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Epas1 (NM_023090) Rat Tagged ORF Clone
CAT＃: RR210531
- TrueORF®
Epas1 (Myc-DDK-tagged ORF) - Rat endothelial PAS domain protein 1 (Epas1), (10 ug)
"NM_023090" in other vectors (3)
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CNY 11,210.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HIF-2 alpha; HIF2 alpha; Hif2a; HLF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210531 representing NM_023090
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCTGACAAGGAGAAGAAAAGGAGCAGCTCGGAGCTGAGGAAGGAGAAATCCCGAGATGCCGCAC GGTGCCGGCGTAGCAAGGAGACGGAGGTCTTCTATGAACTGGCTCATGAGCTGCCGCTGCCCCACAGTGT GAGCTCCCACCTGGACAAAGCCTCCATCATGCGCCTGGCCATCAGCTTCCTGCGAACACATAAACTCCTG TCTTCAGTGTGCTCTGAAAATGAATCTGAAGCCGAGGCTGACCAGCAGATGGATAACTTGTACCTGAAAG CCTTGGAGGGCTTCATTGCCGTGGTGACCCAAGACGGTGATATGATCTTTCTGTCAGAAAACATCAGCAA GTTCATGGGACTTACCCAGGTAGAACTAACAGGACACAGCATCTTTGACTTCACTCATCCTTGCGACCAC GAGGAGATCCGAGAGAACCTGACACTCAAAACTGGCTCTGGTTTTGGGAAGAAGAACAAAGATAGGTCTA CCGAGCGTGACTTCTTCATGAGGATGAAGTGTACCGTCACCAACAGAGGCCGGACTGTCAACCTTAAGTC GGCCACCTGGAAGGTCTTGCACTGCACCGGGCAAGTGAGAGTCTACAACAACTGCCCCCCTCACAGTAGC CTCTGCGGCTACAAGGAGCCCCTGCTGTCCTGCCTCATCATCATGTGTGAGCCAATCCAGCACCCATCCC ACATGGACATCCCCTTGGACAGCAAGACTTTCCTAAGCCGCCACAGCATGGACATGAAGTTCACCTACTG TGATGACAGAATCTTGGAACTGGTTGGTTACCACCCCGAGGAGCTACTTGGCCGCTCTGCCTATGAATTC TACCACGCCCTGGACTCGGAGAACATGACAAAAAGTCACCAGAACTTGTGCACCAAGGGGCAAGTGGTGT CTGGCCAGTACCGGATGCTGGCCAAACATGGAGGATATGTGTGGTTGGAGACCCAGGGGACCGTCGTCTA CAACCCCCGCAACCTGCAGCCCCAGTGTATCATGTGTGTCAACTATGTCCTGAGTGAGATAGAGAAGAAT GACGTGGTGGTCTCCATGGACCAGACTGAATCTTGTTCAAGCCACACCCTGATGGCCATGAACAGCATCT TTGACACAAGTGACGACGTGGCCCTATCCGAGAAGAGCAACTACCTGTTCACCAACCTGAAGGAGGAGCC AGAGGACCTGGCCCAGCTGGCCCCCACCCCAGGGGATGCTATTATTTCTCTGGACTTCGGAAGCCAGAAC TTTGATGAATCCTCGACTTATGGCAAGGCCATCCTTCCCCCAGGCCAGCCGTGGGCCACAGAGCTGAGGA GCCACAGTGCCCAGAGTGAGTCCAGGAGCCTGCCAGCCTTCACCGTGCCCCAGGCAGGTTCCCCAGGGAA TGCCACACCCAGCGCCACAAGCAGCAGCAGCTGCTCCACGCCCAGCAGCCCTGAGGATTACTACTCATCT CTGGAGAATCACTTGAAGATCGAAGTAATCGAGAAGCTCTTCGCCATGGACACCGAGGCGAAGGATCAGT GCAGTACTCAGACGGACTTCAATGAACTGGACTTGGAGACCCTGGCACCCTACATCCCTATGGATGGCGA GGACTTCCAGCTCAGCCCCATCTGCCCGGAGGAGCCACTCGTGCCGGAGAGCCCCCAGCCCAACCCCCAG CACTGTTTCAGTACCATGTCAAGCATCTTCCAGCCGCTCACCCCGGGGGCCAGCCAAGGCACCTTCTTCC TTGACAAGTACCCGCAGCAGTTGGAAAGCCGGAAGACGGAGTCCGAGCACTGGCCCATGTCTACCATCTT CTTTGATGCTGGGAGCAAAGGGTCCCTGCCCCCATGCTGTGGCCAGGCCAGCACCCCTCTCTCCTCTATG GGGGGCAGATCTAACACGCCTTGGCCCCCAGATCCACCATTACATTTAGGCCCTACGAAGTGGTCTGTGG GCAATCAGAGCGCTGAACCCCTGGGGCCTCTGCCACTGGGGACCTCGCAGTTGGAGCCTCCCAGCACCCC GCCTCATGTCTCCATGTTCAAGATGAGGTCTGCAAAGGACTTTGGGGCTCGAGGCCCATACATGATGAGC CCGGCCATGATAGCCCTGTCCAACAAGCTGAAGCTAAAGCGCCAGCTGGACTACGAGGAGCCAGCCTTCC AAGACACAAGTGGGGGGGACCCTCCAGGCACCAGCAGTTCACACTTGATGTGGAAACGAATGAAGAGCCT CATGGGCGGGACCTGTCCTTTGATGCCTGACAAGACCGTCAGTGCAAGCATGGCTCCTGATGAATTCACC CAAAAATCCATGAGAGGCCTGGGCCAGCCACTGAGACACCTGCCACCTTCCCAGCCACCATCTACCAGGA GTCCAGGAGAGAACGCCAAGAGTGGGTTCCCACCACAGTGCTATGCCTCCCCGTTCCAGGACTACAGTCC TCCAGGAGCTCAAAAGGGGTCAGGAGTGGCAAGTCGACTGCTGGGGCCATCGTTCGAGCCTTACCTGCTG CCGGAACTGACCAGATATGACTGTGAGGTGAACGTGCCCGAGCCTGGAAGTTCTACACTCCTGCAGGGGA GAGACCTTCTCAGAGCTCTGGACCAGGCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210531 representing NM_023090
Red=Cloning site Green=Tags(s) MTADKEKKRSSSELRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLL SSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDH EEIRENLTLKTGSGFGKKNKDRSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVRVYNNCPPHSS LCGYKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRILELVGYHPEELLGRSAYEF YHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVVYNPRNLQPQCIMCVNYVLSEIEKN DVVVSMDQTESCSSHTLMAMNSIFDTSDDVALSEKSNYLFTNLKEEPEDLAQLAPTPGDAIISLDFGSQN FDESSTYGKAILPPGQPWATELRSHSAQSESRSLPAFTVPQAGSPGNATPSATSSSSCSTPSSPEDYYSS LENHLKIEVIEKLFAMDTEAKDQCSTQTDFNELDLETLAPYIPMDGEDFQLSPICPEEPLVPESPQPNPQ HCFSTMSSIFQPLTPGASQGTFFLDKYPQQLESRKTESEHWPMSTIFFDAGSKGSLPPCCGQASTPLSSM GGRSNTPWPPDPPLHLGPTKWSVGNQSAEPLGPLPLGTSQLEPPSTPPHVSMFKMRSAKDFGARGPYMMS PAMIALSNKLKLKRQLDYEEPAFQDTSGGDPPGTSSSHLMWKRMKSLMGGTCPLMPDKTVSASMAPDEFT QKSMRGLGQPLRHLPPSQPPSTRSPGENAKSGFPPQCYASPFQDYSPPGAQKGSGVASRLLGPSFEPYLL PELTRYDCEVNVPEPGSSTLLQGRDLLRALDQAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023090 |
| ORF Size | 2622 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023090.1, NP_075578.1 |
| RefSeq Size | 2730 bp |
| RefSeq ORF | 2625 bp |
| Locus ID | 29452 |
| UniProt ID | Q9JHS1 |
| MW | 96.7 kDa |
| Gene Summary | activates luciferase activity after transfection [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210531 | Epas1 (untagged ORF) - Rat endothelial PAS domain protein 1 (Epas1), (10 ug) |
CNY 11,210.00 |
|
| RR210531L3 | Lenti ORF clone of Epas1 (Myc-DDK-tagged ORF) - Rat endothelial PAS domain protein 1 (Epas1), (10 ug) |
CNY 13,020.00 |
|
| RR210531L4 | Lenti ORF clone of Epas1 (mGFP-tagged ORF) - Rat endothelial PAS domain protein 1 (Epas1), (10 ug) |
CNY 14,160.00 |
