Erbb4 (NM_021687) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209714 representing NM_021687 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGGCGACGGGACTGTGGGTCTGGGGGAGCCTTCTGGTGGCAGCCAGGACCGTCCAGCCCAGCG CTTCTCAGTCAGTGTGTGCCGGAACAGAGAACAAACTGAGCTCTCTCTCTGATCTGGAGCAGCAGTACCG AGCCTTGCGCAAATACTATGAAAACTGCGAGGTAGTCATGGGCAACCTGGAGATCACCAGCATAGAGCAC AACCGGGACCTCTCCTTCCTGCGGTCTATCCGAGAAGTCACAGGCTATGTACTTGTGGCCCTCAACCAGT TTCGTTACCTGCCTCTGGAGAATTTACGCATTATTCGTGGGACAAAACTGTATGAAGATCGCTATGCCTT AGCAATATTCTTAAACTACAGGAAAGATGGCAACTTTGGACTTCAAGAACTGGGATTAAAGAACCTGACC GAAATACTAAATGGTGGAGTCTATGTAGACCAGAACAAATTCCTATGTTATGCTGATACTATACACTGGC AAGATATTGTTCGGAATCCATGGCCTTCCAACATGACTCTGGTGTCAACAATTGGAAGTTCTGGATGCGG AAGATGCCATAAGTCTTGCACTGGTCGATGCTGGGGACCCACAGAAAATCACTGCCAGACCTTGACAAGG ACTGTGTGTGCAGAACAATGTGATGGCAGGTGCTATGGACCCTACGTCAGTGACTGCTGCCATCGAGAAT GTGCCGGAGGCTGCTCAGGACCAAAAGACACTGACTGCTTTGCCTGCATGAACTTCAATGACAGTGGAGC ATGTGTTACTCAGTGTCCCCAAACGTTCGTCTACAATCCAACCACCTTTCAACTGGAACACAACTTCAAT GCAAAGTACACATATGGAGCATTCTGTGTTAAGAAATGTCCACATAACTTCGTGGTAGATTCCAGTTCTT GTGTACGAGCCTGCCCTAGTTCCAAGATGGAAGTCGAAGAAAATGGAATTAAAATGTGTAAGCCTTGCAC TGATATTTGCCCCAAAGCATGTGATGGAATCGGCACCGGATCCTTGATGTCTGCTCAGACTGTGGATTCC AGTAACATTGACAAATTCATAAACTGCACCAAGATCAACGGGAATCTCATCTTTCTTGTCACTGGCATTC ATGGGGACCCTTACAATGCTATTGACGCCATAGACCCAGAGAAACTGAATGTCTTTCGGACAGTCAGAGA AATAACAGGTTTCCTGAACATACAGACTTGGCCCCCAAATATGACAGATTTCAGTGTTTTCTCCAACCTT GTAACCATTGGAGGAAGAGTCCTCTACAGTGGTCTGTCATTGCTTATCCTCAAACAACAAGGTATCACTT CACTACAGTTCCAGTCTCTGAAGGAAATCAGTGCGGGCAATATCTACATCACGGACAACAGCAACCTGTG TTATTACCACACCATCAACTGGACAACACTGTTCAGCACCGTTAACCAGAGGATAGTGATCCGAGACAAC AGGAGGGCTGAGAACTGTACTGCTGAAGGGATGGTGTGTAACCACCTGTGTTCAAATGATGGTTGTTGGG GACCTGGGCCAGACCAGTGTCTGTCATGTCGGCGCTTCAGCAGGGGAAAGATCTGTATAGAGTCCTGCAA CCTTTATGATGGGGAGTTTCGAGAGTTTGAAAATGGCTCCATCTGTGTTGAGTGTGACTCCCAGTGTGAG AAAATGGAAGACGGACTCCTCACATGCCATGGACCGGGACCTGACAACTGTACAAAGTGTTCTCATTTTA AAGATGGTCCAAACTGTGTAGAGAAATGTCCAGATGTCCTACAGGGAGCAAACAGTTTCATATTTAAGTA CGCAGATCAGGATCGGGAGTGCCACCCTTGCCATCCAAACTGCACCCAGGGGTGTAACGGTCCCACTAGT CATGACTGCATTTACTACCCATGGACGGGCCATTCCACTTTACCACAACATGCTAGAACTCCACTGATTG CAGCCGGAGTCATTGGTGGGCTCTTCATCCTGGTCATCATGGCTCTGACATTTGCCGTTTATGTCAGAAG GAAGAGCATCAAAAAGAAACGCGCTTTGAGAAGATTCCTGGAGACCGAGTTAGTCGAGCCCTTAACCCCT AGTGGCACAGCACCCAATCAAGCTCAACTTCGAATTTTGAAGGAAACAGAGCTAAAGAGGGTAAAAGTCC TTGGCTCGGGAGCATTTGGAACCGTTTATAAAGGAATCTGGGTACCTGAAGGAGAAACTGTGAAAATCCC TGTGGCTATTAAGATCCTCAATGAGACAACTGGCCCCAAAGCCAATGTGGAGTTCATGGATGAGGCACTG ATTATGGCAAGTGTGGATCACCCACACCTAGTGCGTTTACTGGGTGTGTGTTTGAGCCCCACTATCCAGT TGGTTACTCAGTTGATGCCACATGGCTGCCTACTGGAATATGTCCACGAACACAAGGATAACATCGGATC ACAACTGCTGTTGAACTGGTGTGTCCAGATTGCTAAGGGAATGATGTATCTGGAGGAAAGGCGGCTTGTT CATCGGGATCTGGCAGCCCGCAATGTGTTGGTGAAATCTCCAAATCATGTTAAAATCACAGACTTTGGAC TGGCCCGGCTCTTGGAAGGAGATGAAAAAGAATACAATGCTGACGGTGGCAAGATGCCAATTAAATGGAT GGCTCTGGAATGTATACATTATAGGAAATTCACACATCAAAGCGATGTTTGGAGCTACGGTGTCACTATA TGGGAACTGATGACCTTTGGAGGAAAGCCCTATGATGGAATTCCAACGCGAGAAATCCCTGATTTATTAG AGAAGGGAGAGCGTTTGCCTCAACCTCCCATCTGCACTATTGACGTTTACATCGTCATGGTCAAATGTTG GATGATCGATGCTGACAGCAGACCTAAATTCAAAGAACTGGCTGCTGAGTTTTCAAGGATGGCTAGAGAC CCTCAAAGATACCTAGTAATTCAGGGGGATGATCGCATGAAGCTTCCCAGTCCAAACGACAGCAAATTCT TCCAGAATCTCTTGGATGAAGAGGATTTGGAAGATATGATGGACGCTGAGGAATATTTGGTCCCCCAGGC TTTCAATATCCCACCTCCTATCTACACATCCAGAACAAGAATTGACTCCAATAGGAGTGAAATTGGACAC AGCCCTCCTCCTGCCTACACCCCCATGTCGGGAAGTCAGTTTGTGTACCAGGATGGGGGTTTCGCTACAC AACAAGGAATGCCCATGCCCTACACAGCCACAACCAGCACCATACCAGAGGCTCCAGTCGCCCAGGGTGC AACGGCTGAGATGTTTGATGACTCCTGCTGTAATGGTACCCTGCGAAAGCCAGTGGTACCCCACGTCCAA GAGGACAGTAGCACTCAGAGGTATAGTGCCGACCCCACAGTGTTCGCCCCAGAACGGAACCCACGAGCAG AACTGGATGAAGAAGGCTACATGACTCCCATGCATGACAAGCCAAAACAAGAATATCTGAATCCTGTGGA AGAGAACCCTTTTGTGTCCCGGAGGAAGAATGGAGACCTTCAAGCTTTAGATAATCCAGAGTATCACAGC GCTTCCAGCGGTCCCCCCAAGGCAGAGGATGAGTACGTGAATGAGCCCCTTTATCTCAACACCTTCACCA ACGCCTTGGGAAATGCAGAGTACATGAAAAACAGCTTACTGTCTGTGCCAGAGAAAGCCAAGAAAGCATT TGACAACCCCGACTACTGGAACCACAGCCTGCCACCCCGGAGCACTCTTCAGCACCCAGACTACCTGCAG GAATACAGCACAAAATATTTTTATAAACAGAATGGACGGATCCGCCCTATTGTGGCAGAGAATCCTGAGT ACCTCTCAGAGTTCTCGCTGAAGCCAGGCACTATGCTGCCCCCTCCGCCCTACAGACACCGGAATACTGT GGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209714 representing NM_021687 Red=Cloning site Green=Tags(s) MKLATGLWVWGSLLVAARTVQPSASQSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEH NRDLSFLRSIREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRKDGNFGLQELGLKNLT EILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNMTLVSTIGSSGCGRCHKSCTGRCWGPTENHCQTLTR TVCAEQCDGRCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFN AKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDS SNIDKFINCTKINGNLIFLVTGIHGDPYNAIDAIDPEKLNVFRTVREITGFLNIQTWPPNMTDFSVFSNL VTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFSTVNQRIVIRDN RRAENCTAEGMVCNHLCSNDGCWGPGPDQCLSCRRFSRGKICIESCNLYDGEFREFENGSICVECDSQCE KMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDVLQGANSFIFKYADQDRECHPCHPNCTQGCNGPTS HDCIYYPWTGHSTLPQHARTPLIAAGVIGGLFILVIMALTFAVYVRRKSIKKKRALRRFLETELVEPLTP SGTAPNQAQLRILKETELKRVKVLGSGAFGTVYKGIWVPEGETVKIPVAIKILNETTGPKANVEFMDEAL IMASVDHPHLVRLLGVCLSPTIQLVTQLMPHGCLLEYVHEHKDNIGSQLLLNWCVQIAKGMMYLEERRLV HRDLAARNVLVKSPNHVKITDFGLARLLEGDEKEYNADGGKMPIKWMALECIHYRKFTHQSDVWSYGVTI WELMTFGGKPYDGIPTREIPDLLEKGERLPQPPICTIDVYIVMVKCWMIDADSRPKFKELAAEFSRMARD PQRYLVIQGDDRMKLPSPNDSKFFQNLLDEEDLEDMMDAEEYLVPQAFNIPPPIYTSRTRIDSNRSEIGH SPPPAYTPMSGSQFVYQDGGFATQQGMPMPYTATTSTIPEAPVAQGATAEMFDDSCCNGTLRKPVVPHVQ EDSSTQRYSADPTVFAPERNPRAELDEEGYMTPMHDKPKQEYLNPVEENPFVSRRKNGDLQALDNPEYHS ASSGPPKAEDEYVNEPLYLNTFTNALGNAEYMKNSLLSVPEKAKKAFDNPDYWNHSLPPRSTLQHPDYLQ EYSTKYFYKQNGRIRPIVAENPEYLSEFSLKPGTMLPPPPYRHRNTVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021687
ORF Size	3924 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021687.1, NP_067719.1
RefSeq Size	4060 bp
RefSeq ORF	3927 bp
Locus ID	59323
UniProt ID	Q62956
MW	147 kDa
Gene Summary	binds neuregulins; involved in epithelial-mesenchymal interactions during development; may play a role in tooth morphogenesis [RGD, Feb 2006]

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