Setd2 (NM_001108189) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Kif9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210372 representing NM_001108189 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAGTGTACCAGAATCTTTAGAGTTGGATATGAAGCAAGATATTGTATCTAACAGTTTGGAAGAAC ACACAAATCAAACTTTGAAGGAACAAGCAGATAATGCCCTGCAAAAAGAAGATTCCCATATTGGGAAGGA AGAAGAGGTTTCAGATGGCTCTAAGATAAGCCTCAGTTCTAAAAAAGCAAGTTCTAAGAAGAAATCTTCA CAATTTGAAGGTACATTTCTTGGTTCAGAATCTGATGAAGATTCTGTACGGACTTCCTCCAGTCAAAGAT CACATGATTTAAAATCTTCAACAAGCATTGAGAAAGAAAGAGATTTTAAGAAGAGCTCAGCACCTTCAAA AAGTGAGGATTTGGGAAAATCATCAAGATCTAAAACAGAGAGAGATGATAAATACTTTAGCTACTCAAAA CTTGAAAGAGACACTCGGTATGTATCTACTCGATGTAGGTCTGAAAGAGATCGAAGGCGAAGCCGATCTC GTTCTAGATCTGACAGAGTCTCTAGAACTAGTTTGTCTTATTCTCGGTCAGAAAGATCTCATTATTATGA TTCTGAACGCCGTTACCATAGGAGTTCCCCTTATCGTGAGAGGACACGCTATTCTCGGCCATATACAGAT AACAGGGCACGGGAGAGCTCAGACTCTGAAGACGAGTATAAGAAGACATATCCAAGGCGTACCTCAGCCC ACTCCTACAGAGACCTAAGGACATCATCATCTTACTCTAAATTTGATCGGGACTGTAAAACTGAGACCTC TTACTTAGAAATGGAGAGAAGAGGAAAGTATTCTTCAAAACTAGAAAGAGAATCCAAACGGACTTCAGAG CATGAAGCCATAAAAAGATGTTGTTCTCCCCCAAATGAATTGGGATTCCGACGGGGATCATCATATTCCA AGCATGATAACAACAGTACTTCCCGTTATAAATCTGCCCTTTCAAAATCTATATCTAAGTCTGATAAATT TAAAAATTCTTTCTGTTGTACAGAATTAAATGAGGAAAACAAACAATCTCATTCTTTTAGTTTGCAGACG CCTTGTTCAAAAGGTAGTGAATTAAGAACAATTAATAAAATTCCTGAAAGAGAAAAGACTGGGTCTCCAT CTCCATCAAATCAATTAAATGATTCACCTACTTTTAAAAAGCTAGATGAATCTCCTATTCTTAAGCCTGA ATTTATAGGACATGATAGCCATGAGAGTATTAAAGAATTGGAGTTACCAAAAATGAAAAATGATCAATTG AGAAATTTTTGTTCCATTGAATTAAATGTAAACGGATCTCCAGAGACAGAAACTGATGTGGCAACATTTC GAACTTCTAAACCAGATGCTATCTCAATGACTTCTGATGATAGTGTGACTGGATCAGAGGTATCCCCTTT GATCAAGGGTTGCGTGCTTTCATCAAATGGATTTCAGAGTGTTGGTAGATGCAGAGAAAGGGATGCAGAT GATGCTTGTAGGCAGCATAATAAATCAAAAAGTCCATTTAGGGAAACGGAACCTTTGTTGTCACCACACC ATGATAAAGTCATGTCTTTGCCAGTTAAGACTATAGATTATTCCAAAACGTTAATTAAAGAGCCAGTTGA TAAGAGACATTCTTGCTGCAAAACCAAAGATTCAGATAGATACTGTTCTCCAAATGAAAGCTCTGAAGCT GAAAATAGGGAGATTTCTTCAAATTGCTTTGTGAATGTGCATTTGGATTCCAAAACAGTTGTCTGTGATA ATAGGGAGCTGACAGACCAGCACTCAGAATTTACGTGTGACGGATATAAGCAGAGCATTGGTAGCACTAG CTCGGCCTCTCTTAACCATTTTGATGACTTGTATGAGTCTGTAGGGAGTTCAGGTATTTCATCTCTCCAG AGTCTTCCATCAGGAATAAGATGTGAAGAGAACACGTCTCCAGCTCTAGACACAGTGCAGAGTAAAAAAG GTATAGATTTTTTAAAGTATGTAGGGAAAGAGACAGATGTTGTTAGTGCCCTTCCCGATTCAGGAAAAGC ATTTTCTTCTTGGGAAAACAGGCATAATATGTTGTCTGGGCAGTCTTTGCAAGAGTCTCAAGAAGAAGGG AGTTCCACATTGCATGAGAGAAGAGGAAGATCAGAAGTCTCCTTAGATGAGGAAGAACAAAGGGGTCATA CACATATTTCTGATGATTCGGAAGTTGTATTTCCTTATGATTTGAACTTAACCATGGAAGACAGTGATGG TGTAACTTACACCTTAAAATGTGACAGCAGTGGTAATGCTCCCGAGATTGTATCAACTGTCCATGGAGAT TACTCTGCGTCTTCTGCAAGTTCAAGCGATGAGAGTGATTCAGAAGATACAGAGTCTGATGACAGCAGTA TTCCGAGAAACCGTCTCCAGTCTGTTGTGGTTGTGCCAAAGAATTCTACTTTGCCCATGGAAGAGACAAG TCCCTGCTCTTCTCGGAGCAGTCAGAGCTACAGACATTATTCCGACCACTGGGAGGATGGGTTAGAGTCG AGGAGACATGCGTATGAGGAAAAGTTTGAGAGTATGGCAAGTAAAGGCTGTTCCCAAACTGAAAAGTTCT TTCTTCATAAAGGAACAGAGAGGAATCCAGAAACATCTTATTCACAATTCAGCAGGAAGATAGATAATCA CCTGCCTGAAATTGCTCATTCTCAGAGTGATGGGGTTGATAGTACAAGTCATACAGACGTGAAGTCTGAC TCTGTAGGTCACCTGAATTCAGAAGACGCAATAAGAGCCAAAATGTCCAGGCCACAAGAGCTCCCAGTCT ATTGTGATGACTTTGAAGACCTCCCAAATACGTCTCGGCAGCAGATGATTTTCTCCAATCGGCCAGATAG TAGTAGACTAGGAAAAGCCGAGCTGAGTTTTTCTTCCTCTTGTGACATTTCCCGAATGGATGGCTTGCAC TCATCAGAAGAGCTCAGAAACCTAGGGTGGGACTTTTCCCAACAAGAAAGGCCCACCACCACATACCAGC AGCCTGACAGCAGCTATGGAACTTGTGGTACACACAAGTATCAACAGAGTGCCGAGCATTACGGTGGGAC CCATGATTACTGGCAAGGCAATGGCTATTGGGATCCAAGGTCAGCAGGTAGACCTCCAGGAACTGGGGTT GTTTATGATCGAATCCAAGGGCAAGTACCAGATTCTCTAACAGATGATCGTGAAGAAGAGGAAAATTGGG ATCAACGAAGTGGATCACATTTTTCAAGCCCATCCAATAAATTTTTCTTCCATCAGAAGGACAAGGGATC AGTGCAAGCACCAGAAATAAGCAGCAATTCAATTAAAGACTCTTTAGTTATAAACGAAAGGAAAGATTTT TCAAAAAACTTCGAAAAAAATGATATAAAAGAGCGAGGACCTCCTAAAAAACGAAGGCAAGAATTGGAGA GTGATTCTGAAAGTGATGGTGAGCTACAGGCTAGAAAGAAAGTTAGAGTGGAGATAGAACAGGGACAGTC GTCTGTGCCCCAGCACTCAGAGCTGGTGGGGCCTTCGTGTGCTATGGATGACTTCAGGGACCCGCAGCGG TGGAAAGAGTTTGCCAAGCTGGGGAAGATGCCTTGTTACTTTGATCTGATTGAAGAAAATGTTTATTTAA CAGAAAGGAAGAAGAACAAATCCCATCGGGATATTAAGCGGATGCAGTGTGAGTGCACACCTCTTTCTAA AGATGAACGAGCTCAAGGTGAAGTTGCCTGTGGAGAGGATTGCCTTAATCGTCTCCTCATGATTGAATGT TCCTCTCGATGTCCAAATGGAGATTATTGTTCGAACAGACGGTTTCAGAGAAAACAGCACGCAGATGTAG AAGTCATACTTACAGAAAAGAAAGGCTGGGGCTTAAGGGCCGCCAAGGATCTTCCTTCGAACACCTTTGT CCTGGAATACTGCGGAGAGGTACTTGATCATAAAGAGTTTAAAGCTCGGGTGAAGGAATATGCGCGGAAC AAAAACATCCACTACTATTTCATGGCCCTGAAGAATGATGAGATAATAGATGCCACTCAGAAAGGGAACT GCTCTCGTTTTATGAATCATAGCTGTGAACCAAACTGTGAAACCCAAAAATGGACTGTGAATGGACAGCT GAGGGTTGGATTTTTTACCACCAAATTAGTTCCTTCAGGCTCAGAATTAACGTTTGATTACCAGTTCCAA CGATATGGCAAGGAGGCTCAGAAGTGTTTCTGTGGATCAGCGAACTGTCGGGGTTACTTGGGAGGAGAAA ACAGAGTCAGTATCAGAGCTGCAGGGGGGAAGATGAAAAAGGAACGCTCTCGAAAGAAGGATTCAGTGGA TGGAGAACTTGAAGCACTGATGGAAAATGGTGAAGGCCTCTCTGATAAGAACCAAGTTCTTAGTTTATCC CGGCTCATGGTTAGAATCGAAACTTTGGAACAGAAGCTTACCTGTCTCAAGCTTATTCAGAACACACACT CACAGTCCTGTCTCAAGTCATTTCTGGAACGGCATGGGTTGTCACTGTTGTGGATCTGGATGGCAGAGCT CGGCGATGGCCGGGAAAGTAACCAGAAGCTTCAGGAAGAGATTATAAAGACTTTGGAGCATTTGCCCATT CCTACTAAAAATATGTTGGAAGAAAGCAAAGTACTTCCAATTATTCAACGCTGGTCTCAGACTAAGACTG CTGTTCCTCAGTTAAGTGAAGGAGACGGGTATTCTAGTGAGAATACATCACGTGCTCACACACCGCTTAA TACACCGGACCCTTCTGCCAAGCCGAGCACAGAGGTGGATACAGATACTCCCAAGAAACTCATATTTCGC AGACTAAAGATTATAAGTGAAAATAGCATGGACAGTGCAGTCTCAGACGTCACCAGTGAGCTAGAATGCA AGGATGGCAAAGAGGACCTGGATCAGTTAGAGACTGTCACTGTGGAGGAAGATGAAGAACTGCAGTCCCA ACAGCTCCTGCCATCACAACTCTGCGAGTCCAAAGTTGAGAGTGAAAGCACCATCGACGTCAGTAAATTA CCCATATCTGAACCGGAGGCTGACACTGAGACCGAGCCCAAAGACAGCAATGGCACAAAACTAGAAGAAA CTATTGCCGAGGAAACACCATCCCAAGATGAGGAAGAGGGTGTGTCTGATGTTGAGAGTGAGAGAAGCCA GGAACCACCAGATAAAACAGTGGATATAAGTGATTTGGCTACCAAGCTACTAGACAGTTGGAAAGATCTA AAGGAGGTATATCGAATTCCAAAGAAAAGTCAAACCGAAAAGGAGAGCACAGTAACTGAACGAGGGAGGG ACACTGCTGCCTTCAGAGATCAAACAGCTCCAAAGACCCCTAACAGGTCGAGAGAGAGAGAGCCAGACAA GCAGTCTCAAAATAAAGAGAAAAGGAAACGCCGGGGCTCGCTGTCACCACCTTCTTCTGCGTATGAACGG GGAACAAAAAGGCCAGATGACAGGTATGATACACCAACTTCTAAAAAGAAAGTACGAATTAAAGACCGAA ACAAACTTTCTACAGAGGAGCGCAGGAAGTTGTTTGAACAAGAGGTAGCACAAAGGGAGGCTCAGAAGCA ACAGCAACAGATGCAGAACTTGGGGATGACATCACCACTACCCTTTGACTCTCTGGGTTATAATGCCTCT CATCACCCCTTTGCTGGGTACCCACCAGGTTATCCCATGCAAGCCTATGTGGATCCCAGCAACCCTAATG CTGGAAAGGTGCTTCTGCCTACACCTAGCATGGACCCTGTGTGCTCCCCTGCGCCTTATGATCACGCTCA GCCCTTGGTAGGACATTCTACAGAGTCTCTTGCTGCCCCTCCGTCTGTGCCAGTGGTACCACACGGGGCA GCCTCCGTGGAAGTTTCTAGTTCACAGTATGCAGCTCAGAGTGAAAGTGTGGTGCATCAAGACTCCAGTG TCCCTGGAATGCCAGTACAAACTCCAGGCCCAGTCCAAGGACAGAATTACAGTGTCTGGGATTCAAACCA ACAGTCTGTCAGTGTACAGCCCCAGTATTCTCCTGCCCAATCTCAAGCAACCATATATTACCAAGGACAG ACATGTTCAACTGTCTACGGTGTGACCTCTCCTTATTCACAGACAACTCCTCCAATTGTGCAGAGTTATG CCCAGCCAAGTCTTCAGTATATCCAGGGACAGCAGATTTTCACAGCTCACCCACAAGGAGTGGTGGTACA GCCAGCTGCAGCCGTGACTACAATAGTTGCACCAGGGCAGCCTCAGCCCTTACAGCCACCTGAAATGGTT GTAACAAATAATCTACTGGATCTGCCACCGCCCTCCCCTCCAAAACCAAAAACGATTGTTTTACCTCCCA ACTGGAAGACAGCCCGAGATCCTGAAGGGAAGATCTATTACTACCACGTAATCACGAGACAGACTCAGTG GGATCCTCCTACCTGGGAAAGCCCAGGAGATGATGCCAGCCTTGAGCATGAAGCTGAGATGGACCTGGGA ACCCCAACCTATGATGAAAACCCCATGAAGACATCAAAAAAGCCCAAGACAGCAGAAGCAGACACCTCCA GTGAGCTGGCAAAGAAAAGCAAAGAAGTATTCAGAAAAGAGATGTCTCAGTTCATTGTCCAGTGCCTGAA TCCTTACCGGAAACCTGACTGCAAGGTGGGGAGGATCACGACGACTGAAGATTTCAAGCACCTCGCGCGA AAGCTGACTCATGGAGTCATGAACAAGGAGCTGAAGTACTGTAAGAACCCTGAGGACCTGGAGTGCAACG AGAATGTGAAACACAAAACCAAGGAGTACATCAAGAAGTACATGCAGAAGTTTGGGGCTGTTTACAAACC TAAAGAGGACACTGAACTAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210372 representing NM_001108189 Red=Cloning site Green=Tags(s) MKSVPESLELDMKQDIVSNSLEEHTNQTLKEQADNALQKEDSHIGKEEEVSDGSKISLSSKKASSKKKSS QFEGTFLGSESDEDSVRTSSSQRSHDLKSSTSIEKERDFKKSSAPSKSEDLGKSSRSKTERDDKYFSYSK LERDTRYVSTRCRSERDRRRSRSRSRSDRVSRTSLSYSRSERSHYYDSERRYHRSSPYRERTRYSRPYTD NRARESSDSEDEYKKTYPRRTSAHSYRDLRTSSSYSKFDRDCKTETSYLEMERRGKYSSKLERESKRTSE HEAIKRCCSPPNELGFRRGSSYSKHDNNSTSRYKSALSKSISKSDKFKNSFCCTELNEENKQSHSFSLQT PCSKGSELRTINKIPEREKTGSPSPSNQLNDSPTFKKLDESPILKPEFIGHDSHESIKELELPKMKNDQL RNFCSIELNVNGSPETETDVATFRTSKPDAISMTSDDSVTGSEVSPLIKGCVLSSNGFQSVGRCRERDAD DACRQHNKSKSPFRETEPLLSPHHDKVMSLPVKTIDYSKTLIKEPVDKRHSCCKTKDSDRYCSPNESSEA ENREISSNCFVNVHLDSKTVVCDNRELTDQHSEFTCDGYKQSIGSTSSASLNHFDDLYESVGSSGISSLQ SLPSGIRCEENTSPALDTVQSKKGIDFLKYVGKETDVVSALPDSGKAFSSWENRHNMLSGQSLQESQEEG SSTLHERRGRSEVSLDEEEQRGHTHISDDSEVVFPYDLNLTMEDSDGVTYTLKCDSSGNAPEIVSTVHGD YSASSASSSDESDSEDTESDDSSIPRNRLQSVVVVPKNSTLPMEETSPCSSRSSQSYRHYSDHWEDGLES RRHAYEEKFESMASKGCSQTEKFFLHKGTERNPETSYSQFSRKIDNHLPEIAHSQSDGVDSTSHTDVKSD SVGHLNSEDAIRAKMSRPQELPVYCDDFEDLPNTSRQQMIFSNRPDSSRLGKAELSFSSSCDISRMDGLH SSEELRNLGWDFSQQERPTTTYQQPDSSYGTCGTHKYQQSAEHYGGTHDYWQGNGYWDPRSAGRPPGTGV VYDRIQGQVPDSLTDDREEEENWDQRSGSHFSSPSNKFFFHQKDKGSVQAPEISSNSIKDSLVINERKDF SKNFEKNDIKERGPPKKRRQELESDSESDGELQARKKVRVEIEQGQSSVPQHSELVGPSCAMDDFRDPQR WKEFAKLGKMPCYFDLIEENVYLTERKKNKSHRDIKRMQCECTPLSKDERAQGEVACGEDCLNRLLMIEC SSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLPSNTFVLEYCGEVLDHKEFKARVKEYARN KNIHYYFMALKNDEIIDATQKGNCSRFMNHSCEPNCETQKWTVNGQLRVGFFTTKLVPSGSELTFDYQFQ RYGKEAQKCFCGSANCRGYLGGENRVSIRAAGGKMKKERSRKKDSVDGELEALMENGEGLSDKNQVLSLS RLMVRIETLEQKLTCLKLIQNTHSQSCLKSFLERHGLSLLWIWMAELGDGRESNQKLQEEIIKTLEHLPI PTKNMLEESKVLPIIQRWSQTKTAVPQLSEGDGYSSENTSRAHTPLNTPDPSAKPSTEVDTDTPKKLIFR RLKIISENSMDSAVSDVTSELECKDGKEDLDQLETVTVEEDEELQSQQLLPSQLCESKVESESTIDVSKL PISEPEADTETEPKDSNGTKLEETIAEETPSQDEEEGVSDVESERSQEPPDKTVDISDLATKLLDSWKDL KEVYRIPKKSQTEKESTVTERGRDTAAFRDQTAPKTPNRSREREPDKQSQNKEKRKRRGSLSPPSSAYER GTKRPDDRYDTPTSKKKVRIKDRNKLSTEERRKLFEQEVAQREAQKQQQQMQNLGMTSPLPFDSLGYNAS HHPFAGYPPGYPMQAYVDPSNPNAGKVLLPTPSMDPVCSPAPYDHAQPLVGHSTESLAAPPSVPVVPHGA ASVEVSSSQYAAQSESVVHQDSSVPGMPVQTPGPVQGQNYSVWDSNQQSVSVQPQYSPAQSQATIYYQGQ TCSTVYGVTSPYSQTTPPIVQSYAQPSLQYIQGQQIFTAHPQGVVVQPAAAVTTIVAPGQPQPLQPPEMV VTNNLLDLPPPSPPKPKTIVLPPNWKTARDPEGKIYYYHVITRQTQWDPPTWESPGDDASLEHEAEMDLG TPTYDENPMKTSKKPKTAEADTSSELAKKSKEVFRKEMSQFIVQCLNPYRKPDCKVGRITTTEDFKHLAR KLTHGVMNKELKYCKNPEDLECNENVKHKTKEYIKKYMQKFGAVYKPKEDTELE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108189
ORF Size	6882 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108189.1, NP_001101659.1
RefSeq Size	8037 bp
RefSeq ORF	6885 bp
Locus ID	316013
MW	259.3 kDa

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