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Cyp7a1 (NM_012942) Rat Tagged ORF Clone
CAT＃: RR208359
- TrueORF®
Cyp7a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1), (10 ug)
"NM_012942" in other vectors (3)
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CNY 6,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CHAP; CYP7; CYP7S1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208359 representing NM_012942
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACTATTTCTTTGATTTGGGGAATTGCCGTGTTGGTGAGCTGTTGCATATGGTTTATTGTTGGAA TAAGGAGAAGGAAAGCTGGTGAACCTCCTTTGGAGAACGGGTTGATTCCGTACCTGGGCTGTGCTCTGAA ATTTGGATCTAATCCTCTTGAGTTCCTAAGAGCTAATCAAAGGAAGCATGGTCACGTTTTTACCTGCAAA CTGATGGGGAAATATGTCCATTTCATCACAAACTCCCTGTCATACCACAAAGTCTTATGTCATGGAAAAT ATTTTGACTGGAAAAAATTTCATTACACTACTTCTGCGAAGGCATTTGGACACAGAAGCATTGACCCAAA TGATGGAAATACCACGGAAAATATAAACAACACTTTTACCAAAACCCTCCAGGGAGATGCTCTGTGTTCA CTTTCTGAAGCCATGATGCAAAACCTCCAATCTGTCATGAGACCTCCTGGCCTTCCTAAATCAAAGAGCA ATGCCTGGGTCACGGAAGGGATGTATGCCTTCTGTTACCGAGTGATGTTTGAAGCTGGCTATCTAACACT GTTTGGCAGAGATATTTCAAAGACAGACACACAAAAAGCACTTATTCTAAACAACCTTGACAACTTCAAA CAATTTGACCAAGTCTTTCCGGCACTGGTGGCAGGCCTTCCTATTCACTTGTTCAAGACCGCACATAAAG CTCGGGAAAAGCTGGCTGAGGGATTGAAGCACAAGAACCTGTGTGTGAGGGACCAGGTCTCTGAACTGAT CCGTCTACGTATGTTTCTCAATGACACGCTCTCCACCTTTGACGACATGGAGAAGGCCAAGACGCACCTC GCTATTCTCTGGGCATCTCAAGCAAACACCATTCCTGCAACCTTTTGGAGCTTATTTCAAATGATCAGGA GTCCTGAAGCAATGAAAGCAGCCTCTGAAGAAGTGAGTGGAGCTTTACAGAGTGCTGGCCAAGAGCTCAG CTCTGGAGGGAGTGCCATTTACTTGGATCAAGTGCAACTGAATGACCTGCCGGTACTAGACAGCATCATC AAGGAGGCTCTGAGGCTTTCCAGTGCATCCTTGAATATCCGCACAGCTAAGGAGGACTTCACTCTCCATC TTGAGGACGGTTCCTATAACATCCGAAAAGATGACATGATAGCTCTTTATCCACAGTTAATGCACTTGGA TCCTGAAATCTACCCAGACCCTTTGACTTTCAAATATGACCGGTACCTTGATGAAAGCGGGAAAGCAAAG ACCACCTTCTACAGTAATGGAAACAAGCTGAAGTGTTTCTACATGCCCTTCGGATCAGGCGCGACAATAT GTCCTGGAAGACTCTTTGCCGTCCAAGAAATCAAGCAGTTTTTGATCCTGATGCTCTCCTGCTTTGAACT GGAGTTTGTGGAGAGCCAAGTCAAGTGTCCCCCTCTAGACCAGTCCCGGGCAGGCTTGGGAATTTTGCCA CCACTACATGATATTGAGTTTAAATATAAACTGAAACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208359 representing NM_012942
Red=Cloning site Green=Tags(s) MMTISLIWGIAVLVSCCIWFIVGIRRRKAGEPPLENGLIPYLGCALKFGSNPLEFLRANQRKHGHVFTCK LMGKYVHFITNSLSYHKVLCHGKYFDWKKFHYTTSAKAFGHRSIDPNDGNTTENINNTFTKTLQGDALCS LSEAMMQNLQSVMRPPGLPKSKSNAWVTEGMYAFCYRVMFEAGYLTLFGRDISKTDTQKALILNNLDNFK QFDQVFPALVAGLPIHLFKTAHKAREKLAEGLKHKNLCVRDQVSELIRLRMFLNDTLSTFDDMEKAKTHL AILWASQANTIPATFWSLFQMIRSPEAMKAASEEVSGALQSAGQELSSGGSAIYLDQVQLNDLPVLDSII KEALRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDMIALYPQLMHLDPEIYPDPLTFKYDRYLDESGKAK TTFYSNGNKLKCFYMPFGSGATICPGRLFAVQEIKQFLILMLSCFELEFVESQVKCPPLDQSRAGLGILP PLHDIEFKYKLKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012942 |
| ORF Size | 1509 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012942.2, NP_037074.1 |
| RefSeq Size | 3544 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 25428 |
| UniProt ID | P18125 |
| MW | 56.9 kDa |
| Gene Summary | catalyzes the conversion of cholesterol, NADPH, and oxygen to 7-alpha-hydroxycholesterol, NADP(+) and water; plays a role in bile acid biosynthesis and cholesterol metabolism [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208359 | Cyp7a1 (untagged ORF) - Rat cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1), (10 ug) |
CNY 6,460.00 |
|
| RR208359L3 | Lenti ORF clone of Cyp7a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1), (10 ug) |
CNY 8,360.00 |
|
| RR208359L4 | Lenti ORF clone of Cyp7a1 (mGFP-tagged ORF) - Rat cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1), (10 ug) |
CNY 9,120.00 |
