Afdn (NM_013217) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Af6; Mllt4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209209 representing NM_013217 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGGCGGCCGCGACGAAGAGCGGCGGAAGCTGGCCGACATCATTCACCACTGGAACGCCAACC GGCTGGACCTGTTCGAGATCAGCCAGCCGACCGAGGATTTGGAGTTTCATGGAGTCATGAGATTTTATTT TCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTCTCTAGCACAGCAACCACTCAAGAT GTCATTGAAACACTGGCAGAGAAGTTCCGCCCTGATATGCGCATGCTCTCCTCTCCCAAGTACTCCCTCT ATGAAGTGCATGTCAGTGGAGAAAGAAGATTGGACATTGATGAGAAACCTCTAGTTGTACAGTTGAATTG GAACAAAGATGATCGGGAGGGTAGATTCGTCCTTAAGAACGAGAATGACGCCATTCCTGCCAAGAAGGCT CAGAGTAATGGACCTGAGAAGCAGGAGAAAGAAGGTGTTATTCAGAACTTCAAAAGAACTCTGTCAAAGA AAGAAAAGAAGGAAAAAAAGAAGAGAGAGAAGGAGGCTTTGAGACAAGCCTCTGATAAGGAAGAAAGGCC TTCTCAAGGGGATGACAGTGAGAATTCTCGTCTGGCTGCTGAAGTTTACAAAGACATGCCTGAAACCAGC TTTACTCGAACGATTTCTAATCCTGAAGTTGTTATGAAACGGCGGCGGCAGCAAAAACTGGAGAAGAGAA TGCAAGAGTTTCGGAGCTCAGATGGGCGGCCCGACTCGGGTGGAACACTGAGAATCTATGCAGACAGTTT AAAACCAAATATTCCATACAAGACAATCCTGCTGTCTACTACAGATCCTGCAGACTTTGCTGTAGCAGAG TCCTTAGAGAAGTATGGTCTGGAAAAAGAGAACCCCAAGGACTACTGCATTGCTCGGGTTATGCTTCCTC CTGGAGCCCAGCATTCTGATGAGAGAGGTGCTAAGGAAATAATTCTTGATGACGATGAATGCCCTTTACA AATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGACCACCGGACTAC ATTCCAAAGAAAATGAAGAAACATGTTGAAGGCAAGCCATTGAAGGGGAAAGATAGAGCTGACGGGTCTG GCTACGGCTCTGCTCTTCCTCCTGAGAAGCTGCCCTACTTAGTAGAGCTCAGCCCAGGGAGAAGGAATCA CTTTGCCTACTACAGCTATCACACTTACGAAGATGGCTCTGACTCCAGAGACAAGCCAAAGTTATACCGC CTTCAGTTAAGTGTGACTGAAGTTGGGACAGAGAAGTTCGATGACAATTCTATTCAGCTGTTTGGTCCTG GAATTCAGCCCCATCACTGTGATCTCACCAACATGGATGGAGTGGTCACTGTGACACCCAGAAGTATGGA TGCAGAGACCTATGTGGATGGTCAGCGCATCTCAGAGACCACAATGCTACAGAGTGGCATGAGACTGCAG TTTGGCACCTCGCATGTGTTTAAGTTTGTGGACCCCATCCAGGACCACGTTCTTTCCAAGAGATCTGTGG ATGGAGGCCTGATGGTCAAGGGCCCAAGACATAAACCTGGAGCCGTTCAAGAGACAACCTTTGAATTGGG AGGAGATATCCACAGTGGGACAGCATTGCCGGCAAGCAGGAGCACTACTAGATTGGACAGTGACAGGGTG TCCTCTGCCTCTAGCACAGCTGAGCGAGGGATGGTGAAGCCAATGATCCGACTGGACCAGGAGCAGGACT ACCGGCGAAGAGAAAGCAGGACTCAGGATGCTGCTGGGCCTGAACTGATGCTGCCTGCCAGCATTGAATT CCGGGAAAGCTCTGAAGACTCTTTTTTATCTGCCATTATAAACTATACCAATAGCTCTACAGTTCATTTT AAGTTGTCACCTACATATGTGTTATACATGGCATGCCGGTATGTATTGTCCAGCCAGCACAGGCCCGACA TCAGTCCTACAGAGCGGACACATAAGGCCATTGCTGTTGTGAACAAGATGGTGAGCATGATGGAAGGGGT CATCCAGGAAGTAGACCAGGTTGATCAGAAACAGAAGAACATTGCAGGAGCACTTGCTTTCTGGATGGCA AATGCATCTGAGCTTCTCAACTTCATCAAGCAGGACCGAGACCTTAGTCGGATTACTCTGGATGCCCAGG ATGTTCTAGCACACTTGGTTCAGATGGCATTTAAGTACTTGGTTCACTGCCTTCAGTCAGAACTTAATAA CTACATGCCAGCCTTTCTGGATGATCCCGAGGAGAACAGTCTGCAGAGACCAAAAATAGATGATGTGCTG CATACGCTCACAGGAGCCATGTCCTTGTTGCGGCGCTGCAGAGTCAATGCCGCCTTGACCATCCAGCTGT TCTCTCAGCTTTTCCACTTCATCAATATGTGGCTATTCAACAGACTGGTGACTGACCCAGATTCAGGCCT GTGTTCCCACTACTGGGGCGCAATTATCCGCCAGCAGCTGGGGCATATTGAAGCTTGGGCAGAGAAGCAG GGACTAGAGTTGGCAGCTGACTGTCACTTGAGCAGGATCGTGCAGGCTACTACGTTGCTTACAATGGATA AGTATGTACCTGATGACATTCCAAATATAAACAGCACATGCTTTAAGTTAAATTCTCTGCAGCTTCAAGC CCTCTTACAGAATTACCACTGTGCACCTGATGAGCCTTTTATTCCTACGGATCTCATAGAAAATGTCGTG GCTGTTGCTGAGAACACAGCTGATGAGCTTGCCCGCAGTGATGGCAGAGATGTGCAGTTAGAGGAGGACC CTGATCTACAACTGCCCTTTCTTCTGCCAGAAGATGGCTATTCCTGTGATGTTGTCAGAAACATTCCAAA TGGTTTACAAGAATTTTTAGACCCTCTGTGCCAGAGAGGATTTTGCAGGTTGGTTCCTCACACACGTTCT CCAGGTACCTGGACAATATATTTTGAAGGAGCAGATTATGAAAGTCACCTCATGCGTGAGAACACAGAAC TGACCCAGCCTCTGAGGAAGGAGCCTGAGGTAATCACTGTGACCCTGAAGAAGCAGAATGGAATGGGCCT CAGCATTGTTGCAGCAAAGGGTGCTGGCCAGGATAAACTGGGAATCTACGTTAAGTCCGTTGTCAAAGGA GGTGCTGCAGATGTGGATGGACGCCTAGCTGCTGGTGACCAACTCCTTAGTGTGGATGGACGAAGTCTGG TTGGACTTTCTCAGGAAAGGGCAGCAGAACTCATGACAAGAACCAGTTCCGTGGTAACACTGGAAGTTGC AAAGCAAGGCGCTATCTATCACGGACTGGCCACTCTCCTTAACCAGCCATCACCCATGATGCAGAGAATT TCAGATCGTCGTGGCTCAGGTAAACCCAGACCAAAGAGCGAAGGTTTTGAGCTCTATAATAATTCAGCTC AAAACGGCTCCCCAGAGAGTCCTCAGATGCCTTGGACAGAGTACAGTGAGCCAAAGAAGCTGCCTGGTGA CGACAGGCTGATGAAAAACAGAGCTGACCACCGTTCCAGCCCCAATGTGGCAAATCAGCCCCCTAGTCCT GGAGGGAAGAGTCCATATACCTCTGGAACAGCAGCTAAAATAACATCTGTCTCTACTGGAAACCTCTGCA CTGAGGAGCAGACCCCTCCACCTAGACCTGAAGCCTATCCTATCCCTACTCAGACATATACCAGAGAGTA TTTTACCTTTCCAGCTTCAAAGTCGCAGGATCGGATGGCTCCTGTTCAAAACCAGTGGCCAAATTATGAG GAAAAACCACACATGCACACAGAAAGTGATCATGCCAGTATTGCAATCCAGCGTGTTACCCGTTCCCAGG AAGAGCTTCGGGAAGAGAAGGTTTACCAACTTGAGCGTCATCGAGTAGAGTCAGGCATGGATCGCAAGTG TGACAGTGATATGTGGATCAATCAGAGCTCCTCAGTGGAATCTAGCACGTCTAGCCAGGAGCACCTGAAC CATTCCTCCAAGTCAGTTACCCCTGCTTCCACTCTGACCAAGAGTGGTCCTGGTCGTTGGAAAACCCCAG CAGCTGTGCTCCCCACCCCTGTGGCTGTCTCCCAACCCATTCGAACAGACCTCCCTCCACCACCACCTCC ACCACCTGCCCACTACACCAGTGATTTTGATGGTATTTCAATGGATTTGCCCCTCCCACCTCCTCCTGCC AACCAAGCAGCTCCCCAGTCTGCTCAGGTGGCTGCTGCAGAGAGGAAAAAGAGAGAGGAGCATCAGAGGT GGTATGAAAAAGAGAAGGCCCGCCTGGAGGAGGAACGAGAGAGAAAGCGCAGGGAGCAAGAGAGGAAGTT GGGGCAGATGCGCACTCAGTCCCTGAACCCTGCTTCCTTCTCTCCTTTGGCCACACAGGCCAAGCCAGAA AAGCCTTCCACACTGCAGAGGCCCCAAGAAACGGTCATTCGGGAGCTGCAGCCCCAGCAGCAGCCCCGTA CTATTGAGCGCAGGGATCTGCAGTACATAACCATCAGTAAAGAGGAGCTGTCCTCTGGGGATAGTCTGTC TCCAGACCCATGGAAACGAGATGCCAGGGAGAAGCTGGAGAAGCAACAGCAAATGCACATTGTAGACATG CTAAGCAAGGAGATCCATGAGCTGCAAAACAAGGGGGACCGTACTGCAGAGGAGAGTGACCGCCTGCGGA AGCTTATGCTGGAGTGGCAGTTCCAGAAGAGACTACAGGAGTCCAAGCAGAAGGATGAGGATGATGATGA AGAGGAAGATGATGATGTTGACACCATGCTGATCATGCAGCGACTGGAGGCGGAGCGGAGAGCCAGGTTG CAGGATGAAGAGCGCAGGCGCCAGCAACAGTTGGAAGAGATGCGGAAACGAGAAGTTGAAGACAGAGTAA GACAAGAGGAAGATGGACGCCATCAGGAGGAGGAGCGAGTAAAGAGAGATGCTGAAGAAAAGAGGCGACA GGAAGAAGGGTATTACAGCCGCCTAGAAGCTGAGAGGCGCAGACAGCATGAAGAGGCAGCACGCAGGTTG CTGGAGCCCGAAGAACCCGGGCTGAGCCGACCTCCACTTCCACAGGACTACGAGCCCCCATCCCAGTCCT CAGCACCCAGTGCCCCTCCTCCCCCACCTCAGCGAAATGCCTCCTACCTCAAAACACAGGTCCTCTCCCC AGACTCGCTGTTCACTGCCAAGTTTGTTGCGTATGATGATGATGATGAGGAGGAGAACTACGTCCCAGCA GGACCAAACTCTTATTCGGGATCTGCTGGCACAACTGCTGGAACTTATGATGCTCCTCGGGACACAAGAG AAAAACTCAGTAGAAGCCAAGATGCAGACTTACCTGGCAGTTCTGGAGCCCCTGAAAACCTGACATTTAG AGAGCGTCAGCGCCTCTTTTCACAAGGTCAAGATGTGTCAGACAAAGTGAAAGCTTCTCGTAAATTAACA GAGCTTGAGAATGAACTCAACACCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209209 representing NM_013217 Red=Cloning site Green=Tags(s) MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQD VIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKA QSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETS FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAE SLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDY IPKKMKKHVEGKPLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYR LQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQ FGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDIHSGTALPASRSTTRLDSDRV SSASSTAERGMVKPMIRLDQEQDYRRRESRTQDAAGPELMLPASIEFRESSEDSFLSAIINYTNSSTVHF KLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMA NASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVL HTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQ GLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVV AVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRS PGTWTIYFEGADYESHLMRENTELTQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKG GAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRI SDRRGSGKPRPKSEGFELYNNSAQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSP GGKSPYTSGTAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPVQNQWPNYE EKPHMHTESDHASIAIQRVTRSQEELREEKVYQLERHRVESGMDRKCDSDMWINQSSSVESSTSSQEHLN HSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPAHYTSDFDGISMDLPLPPPPA NQAAPQSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPASFSPLATQAKPE KPSTLQRPQETVIRELQPQQQPRTIERRDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDM LSKEIHELQNKGDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARL QDEERRRQQQLEEMRKREVEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRL LEPEEPGLSRPPLPQDYEPPSQSSAPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDDDDEEENYVPA GPNSYSGSAGTTAGTYDAPRDTREKLSRSQDADLPGSSGAPENLTFRERQRLFSQGQDVSDKVKASRKLT ELENELNTK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013217
ORF Size	5487 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013217.2, NP_037349.1
RefSeq Size	7656 bp
RefSeq ORF	5490 bp
Locus ID	26955
UniProt ID	O35889
MW	207.7 kDa
Gene Summary	may play a role in linking the actin cytoskeleton to the plasma membrane [RGD, Feb 2006]

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