Adgrl1 (NM_022962) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CL1BA; Lphn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211905 representing NM_022962 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGCTTGGCTGCAGCACTCTGGAGTCTCTGTGTGACGACTGTCCTCGTCACCTCTGCTACCCAAG GCCTGAGCCGGGCTGGACTCCCATTTGGATTGATGCGCCGGGAGCTAGCATGCGAAGGCTACCCCATTGA GCTGCGGTGCCCGGGCAGTGACGTCATCATGGTGGAGAATGCAAACTATGGGCGCACAGATGACAAGATC TGCGATGCCGACCCTTTTCAGATGGAGAACGTGCAGTGCTACCTGCCTGACGCCTTCAAAATCATGTCAC AGAGATGTAATAACCGAACCCAGTGTGTGGTGGTGGCCGGCTCTGACGCCTTTCCTGACCCCTGTCCTGG AACCTACAAGTACCTGGAGGTGCAGTACGACTGTGTCCCTTACAAAGTGGAGCAGAAAGTCTTCGTGTGC CCAGGGACACTGCAGAAGGTGCTGGAGCCCACCTCCACACATGAATCGGAGCACCAGTCTGGCGCATGGT GCAAGGACCCACTGCAGGCAGGTGACCGTATCTACGTTATGCCCTGGATCCCCTACCGCACGGACACACT GACCGAGTATGCTTCCTGGGAGGACTATGTGGCTGCACGCCACACCACCACGTACAGACTGCCCAACCGT GTAGATGGCACTGGCTTTGTGGTATATGATGGTGCCGTCTTCTATAACAAGGAACGTACTCGCAACATTG TCAAATATGACCTGCGGACCCGCATCAAGAGCGGAGAAACAGTCATAAACACAGCCAACTACCACGACAC CTCACCTTATCGCTGGGGAGGCAAAACCGACATTGACCTGGCAGTGGATGAGAACGGGCTGTGGGTCATC TATGCCACCGAGGGGAACAACGGGCGTCTGGTGGTGAGCCAGCTCAACCCCTACACACTGCGTTTCGAGG GCACCTGGGAAACAGGCTATGACAAGCGCTCAGCCTCCAATGCCTTCATGGTGTGTGGTGTCCTCTATGT GCTGCGCTCTGTTTATGTGGATGACGACAGTGAGGCAGCAGGCAACCGCGTGGACTATGCCTTTAACACC AATGCAAACCGAGAGGAGCCCGTCAGTCTCGCCTTCCCCAACCCCTACCAGTTTGTATCTTCTGTTGACT ACAATCCCCGGGACAACCAGCTGTATGTGTGGAACAACTATTTCGTGGTGCGCTACAGCCTGGAGTTTGG ACCCCCAGATCCCAGTGCTGGCCCAGCCACTTCCCCACCTCTCAGTACCACCACCACAGCTCGGCCTACG CCCCTCACCAGCACAGCCTCACCTGCAGCCACCACTCCACTCCGCCGGGCGCCCCTCACCACGCACCCAG TAGGTGCCATCAACCAGCTGGGACCTGACCTGCCTCCAGCCACAGCCCCAGCACCCAGTACCCGGCGGCC TCCAGCCCCCAATCTGCATGTGTCCCCTGAGCTCTTCTGTGAACCCCGAGAGGTCCGGCGGGTCCAGTGG CCAGCTACCCAGCAGGGTATGCTGGTAGAGAGACCTTGCCCCAAGGGAACTCGAGGAATTGCCTCGTTCC AGTGCCTCCCAGCTCTGGGGCTCTGGAATCCTCGGGGCCCTGACCTCAGCAACTGCACTTCCCCCTGGGT CAACCAAGTCGCCCAGAAGATCAAGAGTGGAGAGAATGCAGCCAACATTGCTAGTGAGCTGGCCCGCCAC ACGCGGGGCTCCATCTATGCTGGGGACGTGTCCTCATCGGTGAAGCTGATGGAGCAACTGCTAGATATCC TGGATGCCCAGCTCCAGGCCCTACGGCCCATTGAACGAGAGTCAGCTGGCAAGAACTACAATAAGATGCA CAAGCGAGAGAGAACCTGCAAGGACTATATCAAGGCTGTGGTGGAGACAGTGGACAACCTGCTTCGGCCA GAGGCACTTGAGTCATGGAAAGACATGAATGCCACCGAACAGGTCCATACGGCCACCATGCTCCTAGATG TCTTAGAGGAGGGTGCCTTCCTGCTGGCCGACAATGTCAGAGAACCTGCTCGCTTCTTGGCTGCCAAGCA GAATGTGGTCCTGGAGGTCACTGTCCTGAGCACAGAGGGTCAAGTGCAGGAGTTGGTGTTCCCCCAGGAG TATGCCAGTGAGAGCTCCATTCAGCTGTCCGCCAACACCATCAAGCAGAACAGCCGCAATGGTGTGGTGA AGGTTGTCTTCATTCTCTACAACAACCTGGGCCTCTTCTTGTCCACGGAGAATGCCACAGTGAAGCTGGC AGGTGAGGCAGGGACCGGTGGCCCTGGAGGTGCCTCCCTGGTGGTTAACTCACAGGTCATCGCAGCATCC ATCAATAAGGAGTCCAGCCGTGTCTTCCTCATGGACCCTGTCATCTTTACTGTGGCCCACTTGGAGGCCA AGAACCACTTCAATGCAAACTGCTCCTTCTGGAACTACTCAGAGCGCTCCATGCTGGGCTACTGGTCAAC CCAGGGCTGCCGACTGGTGGAGTCCAATAAGACCCATACCACATGTGCCTGCAGCCACCTCACCAACTTC GCAGTGCTCATGGCTCACCGAGAGATCTACCAAGGCCGTATTAATGAGCTGTTGCTGTCAGTCATCACCT GGGTTGGCATTGTCATCTCCCTGGTCTGTCTGGCTATCTGCATCTCCACCTTCTGCTTCCTGCGGGGCCT GCAGACCGACCGCAACACCATCCACAAGAACCTGTGCATCAACCTCTTCCTTGCAGAGCTGCTCTTCCTG GTTGGAATAGACAAAACTCAGTATGAGGTCGCCTGCCCTATCTTTGCGGGCCTGCTGCACTACTTCTTCC TGGCCGCCTTCTCCTGGCTGTGCCTAGAGGGCGTGCACCTCTACCTCCTGCTGGTCGAGGTGTTCGAGAG CGAATATTCACGCACCAAGTACTATTACCTGGGCGGCTACTGCTTCCCAGCCCTGGTGGTAGGCATCGCA GCCGCCATTGACTACCGAAGCTACGGCACTGAGAAGGCCTGCTGGCTGAGGGTGGATAACTATTTCATCT GGAGCTTCATTGGGCCCGTCTCCTTTGTTATTGTGGTGAACCTGGTGTTCCTCATGGTGACCCTGCACAA GATGATCCGAAGCTCATCCGTGCTCAAGCCTGACTCCAGCCGCCTTGACAACATCAAGTCCTGGGCGCTG GGTGCCATTGCACTGCTCTTCCTGCTGGGCCTCACCTGGGCTTTCGGCCTCCTCTTCATCAACAAGGAGT CAGTAGTAATGGCTTACCTCTTCACAACCTTCAACGCCTTCCAGGGGGTCTTCATCTTTGTCTTTCACTG CGCCTTACAGAAAAAGGTGCACAAGGAGTACAGCAAGTGCCTGCGTCACTCCTACTGCTGCATTCGCTCC CCACCTGGGGGGGCTCACGGCTCCCTTAAGACCTCAGCCATGCGAAGTAACACCCGCTACTACACAGGGA CCCAGAGCCGAATCCGGAGGATGTGGAATGACACCGTGAGGAAGCAGACAGAGTCGTCCTTTATGGCAGG GGACATCAACAGCACCCCCACCCTGAACCGAGGTACCATGGGGAACCACCTACTGACCAACCCTGTGCTA CAGCCCCGTGGGGGCACTAGCCCATACAATACACTCATTGCAGAGTCTGTGGGCTTCAATCCCTCCTCGC CCCCAGTCTTCAACTCCCCAGGAAGCTACAGGGAACCTAAGCACCCCTTGGGCGGCCGGGAAGCCTGTGG CATGGACACACTGCCCCTTAATGGCAACTTCAACAACAGCTACTCCTTGCGAAGTGGTGATTTCCCTCCG GGGGATGGGGGTCCTGAGCCACCCCGAGGCCGAAACCTAGCGGATGCTGCGGCCTTTGAGAAGATGATCA TCTCAGAGCTGGTGCACAACAACCTTCGGGGGGCCAGTGGGGGCGCCAAAGGTCCTCCACCAGAGCCTCC TGTGCCACCCGTGCCAGGAGTCAGTGAGGACGAGGCTGGTGGGCCTGGGGGTGCTGACCGGGCTGAGATT GAACTTCTCTACAAGGCCCTGGAGGAGCCACTGCTGCTGCCCCGGGCCCAGTCGGTGCTGTACCAGAGTG ATCTGGATGAGTCGGAGAGCTGTACGGCAGAGGATGGGGCCACCAGCCGGCCCCTCTCCTCCCCTCCCGG CCGGGACTCCCTCTATGCCAGCGGGGCCAACCTGCGGGACTCGCCCTCCTACCCGGACAGCAGCCCCGAA GGGCCTAATGAGGCCCTGCCCCCTCCCCCACCTGCTCCCCCTGGGCCCCCAGAAATCTACTACACCTCTC GCCCGCCGGCCCTGGTGGCTCGGAATCCCCTACAGGGCTACTACCAGGTGCGGCGGCCCAGCCATGAGGG CTACCTGGCAGCCCCCAGCCTTGAGGGGCCAGGGCCCGATGGGGATGGGCAAATGCAGTTGGTCACTAGT CTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211905 representing NM_022962 Red=Cloning site Green=Tags(s) MARLAAALWSLCVTTVLVTSATQGLSRAGLPFGLMRRELACEGYPIELRCPGSDVIMVENANYGRTDDKI CDADPFQMENVQCYLPDAFKIMSQRCNNRTQCVVVAGSDAFPDPCPGTYKYLEVQYDCVPYKVEQKVFVC PGTLQKVLEPTSTHESEHQSGAWCKDPLQAGDRIYVMPWIPYRTDTLTEYASWEDYVAARHTTTYRLPNR VDGTGFVVYDGAVFYNKERTRNIVKYDLRTRIKSGETVINTANYHDTSPYRWGGKTDIDLAVDENGLWVI YATEGNNGRLVVSQLNPYTLRFEGTWETGYDKRSASNAFMVCGVLYVLRSVYVDDDSEAAGNRVDYAFNT NANREEPVSLAFPNPYQFVSSVDYNPRDNQLYVWNNYFVVRYSLEFGPPDPSAGPATSPPLSTTTTARPT PLTSTASPAATTPLRRAPLTTHPVGAINQLGPDLPPATAPAPSTRRPPAPNLHVSPELFCEPREVRRVQW PATQQGMLVERPCPKGTRGIASFQCLPALGLWNPRGPDLSNCTSPWVNQVAQKIKSGENAANIASELARH TRGSIYAGDVSSSVKLMEQLLDILDAQLQALRPIERESAGKNYNKMHKRERTCKDYIKAVVETVDNLLRP EALESWKDMNATEQVHTATMLLDVLEEGAFLLADNVREPARFLAAKQNVVLEVTVLSTEGQVQELVFPQE YASESSIQLSANTIKQNSRNGVVKVVFILYNNLGLFLSTENATVKLAGEAGTGGPGGASLVVNSQVIAAS INKESSRVFLMDPVIFTVAHLEAKNHFNANCSFWNYSERSMLGYWSTQGCRLVESNKTHTTCACSHLTNF AVLMAHREIYQGRINELLLSVITWVGIVISLVCLAICISTFCFLRGLQTDRNTIHKNLCINLFLAELLFL VGIDKTQYEVACPIFAGLLHYFFLAAFSWLCLEGVHLYLLLVEVFESEYSRTKYYYLGGYCFPALVVGIA AAIDYRSYGTEKACWLRVDNYFIWSFIGPVSFVIVVNLVFLMVTLHKMIRSSSVLKPDSSRLDNIKSWAL GAIALLFLLGLTWAFGLLFINKESVVMAYLFTTFNAFQGVFIFVFHCALQKKVHKEYSKCLRHSYCCIRS PPGGAHGSLKTSAMRSNTRYYTGTQSRIRRMWNDTVRKQTESSFMAGDINSTPTLNRGTMGNHLLTNPVL QPRGGTSPYNTLIAESVGFNPSSPPVFNSPGSYREPKHPLGGREACGMDTLPLNGNFNNSYSLRSGDFPP GDGGPEPPRGRNLADAAAFEKMIISELVHNNLRGASGGAKGPPPEPPVPPVPGVSEDEAGGPGGADRAEI ELLYKALEEPLLLPRAQSVLYQSDLDESESCTAEDGATSRPLSSPPGRDSLYASGANLRDSPSYPDSSPE GPNEALPPPPPAPPGPPEIYYTSRPPALVARNPLQGYYQVRRPSHEGYLAAPSLEGPGPDGDGQMQLVTS L TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022962
ORF Size	4413 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022962.1, NP_075251.1
RefSeq Size	5579 bp
RefSeq ORF	4416 bp
Locus ID	65096
UniProt ID	O88917
MW	162.1 kDa
Gene Summary	G protein-coupled receptor for alpha-Latrotoxin [RGD, Feb 2006]

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