Dscam (NM_133587) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209167 representing NM_133587 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATACTGGCTCTCTCCTTGTTCCAGAGCTTCGCGAATGTTTTCAGTGAAGAGCCCCACTCCAGCC TCTACTTTGTCAATGCATCGCTGCAAGAGGTAGTGTTTGCAAGCACGTCGGGGACCCTGGTGCCCTGCCC GGCTGCAGGCATCCCTCCTGTGACTCTCAGATGGTACCTAGCAACGGGCGAGGAGATCTACGATGTCCCT GGGATCCGCCACGTCCATCCCAATGGCACTCTCCAAATTTTCCCCTTTCCTCCTTCAAGCTTCAGCACCT TAATCCATGATAATACTTACTATTGCACAGCTGAAAACCCTTCAGGGAAAATTAGAAGTCAGGATGTCCA CATCAAGGCTGTTTTACGGGAGCCCTATACAGTCCGTGTGGAGGACCAGAAAACCATGAGAGGCAATGTC GCGGTGTTCAAGTGCATTATCCCCTCCTCGGTGGAGGCGTACGTCACTGTCGTCTCATGGGAGAAAGACA CGGTTTCACTTGTCTCAGGATCTAGATTTCTCATCACATCCACGGGAGCCTTGTATATTAAAGATGTACA GAACGAAGATGGGCTGTACAACTACCGCTGCATCACGCGGCACAGATACACAGGGGAGACGAGACAAAGC AACAGCGCGAGACTGTTCGTGTCAGACCCAGCGAACTCAGCCCCATCCATCCTGGACGGGTTTGACCACC GCAAAGCCATGGCAGGGCAGCGTGTGGAGCTGCCGTGCAAAGCTCTTGGGCACCCCGAGCCGGACTACCG CTGGCTGAAAGATAACATGCCCCTGGAACTTTCCGGAAGGTTCCAAAAGACAGTGACCGGGCTGCTCATT GAGAACAGCCGCCCTTCAGACTCAGGCAGCTACGTGTGTGAAGTATCCAACCGATACGGCACTGCCAAGG TGATAGGCCGCCTGTACGTGAAACAGCCACTGAAAGCCACCATCAGTCCCAGAAAGGTTAAAAGCAGTGT GGGCAGCCAGGTCTCCTTGTCCTGCAGTGTGACAGGAAACGAAGACCAGGAGCTCTCCTGGTACAGAAAT GGTGAAATCCTCAACCCTGGGAAAAACGTGAGGATCACAGGACTCAACCACGCGAACCTTATAATGGATC ACATGGTCAAGAGTGATGGGGGCGCCTACCAGTGCTTTGTTCGCAAGGACAAGCTATCTGCTCAAGACTA TGTCCAGGTGGTCCTTGAAGACGGAACTCCCAAAATCATTTCCGCCTTTAGCGAGAAAGTGGTGAGCCCG GCAGAGCCAGTGTCCCTCGTGTGTAATGTGAAGGGTACACCCTTGCCCACGGTCACCTGGACCCTGGACG ATGACCCCATCCTCAAGGGCAGCGGCCACCGCATCAGCCAAATGATCACGTCCGAGGGGAACGTGGTCAG CTACCTGAATATCTCCAGCTCCCAGGTCCGGGATGGGGGTGTCTACCGCTGCACTGCCAACAACTCGGCT GGAGTCGTCCTGTACCAGGCTCGAATAAACGTAAGAGGGCCGGCAAGCATCAGACCGATGAAAAACATCA CTGCGATAGCAGGGCGTGACACGTACATCCACTGCCGTGTGATTGGCTACCCTTATTACTCCATCAAGTG GTACAAGAACGCTAACCTGCTTCCTTTCAACCACCGCCAGGTGGCGTTTGAGAACAATGGGACTCTGAAA CTCTCAGATGTGCAGAAAGAAGTTGACGAGGGAGAGTACACATGTAACGTGCTGGTGCAGCCACAGCTCT CCACCAGCCAGAGTGTCCACGTGACAGTCAAAGTTCCTCCTTTTATCCAACCCTTTGAGTTCCCAAGATT CTCTATCGGTCAGCGGGTCTTCATCCCATGTGTGGTGGTCTCGGGGGACTTACCCATCACCATCACCTGG CAGAAGGATGGCCGGCCGATTCCAGCAAGCCTCGGTGTAACCATCGACAACATCGACTTCACCAGCTCCC TGAGGATCTCCAACCTCTCACTGATGCACAACGGGAATTACACCTGCATCGCGCGGAACGAGGCGGCCGC CGTGGAACACCAGAGCCAGCTGATTGTAAGAGTTCCCCCTAAGTTCGTGGTCCAGCCCCGGGACCAGGAT GGGATCTATGGCAAAGCAGTGATTCTCAATTGCTCTGCAGAGGGTTATCCTGTGCCTACAATTGTGTGGA AATTCTCAAAAGGTGCTGGGGTCCCCCAGTTCCAGCCAATTGCCCTGAATGGCAGAATCCAGGTCCTGAG TAATGGCTCACTCTTGATCAAGCATGTTGTGGAAGAAGACAGTGGCTACTACCTCTGCAAGGTCAGCAAC GATGTGGGCGCAGACGTCAGCAAGTCCATGTACCTCACGGTGAAAATTCCTGCCATGATAACCTCTTATC CCAACACCACCCTGGCCACTCAGGGTCAAAGGAAGGAGATGAGCTGCACAGCACACGGGGAGAAGCCCAT CATTGTCCGCTGGGAGAAAGAGGACAGGATCATTAACCCTGAAATGGCCCGTTACCTGGTGTCCACCAAG GAGGTGGGAGAGGAAGTGATTTCTACTCTGCAGATTTTGCCAACAGTGAGAGAAGATTCCGGCTTCTTCT CCTGCCATGCTATCAATTCATATGGGGAGGACCGCGGAATAATTCAGCTCACGGTGCAAGAACCCCCAGA CCCCCCCGAGATTGAGATCAAAGATGTCAAAGCCCGCACAATCACGCTCAGGTGGACCATGGGGTTTGAT GGCAACAGCCCCATCACAGGCTACGACATTGAGTGCAAAAATAAATCAGACTCCTGGGATTCTGCTCAAA GAACCAAAGATGTTTCCCCTCAGCTGAACTCGGCCACCATCATTGATATCCACCCTTCCTCCACCTACAG CATCCGCATGTACGCCAAGAACCGGATTGGCAAGAGTGAGCCCAGCAACGAGATCACCATCACGGCAGAT GAGGCAGCTCCTGATGGTCCACCTCAGGAAGTGCACCTGGAACCCACCTCATCTCAGAGTATCAGGGTTA CCTGGAAGGCTCCCAAGAAACATTTACAAAATGGGATCATACGTGGCTACCAGATAGGCTACCGGGAGTA CAGCACCGGGGGCAACTTCCAGTTCAACATCATCAGTATCGACACCACCGGGGACAGCGAAGTGTACACC CTGGACAACCTGAATAAGTTCACCCAGTATGGCCTAGTAGTACAGGCCTGCAACCGGGCCGGCACGGGAC CTTCTTCTCAGGAGATAATCACTACCACCCTGGAGGACGTACCCAGCTACCCTCCCGAAAATGTCCAAGC CATAGCAACCTCACCAGAAAGCATATCAATCTCCTGGTCCACGCTGTCCAAGGAGGCCTTGAATGGGATT CTCCAGGGGTTCAGGGTCATCTACTGGGCCAACCTCATAGACGGAGAGCTGGGCGAGATTAAGAACGTCA CCACCACGCAGCCTTCCTTGGAGTTGGATGGACTAGAGAAATACACCAACTACAGTATCCAGGTGCTGGC CTTCACCCGTGCAGGGGACGGCGTCCGGAGTGAGCAGATCTTTACCCGTACCAAGGAGGATGTTCCAGGT CCTCCTGCTGGTGTCAAGGCAGCAGCAGCCTCGGCCTCCATGGTCTTTGTGTCCTGGCTGCCCCCGCTCA AGCTGAATGGCATCATTCGGAAGTACACGGTGTTCTGCTCCCACCCCTACCCCACAGTCATCAGCGAGTT TGAAGCCTCCCCTGACTCATTTTCCTACAGAATTCCTAACCTGAGTCGGAATCGACAGTATAGCGTCTGG GTGGTGGCAGTGACTTCCGCCGGAAGAGGCAACAGCAGTGAGATCATCACCGTGGAGCCTCTGGCTAAAG CTCCTGCACGGATCCTCACGTTCAGTGGGACAGTGACTACTCCGTGGATGAAGGACATCGTCTTGCCGTG TAAAGCTGTTGGAGACCCATCACCTGCCGTAAAATGGATGAAGGATAGTAACGGGACGCCCAGCCTGGTG ACGATTGATGGCCGCAGAAGCATCTTCAGCAATGGGAGTTTCGTTATTCGCACGGTGAAGGCCGAGGACT CTGGCTACTACAGCTGTGTTGCCAATAACAACTGGGGATCAGATGAGATCATATTAAATTTGCAAGTACA AGTTCCACCAGATCAGCCTCGGCTCACCGTATCCAAGACGACATCCTCCTCCATCACCCTTTCCTGGCTT CCTGGAGACAATGGGGGCAGCTCCATCAGAGGCTACATCCTGCAATATTCCGAGGACAACAGTGAGCAGT GGGGGAGCTTTCCCATCAGCCCCAGCGAGCGTTCCTACCGCTTGGAAAACCTAAAGTGTGGGACATGGTA TAAGTTCACCCTTACTGCCCAAAATGGAGTGGGACCCGGACGCATAAGTGAAATCATAGAAGCCAAAACT CTGGGGAAAGAACCCCAGTTCTCCAAGGAGCAGGAGCTCTTCGCCAGCATCAATACCACCCGAGTGAGGC TGAATCTGATTGGCTGGAACGACGGCGGCTGTCCAATCACCTCATTCACTCTTGAGTACAGACCCTTCGG GACCACGGTCTGGACCACAGCTCAGCGGACCTCCCTTTCCAAGTCTTACATTCTGTATGACCTCCAAGAA GCCACATGGTATGAACTGCAGATGCGAGTGTGCAACAGCGCCGGCTGCGCAGAGAAGCAAGCCAACTTTG CCACGCTGAACTACGATGGCAGTACAATCCCTCCACTCATTAAGTCAGTTGTCCAAAGCGAAGAAGGGCT GACAACCAACGAAGGGCTCAAGATCCTCGTGACCATCTCCTGCATCCTGGTCGGGGTTCTGCTGCTCTTT GTGCTTCTGCTGGTGGTGCGAAGGAGACGGAGGGAGCAGAGGCTGAAGAGGCTGAGAGATGCAAAGAGTT TAGCTGAGATGCTCATGAGCAAGAACACACGGACTTCAGATACCTTGAGCAAACAGCAACAGACTCTGAG GATGCACATTGATATACCCAGGGCTCAGCTTTTGATTGAAGAGAGAGACACAATGGAGACCATAGATGAC CGCTCCACAGTCCTGTTAACGGATGCTGACTTTGGGGAGGCAGCCAAACAGAAGTCACTGACGGTGACTC ACACGGTGCATTACCAATCGGTGTCTCAGGCCACCGGGCCCCTAGTGGATGTCTCCGATGCTCGGCCAGG AACGAATCCCACCACCAGGAGGAATGCAAAGGCTGGACCCACAGCAAGAAACCGGTACGCCAGCCAGTGG ACCCTCAACAGACCCCACCCCACCATCTCAGCACACACTCTCACCACAGACTGGAGACTGCCTACCCCCA GGGCTACAGGCTCCGTGGACAAGGAAAGTGACAGCTACAGCGTCAGCCCATCACAAGACACAGACAGAGC AAGGAGCAGCATGGTCTCCACAGAAAGTGCCTCCTCTACCTACGAAGAACTGGCCAGGGCCTACGAGCAC GCCAAGATGGAGGAGCAGCTGAGGCACGCCAAGTTCACCATCACAGAGTGCTTCATATCCGACACGTCCT CCGAGCAGCTGACGGCAGGGACAAACGAGTACACGGACAGTCTGACCTCCAGTACCCCTTCAGAATCGGG GATCTGCAGATTCACTGCGTCTCCCCCCAAACCTCAGGATGGAGGTCGTGTGGTGAACATGGCGGTTCCA AAGGCCCATCGGCCAGGCGACCTCATACACCTGCCTCCATACCTGCGAATGGACTTCTTGTTAAACCGGG GCGCACCAGGCACCAGCAGGGACCTGAGCTTAGGACAAGCGTGCTTGGAACCCCAGAAAAGTCGGACCCT GAAGCGCCCCACGGTCCTTGAGCCCACCCCTATGGAGGCTTCCTCCTCCACCTCTTCCACGAGAGAAGGA CAGCAGTCGTGGCAACAAGGGGCCGTGGCCACCTTACCTCAGCGAGAGGGTGCAGAGCTGGGACAGGCAG CTAAAATGAGCAGCTCCCAAGAGTCGCTGCTGGACTCTCGGGGCCACTTGAAAGGAAACAATCCCTACGC AAAATCCTACACCTTGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209167 representing NM_133587 Red=Cloning site Green=Tags(s) MWILALSLFQSFANVFSEEPHSSLYFVNASLQEVVFASTSGTLVPCPAAGIPPVTLRWYLATGEEIYDVP GIRHVHPNGTLQIFPFPPSSFSTLIHDNTYYCTAENPSGKIRSQDVHIKAVLREPYTVRVEDQKTMRGNV AVFKCIIPSSVEAYVTVVSWEKDTVSLVSGSRFLITSTGALYIKDVQNEDGLYNYRCITRHRYTGETRQS NSARLFVSDPANSAPSILDGFDHRKAMAGQRVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVTGLLI ENSRPSDSGSYVCEVSNRYGTAKVIGRLYVKQPLKATISPRKVKSSVGSQVSLSCSVTGNEDQELSWYRN GEILNPGKNVRITGLNHANLIMDHMVKSDGGAYQCFVRKDKLSAQDYVQVVLEDGTPKIISAFSEKVVSP AEPVSLVCNVKGTPLPTVTWTLDDDPILKGSGHRISQMITSEGNVVSYLNISSSQVRDGGVYRCTANNSA GVVLYQARINVRGPASIRPMKNITAIAGRDTYIHCRVIGYPYYSIKWYKNANLLPFNHRQVAFENNGTLK LSDVQKEVDEGEYTCNVLVQPQLSTSQSVHVTVKVPPFIQPFEFPRFSIGQRVFIPCVVVSGDLPITITW QKDGRPIPASLGVTIDNIDFTSSLRISNLSLMHNGNYTCIARNEAAAVEHQSQLIVRVPPKFVVQPRDQD GIYGKAVILNCSAEGYPVPTIVWKFSKGAGVPQFQPIALNGRIQVLSNGSLLIKHVVEEDSGYYLCKVSN DVGADVSKSMYLTVKIPAMITSYPNTTLATQGQRKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVSTK EVGEEVISTLQILPTVREDSGFFSCHAINSYGEDRGIIQLTVQEPPDPPEIEIKDVKARTITLRWTMGFD GNSPITGYDIECKNKSDSWDSAQRTKDVSPQLNSATIIDIHPSSTYSIRMYAKNRIGKSEPSNEITITAD EAAPDGPPQEVHLEPTSSQSIRVTWKAPKKHLQNGIIRGYQIGYREYSTGGNFQFNIISIDTTGDSEVYT LDNLNKFTQYGLVVQACNRAGTGPSSQEIITTTLEDVPSYPPENVQAIATSPESISISWSTLSKEALNGI LQGFRVIYWANLIDGELGEIKNVTTTQPSLELDGLEKYTNYSIQVLAFTRAGDGVRSEQIFTRTKEDVPG PPAGVKAAAASASMVFVSWLPPLKLNGIIRKYTVFCSHPYPTVISEFEASPDSFSYRIPNLSRNRQYSVW VVAVTSAGRGNSSEIITVEPLAKAPARILTFSGTVTTPWMKDIVLPCKAVGDPSPAVKWMKDSNGTPSLV TIDGRRSIFSNGSFVIRTVKAEDSGYYSCVANNNWGSDEIILNLQVQVPPDQPRLTVSKTTSSSITLSWL PGDNGGSSIRGYILQYSEDNSEQWGSFPISPSERSYRLENLKCGTWYKFTLTAQNGVGPGRISEIIEAKT LGKEPQFSKEQELFASINTTRVRLNLIGWNDGGCPITSFTLEYRPFGTTVWTTAQRTSLSKSYILYDLQE ATWYELQMRVCNSAGCAEKQANFATLNYDGSTIPPLIKSVVQSEEGLTTNEGLKILVTISCILVGVLLLF VLLLVVRRRRREQRLKRLRDAKSLAEMLMSKNTRTSDTLSKQQQTLRMHIDIPRAQLLIEERDTMETIDD RSTVLLTDADFGEAAKQKSLTVTHTVHYQSVSQATGPLVDVSDARPGTNPTTRRNAKAGPTARNRYASQW TLNRPHPTISAHTLTTDWRLPTPRATGSVDKESDSYSVSPSQDTDRARSSMVSTESASSTYEELARAYEH AKMEEQLRHAKFTITECFISDTSSEQLTAGTNEYTDSLTSSTPSESGICRFTASPPKPQDGGRVVNMAVP KAHRPGDLIHLPPYLRMDFLLNRGAPGTSRDLSLGQACLEPQKSRTLKRPTVLEPTPMEASSSTSSTREG QQSWQQGAVATLPQREGAELGQAAKMSSSQESLLDSRGHLKGNNPYAKSYTLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133587
ORF Size	6039 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133587.1, NP_598271.1
RefSeq Size	6656 bp
RefSeq ORF	6042 bp
Locus ID	171119
UniProt ID	Q8VHZ8
MW	222.3 kDa
Gene Summary	mouse homolog is a neural cell adhesion molecule; plays a role in axon guidance [RGD, Feb 2006]

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