Bcl9l (NM_001106817) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208875 representing NM_001106817 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGATCCTGGCTAACAAGACAAGGTTACCCCACCCCAGGAGGAGAGAGGCTCCAGGGAGTCCACCGC TGTCCCCTCGAGGCCACTGCCCCCCTGCCCCAGCCAAGCCAATGCACCCAGAAAATAAATTGACCAATCA TGGCAAGACAGGGAATGGAGGGGCCCAATCCCAGCACCAGAATGTGAACCAAGGACCCACCTGCAACCTG GGCTCCAAGGGCGTGGGGGCGGGGAGCCATGGGGCCAAGGCCAACCAGATCTCACCCAGCAACTCAAGTC TGAAGAACCCCCAGGCAGGAGTGTCTCCTTTCAGCTCCCTCAAAGGCAAGGTGAAGCGAGAGCGGAGTGT GTCCGTGGACTCTGGAGAGCAGCGAGAAGCCGGAACTCCATCCCTGGATTCAGAGGCCAAAGAGGTGGCA CCCCGGAGTAAACGGAGGTGTGTGCTGGAGCGGAAGCAGCCATACAGTGGGGATGAATGGTGCTCTGGAC CAGACAGCGAGGAGGACGACAAGCCCATTGGGGCCACCCACAATTGTAATGTAGCAGACCCAGCCATGGT GGCCCCACAGTTGGGTCCTGGCCAAACTGCCCAACTGCCCCTCAGTGAGAACAGTGCACCAGGCCCCCAA CATGGCCCCCCGCCAGGCCTTCGGCCAGACGTCCCTGGGGGTGGGGGTGGGGGCGTCCCAGGAAAGCCTC CGTCTCAGTTCGTGTACGTCTTCACCACCCATCTGGCCAACACGGCGGCAGAAGCAGTGCTACAGGGCCG GGCAGAGTCCATCCTCGCCTACCACCAGCAGAACGTGCCCCGGGCCAAGCTGGATCAGGCCCCTAAAGTG CCACCCACCCCAGAACCACTACCCCTGAACACGCCATCAGCAGGTACACCACAGTCCCAGCCACCTCCGT TGCCACCGCCACCACCAGCCCCTGGCAGTGCCCCTCCTGCTCTGCCCCAGGAGGGGCCTCCTGAAGACAC CAGTCAGGACCTGGCCCCCAACTCAGTGGGAGCTGCCAGCACAGGTGGTGGGACTGGGGGCACCCACCCC AACACCCCAACGGCTGCCACTGCTAACAACCCTCTGCCTCCTGGAGGAGACCCTGGCAGTGCTCCTGGCT CTGCCCTATTGGGCGAGGCCACGCCCACAGGAAATGGGCAGAGGAACCTGGTGGGCTCTGAGGGCCTGTC CAAAGAGCAGCTGGAGCACCGTGAGCGCTCCCTCCAGACACTTCGAGACATAGAGAGGCTGCTGCTCCGC AGCGGGGAGACTGAGCCCTTCCTCAAGGGGCCCCCTGGAGGAGCTGTTGAGGGAGGCCCGCCCGCACAAG CGCCCTCTGCCGCTCAGCAGCCTCCCTCCGCCCCTCCTCCGGGGCTGAAGAAGTACGAGGAACCTCTGCA GTCAATGATTTCACAGACACAGAGCCTTGGAGGCCCCCCATTGGAGCACGAGGTGCCGGGGCACCCTCAG GGCGGAGACATGGGACAGCAAATGAACATGATGATGCAGAGGCTGGGCCAGGACAGTCTGACACCTGAGC AGGTGGCCTGGCGCAAACTGCAGGAAGAGTACTACGAGGAGAAGCGGCGGAAAGAGGAGCAGATTGGATT GCATGGGGGCCGCCCTCTGCAGGACATGGTGGGAATGGGGGGCAACCAGATGCAAAGGGTGCCTGGCTTT GGAGGTATGCAGAGTATACCCATGGAAGTACCCATGAATGCCATGCAGAGACCTGTAAGGCCGGGCATGG CCTGGAATGAAGACTTGCCCCCTATTGGGGGACCCAGCAACTTTGCCCAGAATGCTGTGCCCTATCCAGG TGGGCAGGGTGAGGCAGAGCGGTTCATGACCCCTCGTGTCCGGGAGGAGCTGCTGAGGCACCAGTTGCTG GAGAAGCGGTCAATGGGCATGCAGCGGCCCCTGGGCATGGCGGGTAGTGGCATGGGACAGAGCATGGAAA TGGAACGGATGATACAGACACATCGACAGATGGACCCTACCATGTTCCCAGGGCAGATGACTGGAGGAGA TGGTCTGGCTGGCACTCCCATGGGCATAGAGTTCGGTGGAGGCCGGGGCCTCCTGAGCCCTCCAATGGGA CAGTCTGGGCTGAGGGAGGTAGACCCACCCATGGGACCGGGCAACCTCAACATGAACATGAATGTCAACA TGAACATGAACATGAACCTGAATGTGCAGATGACGCCACAGCAGCAGATGCTGATGTCACAGAAGATGCG AGGCCCTGGAGACATGATGGGTCCTCAGGGCCTCAGTCCTGAGGAGATGGCTCGAGTTCGGGCCCAGAAC AGTAGTGGCATGATGGCGGGTCCACAGAAGATGCTCATGCCTTCTCAGTTTCCCAACCAGGGCCAGCAGG GATTCTCTGGGGGCCAGGGACCCTACCAAGCCATGCCCCAGGACATGGGCAACACTCCAGACATGTTTAG CCCTGATCAGAGTTCAGTGCCCATGGGCGCTGTGGGCACCGCCCGGCTCAGCCATATGCCTCTGCCCCCT GCATCCAATCCCCCTGGGTCTGTGCACTCAGCCCCCAATAGGGGGCTTGGCAGGCGGCCTTCAGATCTCA CCATCAGTATTAATCAGATGGGCTCACCAGGCATGGGGCATCTGAAGTCACCCACCCTTAGCCAGGTGCA CTCCCCGCTGGTCACCTCACCCTCTGCCAACCTTAAGTCACCCCAGACTCCCTCACAGATGGTACCCTTG CCTTCTGCCAACCCACCAGGACCTCTCAAGTCACCCCAGGTCCTCAGCTCTTCCCTCAGTGTGCGTTCAC CCACTGGCTCACCCAGCAGGCTCAAGTCTCCCTCCATGGCGGTGCCTTCTCCGGGCTGGGTCGCCTCTCC CAAGACAGCCATGCCTAGTCCTGGGGTCTCCCAGAATAAACAGCCACCTCTCAACATAAACTCTTCCTCT ACCCTGGGCAACCTGGAACAGGGTGCTCTCCCACCTGCTGGCCCCCGGAACAGCTCCTCAGCTCCTCCCG CCAACCCTCCCAGCAGCCTCATGAATCCCAGCCTACCGTTCACATCCTCCCCAGACCCCACGCCTTCCCA GAACCCTCTGTCACTGATGATGTCTCAGATGTCCAAGTATGCCATGCCCAGCTCTACCCCGCTGTACCAC AATGCCATCAAGACCATCGCCACCTCAGATGACGAGTTGCTGCCTGACCGGCCCCTGCTACCCCCACCCC CACCACCGCAGGGCTCTGGGCCAGGTATCAGCAACAACCAGCCCAACCAGATGCATATGAACCCTGCTGC TGCCCAGAGCCCCATGGGCATGAACTTGCCAGGCCAGCAGCCCCTGTCCCATGAGCCTCCACCTACTATG TTGCCCTCCCCCACCCCTCTGGGGTCCAACATTCCACTGCACCCCAATGCACAGGGGACTGGGGGCCCTT CTCAAAACTCAATGATGATGGCCCCAGGAGGCCCAGACTCCCTAAATACCCCTTGTGGCCCTGTGCCCAG CTCTTCCCAGATGATGTCCTTTCCTCCTCGGCTGCAGCAACCCCATGGTGCCATGGCCGCCACTGGGGGT GGGGGGCCTGGCCTGCAGCAGCACTACCCTTCAGGCATGGCCCTGCCCCCTGAGGACCTGCCCAACCAGC CACCTGGTCCCATACCCTCCCAGCAGCACCTGATGGGCAAAGGCATGGCTGGCCGCATGGGCGATGCGTA CCCACCGGGGGTACTCCCTGGGGTGGCATCGGTACTGAATGACCCAGAGCTGAGTGAGGTGATCCGGCCC ACCCCTACCGGCATTCCTGAGTTCGACTTATCCAGAATCATCCCCTCTGAGAAACCGAGCAGCACCCTCC AATACTTCCCCAAGAACGAGAACCAGCCCCCCAAGGCCCAGCCCCCCAATCTGCACCTCATGAACCTGCA GAACATGATGGCGGAGCAGGCCCCGTCTCGGCCCCCCAACCTCCCAGGCCAACAGGGGGTCCAGCGGGGT CTCAGCATGTCCATGTGCCACCCTGGACAGATGTCCTTGCTGGGCAGGACAGGTGTGCCCCCACAGCAGG GCATGGTGCCCCACGGCCTGCACCAGGGGGTCATGTCCCCTCCACAGGGCCTCATGACCCAGCAGAATTT TATGCTGATGAAACAGAGGGGCGTGGGGGGTGAGGTCTACACCCAGCCTCCCCACATGCTCTCCCCACAG GGCTCCCTCATGGCCCCCCCACCCCAGCAGAACCTCATGGTGTCCCACCCTCTGCGGCAGCGCAGTGTGT CTCTGGACAGCCAGATGGGCTACCTCCCAACGCCGGGCGGCATGGCCAATCTACCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208875 representing NM_001106817 Red=Cloning site Green=Tags(s) MRILANKTRLPHPRRREAPGSPPLSPRGHCPPAPAKPMHPENKLTNHGKTGNGGAQSQHQNVNQGPTCNL GSKGVGAGSHGAKANQISPSNSSLKNPQAGVSPFSSLKGKVKRERSVSVDSGEQREAGTPSLDSEAKEVA PRSKRRCVLERKQPYSGDEWCSGPDSEEDDKPIGATHNCNVADPAMVAPQLGPGQTAQLPLSENSAPGPQ HGPPPGLRPDVPGGGGGGVPGKPPSQFVYVFTTHLANTAAEAVLQGRAESILAYHQQNVPRAKLDQAPKV PPTPEPLPLNTPSAGTPQSQPPPLPPPPPAPGSAPPALPQEGPPEDTSQDLAPNSVGAASTGGGTGGTHP NTPTAATANNPLPPGGDPGSAPGSALLGEATPTGNGQRNLVGSEGLSKEQLEHRERSLQTLRDIERLLLR SGETEPFLKGPPGGAVEGGPPAQAPSAAQQPPSAPPPGLKKYEEPLQSMISQTQSLGGPPLEHEVPGHPQ GGDMGQQMNMMMQRLGQDSLTPEQVAWRKLQEEYYEEKRRKEEQIGLHGGRPLQDMVGMGGNQMQRVPGF GGMQSIPMEVPMNAMQRPVRPGMAWNEDLPPIGGPSNFAQNAVPYPGGQGEAERFMTPRVREELLRHQLL EKRSMGMQRPLGMAGSGMGQSMEMERMIQTHRQMDPTMFPGQMTGGDGLAGTPMGIEFGGGRGLLSPPMG QSGLREVDPPMGPGNLNMNMNVNMNMNMNLNVQMTPQQQMLMSQKMRGPGDMMGPQGLSPEEMARVRAQN SSGMMAGPQKMLMPSQFPNQGQQGFSGGQGPYQAMPQDMGNTPDMFSPDQSSVPMGAVGTARLSHMPLPP ASNPPGSVHSAPNRGLGRRPSDLTISINQMGSPGMGHLKSPTLSQVHSPLVTSPSANLKSPQTPSQMVPL PSANPPGPLKSPQVLSSSLSVRSPTGSPSRLKSPSMAVPSPGWVASPKTAMPSPGVSQNKQPPLNINSSS TLGNLEQGALPPAGPRNSSSAPPANPPSSLMNPSLPFTSSPDPTPSQNPLSLMMSQMSKYAMPSSTPLYH NAIKTIATSDDELLPDRPLLPPPPPPQGSGPGISNNQPNQMHMNPAAAQSPMGMNLPGQQPLSHEPPPTM LPSPTPLGSNIPLHPNAQGTGGPSQNSMMMAPGGPDSLNTPCGPVPSSSQMMSFPPRLQQPHGAMAATGG GGPGLQQHYPSGMALPPEDLPNQPPGPIPSQQHLMGKGMAGRMGDAYPPGVLPGVASVLNDPELSEVIRP TPTGIPEFDLSRIIPSEKPSSTLQYFPKNENQPPKAQPPNLHLMNLQNMMAEQAPSRPPNLPGQQGVQRG LSMSMCHPGQMSLLGRTGVPPQQGMVPHGLHQGVMSPPQGLMTQQNFMLMKQRGVGGEVYTQPPHMLSPQ GSLMAPPPQQNLMVSHPLRQRSVSLDSQMGYLPTPGGMANLPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106817
ORF Size	4329 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106817.1, NP_001100287.1
RefSeq Size	5711 bp
RefSeq ORF	4332 bp
Locus ID	300673
MW	151.3 kDa

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