GATCTTTTTTCGTGCTGGTCAAGTGGCCTATCTAGAAAAATTGAGGGCTGACAAACTGAGGGCGGCCTGC ATCCGGATCCAGAAGACGATTCGTGGGTGGCTGCTGAGGAAGAGATACCTGTGTATGCAGAGGGCAGCCA TCACAGTGCAGCGATACGTGCGTGGCTATCAGGCTCGATGCTATGCTAAGTTTCTGCGCAGAACCAAGGC AGCAACCACCATTCAGAAGTACTGGCGCATGTATGTGGTCCGCAGGAAGTACAAGATTAGACGAGCTGCC ACCATTGTTCTTCAGTCTTATTTGAGAGGCTACTTGGCAAGAAATAGGTATCGCAAGATACTCCGTGAGC ACAAAGCAGTCATCATTCAGAAACGTGTCCGTGGCTGGCTGGCTCGTACACATTATAAGAGGACCATGAA AGCCATCATCTACCTTCAATGCTGCTTCCGGCGGATGATGGCCAAGCGTGAGCTGAAGAAACTCAAAATT GAGGCTCGCTCTGTGGAACGCTACAAGAAGCTCCATATTGGCATGGAAAACAAGATTATGCAGCTGCAAC GCAAAGTGGATGAGCAGAATAAAGACTACAAATGCCTCATGGAGAAACTGACCAATCTAGAAGGAGTATA CAACTCTGAGACTGAAAAACTACGAAATGATGTAGAACGTCTTCAGCTAAGCGAGGAGGAAGCTAAGGTT GCCACTGGGAGAGTGCTTAGTCTGCAGGAAGAAATTGCAAAACTCCGAAAAGACCTGGAGCAAACTCGAT CGGAGAAAAAGTCCATTGAAGAACGAGCAGATAAATACAAACAAGAAACTGAGCAGCTGGTGTCAAACTT GAAGGAAGAAAACACTCTGCTGAAGCAGGAAAAGGAGACCCTCAATCACCTCATGGTGGAGCAGGCTAAG GAGATGACAGAAACTATGGAGAGGAAGTTAGTAGAAGAAACAAAACAACTGGAGCTCGACCTGAATGATG AGAGGCTGAGGTATCAAAACCTCCTGAATGAGTTCAGTCGTCTGGAGGAGCGCTATGACGACCTCAAGGA AGAGATGACCCTGATGCTGAATGTGCCTAAGCCAGGACACAAGAGAACAGACTCTACCCACAGCAGCAAT GAGTCTGAATACACCTTCAGCTCAGAATTTGCAGAAACTGAAGACATTGCACCAAGGACAGAGGAGCCAA CTGAGAAGAAGGTGCCTTTGGATATGTCATTGTTCCTTAAGCTCCAGAAGCGTGTTACAGAGCTGGGACA GGAGAAGCAGTTGATGCAGGATGAGCTGGACCGAAAGGAGGAGCAGGTGTTGCGCAGCAAGGCAAAGGGA GGAGAAAGACCACAGATTAGAGGTGCTGAACTGGGATATGAGTCCCTCAAGCGTCAGGAACTGGAGTCAG AAAACAAAAAGCTGAAGAATGAGCTGAATGAGCTACGCAAAGCCCTGAGTGAGAAGAGTGCCCCCGAAGT GAATGCGCCAGGCGCGCCCGCTTACCGCGTCCTCATGGAGCAGCTGACGGCCGTGAGCGAGGAGCTCGAT GTCCGCAAGGAGGAAGTCCTCATTTTAAGGTCTCAGCTGGTGAGCCAGAAAGAAGCCATCCAACCCAAGG ATGACAAGAATACAATGACAGATTCCACAATACTTTTGGAAGATGTACAGAAAATGAAAGACAAAGGTGA AATAGCACAAGCATATATTGGTTTGAAAGAAACGAACAGGCTCTTAGAATCCCAGCTACAGTCGCAGAAG AGGAGCCATGAAAATGAGGCTGAGGCCCTCCGTGGGGAGATCCAGAGCCTAAAGGAAGAAAACAACCGGC AACAGCAGCTGCTGGCCCAGAACCTGCAGCTGCCCCCTGAGGCCCGCATTGAGGCCAGCCTGCAGCATGA GATCACCCGGCTGACCAATGAAAACCTGGATCTGATGGAACAACTTGAAAAGCAGGATAAAACCGTCCGG AAACTGAAGAAACAACTAAAAGTCTTTGCCAAAAAAATCGGTGAACTAGAAGTGGGGCAGATGGAGAACA TATCCCCAGGACAGATCATCGATGAGCCTATCCGGCCGGTCAACATTCCCCGGAAAGGAAAGGATTTCCA AGGGATGCTGGAGTACAAGCGGGAGGACGAACAGAAGCTTGTTAAGAACCTGATTCTAGAACTAAAGCCA CGTGGTGTGGCTGTCAATCTGATTTCAGGGTTACCGGCATATATCTTGTTTATGTGTGTGCGACATGCCG ACTACCTGGACGATGATCAGAAAGTAAGATCATTGCTGACATCAACAATTAACAGCATCAAAAAAGTCCT GAAGAAAAGAGGTGACGATTTTGAAACTGTCTCCTTCTGGCTCTCTAACACATGTCGATTTTTGCACTGT TTGAAGCAATATAGTGGAGAAGAGGGCTTCATGAAACACAACACGTCTCGCCAGAATGAACACTGCCTCA CCAATTTTGACCTTGCTGAGTATCGGCAAGTACTGAGTGACTTGGCCATTCAGATCTATCAGCAGCTTGT GAGGGTGTTAGAGAACATTCTTCAGCCAATGATAGTCTCAGGCATGCTAGAGCATGAAACAATTCAGGGA GTATCTGGGGTGAAGCCCACAGGACTCAGAAAGCGAACCTCCAGTATCGCCGATGAGGGCACCTACACAC TGGACTCCATCCTGCGGCAGCTCAACTCCTTCCATTCCGTCATGTGTCAGCATGGCATGGACCCAGAGCT AATCAAGCAGGTGGTCAAGCAGATGTTCTACATCGTGGGCGCCATCACCCTGAACAACCTCCTGCTGCGC AAGGACATGTGCTCCTGGAGCAAAGGCATGCAGATCAGGTACAATGTCAGTCAATTGGAAGAATGGCTAC GTGACAAGAATCTAATGAACAGTGGGGCAAAAGAGACTCTGGAACCTCTTATCCAGGCTGCTCAGCTTTT GCAAGTGAAAAAGAAAACTGACGATGACGCGGAAGCCATCTGCTCCATGTGCAACGCTCTGACCACAGCC CAGATTGTCAAAGTGTTGAATCTGTACACACCCGTTAATGAGTTTGAGGAAAGGGTCTCTGTTTCATTTA TCCGCACTATACAGGTGCGGTTACGAGACAGGAAAGACTCTCCACAGCTGCTCATGGATGCAAAACACAT CTTTCCTGTCACTTTCCCCTTTAACCCATCCTCCCTGGCCCTAGAAACCATCCAGATTCCAGCCAGCCTG GGCCTGGGCTTCATTGCAAGGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212159 representing NM_022178 Red=Cloning site Green=Tags(s) MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTSELPHLRNPDILVG ENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIF AVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAK TTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLG NADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTI PPKHEPLIIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVGHVN QALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFY DNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQ CEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKE HKKTVGLQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSR WTYQEFFSRYRVLMKQKDVLGDRKQTCQNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAAC IRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRKYKIRRAA TIVLQSYLRGYLARNRYRKILREHKAVIIQKRVRGWLARTHYKRTMKAIIYLQCCFRRMMAKRELKKLKI EARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKV ATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETEQLVSNLKEENTLLKQEKETLNHLMVEQAK EMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSN ESEYTFSSEFAETEDIAPRTEEPTEKKVPLDMSLFLKLQKRVTELGQEKQLMQDELDRKEEQVLRSKAKG GERPQIRGAELGYESLKRQELESENKKLKNELNELRKALSEKSAPEVNAPGAPAYRVLMEQLTAVSEELD VRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQK RSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVR KLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKGKDFQGMLEYKREDEQKLVKNLILELKP RGVAVNLISGLPAYILFMCVRHADYLDDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHC LKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQG VSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLR KDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTA QIVKVLNLYTPVNEFEERVSVSFIRTIQVRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASL GLGFIARV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022178
ORF Size	5484 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022178.1, NP_071514.1
RefSeq Size	5620 bp
RefSeq ORF	5487 bp
Locus ID	25017
UniProt ID	Q9QYF3
MW	211.8 kDa
Gene Summary	may play a role in secretory granule transport [RGD, Feb 2006]

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